Summary of the current status of the work of TUM-BO Andreas Gattinger, Matthias Weiss, Michael Schloter GSF-Research Center for Environment and Health.

Slides:

Advertisements

Similar presentations

Working Group on Soil Biodiversity Dr Jonathan Leake.

Advertisements

Lipids 2 Phospholipids and Steroids. Phospholipids  The structure of phospholipids is based on the structure of triglycerides but the third hydroxyl.

E COLOGIE ET E COPHYSIOLOGIE F ORESTIERES UMR 1137 INRA UHP.

Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A.

RECIPE - Munchen May 2005 ECOBIO. Influence of vegetation cover and age of regeneration stages on C and N soluble and microbial variables K-W test P-ValuesSOCSONSOC/SON.

Bottom-up methane dynamics along the degradation of permafrost: A case study on microbial communities of the methane cycle in a collapsing sub-Arctic palsa.

Biochemistry Part V Lipids. Lipids A class of organic molecules that are insoluble in water A class of organic molecules that are insoluble in water There.

Does Biological Diversity Control Ecosystem Function?

LIPIDS AND MEMBRANES. Fatty acids Hydrocarbon chain (saturated or un-) Carboxylic acid group C C HH HH.

Chapter 17 Prokaryotic Taxonomy How many species of bacteria are there? How many species can be grown in culture? Bergey’s Manual Classification Schemes.

Chapter 5 Membrane composition and fluidity.. You Must Know Why membranes are selectively permeable. The structure and function of membrane proteins.

LIPID REVIEW Dr. E. Lipids A. Hydrophobic B. Hydrophillic C. Nonpolar D. Polar E. Both A & C F. Both A and C.

Lipid Review What are the four examples of lipids? 2.

Insoluble Biological Molecules

Do NOW: What does this picture mean to you? There are two categories of compounds: Organic Inorganic.

Lipids By Umair, Manjit, and Rajat.  Lipids are a biological macromolecule composed of hydrogen, carbon and oxygen atoms  However the ratio of oxygen.

Lipid metabolism By Dr. Hoda Gad. OBJECTIVES BY THE END OF THESE LECTURES, STUDENT SHOULD BE ABLE TO:  Understand the structure of lipids including 

LIPIDS. Where can you find lipids? Lipid Functions and Properties Energy storage – Better source of energy than carbs Cushioning Insulation Lipids are.

Summary of the current status of the work of TUM-BO Scientists: Andreas Gattinger, Michael Schloter, Alexandra Hagn (DNA), Ursula Bausenwein (Socioeconomics)

Lipid. The function of lipid Lipids are a class of biological molecules defined by low solubility in water and high solubility in nonpolar solvents. Function:

COMMUNITY ECOLOGY cont pp Warm-up 4/2: Define Inter and Intraspecies competition. Which has greater competition? Explain Explain why parasites.

Phospholipid Fatty Acid Analysis Phospholipids are essential membrane components of all living cells Viable microbes have an intact membrane which contains.

Context, tests and considerations for field measurements (WP I) … RECIPE.

6.3 Structure of Lipids Chapter 6 ~ The Chemistry of Life Section 6.3 p

Deciphering the structure and function of complex microbial communities is a central theme in microbial ecology.

Lipids Triglycerides Phospholipids Sterols Lipids Lipids are a class of nutrients that includes: –Triglycerides (fats and oils) –Phospholipids –Sterols.

Summary of the current status of the work of TUM-BO Scientists: Andreas Gattinger, Michael Schloter Technicians: Franz Buegger (IRMS, plant labelling),

LIPIDS The Molecules of Cells CH 3. Lipids Lipids include fats, which are mostly energy-storage molecules Lipids include fats, which are mostly energy-storage.

AS Biology Core Principles Lipids. Aims o Elements of lipids o Structure of glycerol & fatty acids o Condensation reactions to form triglycerides o Phospholipids.

Cell membrane: Fluid mosaic. What Are Characteristics Of The Cell Membrane? Selectively permeable Composed of –Lipids –Proteins.

Organic Compounds!! LIPIDS. What makes McDonald ’ s fries taste soooo good? ?

Diploma thesis (Spanien – Österreich): Title of the Project: “Effect of burning of Mediterranean macchia on ecosystem nitrogen stocks and the soil-atmosphere.

Diversity of Soil Microbes. Approaches for Assessing Diversity Microbial community Organism isolation Culture Nucleic acid extraction Molecular characterization.

PLFA/FAME Analyses for Microbial Community Assessment

Craigiebuckler, Aberdeen, AB15 8QH, UK RECIPE Charquemont Progress meeting /10/2003 UK progress Rebekka Artz Stephen Chapman Colin Campbell.

Earth, Ecological, & Environmental Sciences

Kingdom Archaebacteria

6.4 The Building Blocks of Life

Cells Unit Objective B-Cell membrane and transport Cell membranes are selectively permeable due to their structure. –Cell membranes separate the internal.

Fatty Acid Recovery and Identification in Mars Analogue Soil Samples Kimberly Lykens Mentor: Michael Tuite Jet Propulsion Laboratory Planetary Chemistry.

3.1 Significance 2 major functions of lipids – Energy storage by nonpolar lipids – Membrane function by polar lipids Also form micelles Signal molecules.

New INSTAAR and Geological Sciences Facilities: 1. Organic and Isotope Geochemistry Laboratory 2. CU Boulder Earth Systems Stable Isotope Laboratory Julio.

Maria Bañuelos University of Redlands University of Oregon

Lipids Pg Objective: I can identify and classify different types of lipids based on their molecular structure and relate it to my health.

Cell membrane: Fluid mosaic

CHAPTER 6 Cont’d – THE BUILDING BLOCKS OF LIFE

LIPIDS Varied in structure

Activity #8: Lipids.

Chapter 3: Biochemistry

COMMUNITY ECOLOGY cont

Macromolecule -- Lipids

KEY CONCEPT Carbon-based molecules are the foundation of life.

Biogeochemistry of Wetlands

Membrane composition and fluidity.

Carbon Based Molecules

AS Biology Core Principles

Organic Compounds!! LIPIDS.

الدهــــــــون Lipids.

Correlation plot of the 26 assayed FAs ordered into major subfraction categories. Correlation plot of the 26 assayed FAs ordered into major subfraction.

INDICATORS OF REGENERATION IN AN ABANDONED CUT-OVER SCOTTISH PEATLAND

Lipids Made up of C, H and O Can exist as fats, oils and waxes

Carbs ENERGY Structural support of plants.

Lipids Major Organic Compounds.

The domain Archaea in human mucosal surfaces

Relevant Biomarkers of Inflammation and Their Possible Role in Atherosclerosis Arpita Basu, et al. ATVB, 2006; 26;

A manifesto for microbial genomics

AS Biology Core Principles

Organic/Bio Chemistry

Presentation transcript:

Summary of the current status of the work of TUM-BO Andreas Gattinger, Matthias Weiss, Michael Schloter GSF-Research Center for Environment and Health Technical University of Munich Institute of Soil Ecology D Neuherberg

1. Adaptation of protocols for lipid biomarker analyses to peat (bog) samples 2. Adaptation of protocols for DNA analyses to peat (bog) samples 3. Growing of keystone plant species in the greenhouse for plant litter labelling 4. Installation of the new analytical device GC/MS-C-IRMS for compound specific isotope analysis Summary of the current status of the work of TUM-BO

1. Adaptation of protocols for lipid biomarker analyses to peat (bog) samples

Determination of microbial communites Problem: less than 5% of the estimated total soil microflora can be cultivated Solution: cultivation-independent approaches enable the characterization of microbial communiteis in situ: PLEL derived hydrocarbons genotypic approach: (example archaeal 16S rDNA, separation with SSCP ) phenotypic approach: (side chain analysis of polar membrane lipids)

methanogens The biosphere of soil (mod. Version, M. Schmid, GSF) Archaeal kingdoms (Reiche): - Euryarchaeota - Crenarchaeota - (Korarchaeota) - (Nanoarchaeota)

Archaea etherlinked isoprenoids (PLEL): - saturated short chain: i20:0 - saturated long chain: i40:0 - cyclic long chain: i40:0-cy - unsaturated short chain: i20:1 Side chain analysis of polar membrane lipids of Bacteria Eucarya and Archaea in soil Bacteria & Eucarya esterlinked fatty acids (PLFA): - saturated (SATFA) - monounsaturated (MUFA) - polyunsaturated (PUFA) - hydroxysubstituted (PLOH) Bacteria & Eucarya non-esterlinked fatty acids (NEL-PLFA): - hydroxysubstituted (UNOH) - unsubstituted fatty acids (UNSFA)

Extended phospholipid analysis x Bodenökologie

Microbial biomarker composition in a Scottish “test” peat core

Lipid biomarker ratios in a Scottish “test” peat core

MOB and archaeal biomarker in a Scottish “test” peat core

2. Adaptation of protocols for DNA analyses to peat (bog) samples together with Rebekka Artz, Steve Chapman/MLURI

3. Growing of keystone plant species in the greenhouse for plant litter labelling

Plants were taken by A. Siegenthaler et al. at the French/Suisse site: 4 plant species: 2 mosses (Sphagnum, Polytrichum), 2 grasses (Eriophorum vaginatum, Eriophorum angustifolium) were grown at the GSF greenhouse for estimating growth conditions and biomass production climatic conditions:

4. Installation of the new analytical device GC/MS-C- IRMS for compound specific isotope analysis

Simultaneous identification and quantification of PLFA/PLEL from environmental samples and their corresponding 12 C/ 13 C ratios by GC/MS-C-IRMS MS (DSQ) IRMS (DeltaPlus Advantage ) 20% of the analyte 80% of the analyte

delta 13 C values in PLFA/PLEL from test core samples varied between -26 and -32 % 0 (natural abundance): due to dilution effects with the peat soil and 1-2% measurement inaccuracy, an enrichment with 13 C in the plant litter of at least 70 % 0 PDB is needed: Which quality of CO 2 gas for plant labelling?