MBV2010/BIO2140 Colloquium, February 5. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please.

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MBV2010/BIO2140 Colloquium, February 5

RULES Multiple choice Only one correct answer seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

Example 4789 x 9548 = ?? A B C D

Example 4789 x 9548 = ?? 0

Nucleic acids were first isolated in the … A 17 th century B 18 th century C 19 th century D 20 th century 1

Nucleic acids were first isolated in the … 1 Friedrich Miescher

The major form of DNA in cells is … A A-DNA B B-DNA C C-DNA D Z-DNA 2

The major form of DNA in cells is … 2

A A-DNA B B-DNA C C-DNA D Z-DNA 3 Which form of DNA is shown here ?

3

Most RNA in cells is … A messenger RNA B transfer RNA C micro RNA D ribosomal RNA 4

Most RNA in cells is … 4

Transcriptomes consist of … A messenger RNA B transfer RNA C ribosomal RNA D all of the above 5

Transcriptomes consist of … 5

The genetic code A consists of 62 codons B has 3 stop codons C is used in transcription D specifies 25 amino acids 6

The genetic code 6

Restriction endonucleases A are located in the nucleus of cells B are sequence-specific C were discovered in the 1980s D are present in bacteria and fungi 7

Restriction endonucleases 7

Which compound is not found in nucleic acids? A Pyridines B Purines C Phosphate D Ribose 8

Which compound is not found in nucleic acids? 8 PyridinePyrimidine

The secondary structure of proteins is stabilized by … A Ionic bonds B Hydrogen bonds C Disulfide bonds D Hydrophobic interactions 9

The secondary structure of proteins is stabilized by … 9

DNA polymerases A synthesize DNA in 3’ -> 5’ direction B require a primer to initiate DNA synthesis C require ATP D bind only to DNA 10

DNA polymerases 10

The human nuclear genome … A is split into 24 chromosomes B Is the largest genome known to date C Contains about 2 billion bp D Contains about 40,000 genes 11

The human nuclear genome … 11 Human female karyogram

Reverse transcriptases A Synthesize RNA B Use DNA as template C Are viral enzymes D Are present in all eukaryotic cells 12

Reverse transcriptases 12

ORF scanning A is used to find intron sequences B is used to find functional genes C is used to identify protein-coding genes D is used to find intergenic sequences 13

ORF scanning 13

A codon bias … A is present in intergenic regions of a genome B is used to find protein- coding genes C is only present in eukaryotic genomes D has been found for all codons 14

A codon bias … 14

Expression of genes can be analyzed by A northern analysis B Southern analysis C Comparative genomics D PCR 15

Expression of genes can be analyzed by 15

An alpha-helix A is a DNA structure B is a protein structure C winds to the left D is stabilized by base stacking 16

An alpha-helix 16

ß-sheets are stabilized by A hydrophobic bonds B ionic bonds C hydrogen bonds D all of the above 17

ß-sheets are stabilized by 17