WEL COME

FIELD DETERMINATION, PRESERVATION TECHNIQUES

LABORATORY PREPARATION FOR SAMPLING SAMPLERS SAMPLE CONTAINERS REAGENT SOLUTIONS INSTRUMENTS FIELD KIT GLASSWARES

CHECK LIST FOR COLLECTION OF SAMPLE SAMPLING TOUR PROGRAMME AREA MAP ICE BOX FILLED WITH ICE DO SAMPLER BOD BOTTLE SAMPLE CONTAINERS SAMPLE PRESERVATIVES

CHECK LIST FOR COLLECTION OF SAMPLE TISSUE PAPERS SAMPLE ID FORMS FIELD NOTE BOOK SOAP AND TOWEL DRINKING WATER TRANSPORT ARRANGEMENT SAMPLING SITE LOCATION MAP WEIGHTED BOTTLE SAMPLER

CHECK LIST FOR COLLECTION OF SAMPLE ROPE SAMPLE CONTAINERS DO FIXING AND TITRATION CHEMICALS & GLASSWARES THERMOMETER FIELD KIT,AS REQUIRED LABELS FOR SAMPLE CONTAINERS PEN,PENCIL,MARKER,MATCH BOX,TORCH,KNIFE,GLOVES & EYE PROTECTION

Sample Collection RECOMMENDED SAMPLE COLLECTION DEVICES AND TYPES SR.NOPARAMETERDEVICECONTAINERQUANTITYPRESERVATION AT SITE 01D.O.DO SAMPLER BOD BOTTLE300 ml DO SETTING AT SITE 02GENERALPVC CAN 2000mlNONE 03COLIFORMSMPN BOTTLE STERILIZED BOTTLE 500ml COOLING AT 4 0 C

Sample Collection SAMPLE COLLECTION DEVICE AND TYPE 1)D.O.SAMPLER WITH 300 ml BOD BOTTLE ( TO AVOID AIRATION) 2) DEPTH INTEGRATING SAMPLER= D-74 ( DEPTH UPTO 4.72 m) 3) DEPTH INTEGRATING SAMPLER= P-74 ( DEPTH MORE THAN 4.72 m)

DO SAMPLER

20-30 CM BELOW THE SURFACE

SAMPLING FOR D.O. WATER LEVEL

D-74 SAMPLER FOR DEPTH UP TO 4.72 m

DEPTH INTEGRATING SAMPLER D-74 SALIENT FEATURES- CAST IRON STREAM – LINE BODY BOTTOM HINGED HEAD BOTTEL FITS IN TO THE CHAMBER TAIL VANES 4.8 mm NOZZEL NOZZELS-6.4mm,4.8mm AND 3.2mm AIR OUTLET VELOCITY – 2.4 m/s DEPTH – 4.72 m

P-61 SAMPLER FOR DEPTH MORE THAN 4.72 m

POINT INTEGRATING SAMPLER P-61 SALIENT FEATURES BRONZE CASTING – STREA LINED BOTTOM HINGED HEAD TAIL VANES 4.8mm NOZZEL PRESSURE EQUILISING CHAMBER ROTARY SOLENOID ACTIVATED VALVE 24 VOLTS CURRENT COLLECTING SAMPLES AT SELECTED POINT USED AS DEPTH INTEGRATING SAMPLERIN DEEP STREAM(DEPTH MORE THAN 4.72m)

USDH-48 SAMPLER USED WHERE :V x D < 1

USDH-48 SAMPLER SALIENT FEATURES LIGHT WEIGHT,SMALL & EASY TO OPERATE USED WHERE VELOCITY x DEPTH < 1 AIR OUTLET ALLUMINIUM CASTING WEIGHT 2 KG NOZZEL –6.30mm,4.80mm,3.15mm COLLECTING SAMPLES WITHIN 9 cm OF STREAM BED REQUIRED CABLE AND FISHWEIGHT

SELECTION OF ANALYTICAL PARAMETERS CONSIDERING WATER YEAR FROM JUNE TO MAY PARAMETERS TESTED IN LAB (AS PER TYPE OF LOCATION) BASELINE – Ist-30– Balance –20 TREND - Ist-30– Balance –20 TREND/FLUX -Ist-30– Balance –20 RESERVIOR/DAM -Ist-32– Balance –22

CLIPS

SAMPLING FOR DO

SAMPLING FOR BACTERIOLOGICAL SAMPLE

WATER TESTING KIT ( FIELD KIT)

FIELD TEST MEASUREMENT OF FIELD PARAMETERS COLOUR,ODOUR,TEMPARATURE,EC,pH,& DO ARE FIELD PARAMETERS AND ARE TO BE ANALYSED AS SOON AS SAMPLE IS COLLECTED MEASUREMENTS OF THESE PARAMETERS CAN BE MADE BY USING FIELD KITS.THIS IS BEST OPTION ANOTHER OPTION- NEARBY LEVEL-1 LAB D.O.SAMPLE MUST BE FIXED IMMIDIETLY AFTER COLLECTION OF SAMPLE TEMPARATURE MUST BE MEASURED AT THE SITE ONLY

FIELD TEST FILLING AN ID FORM

FIELD TEST COLOUR CODES 1)LIGHT BROWN 2)BROWN 3) DARK BROWN 4) LIGHT GREEN 5) GREEN 6) DARK GREEN 7) CLEAR 8) OTHER SPECIFY

FIELD TEST ODOUR CODE 1) ODOUR FREE 2) ROTTEN EGGS 3) BURNT SUGAR 4) SOAPY 5) FISHY 6) SEPTIC 7) AROMATIC 8) CHLORINIOUS 9) ALCOHOLIC 10)UNPLEASENT

FIELD TEST TEMPARATURE IN DEGREE CELCIOUS BY USING THERMOMETER OR TESTING KIT

FIELD TEST pH INDICATES CONCENTRATION OF H AND OH IONS INDICATES ACIDIC OR ALKALINE NATURE OF WATER WATER IS ACIDIC WHEN pH < 7 WATER IS ALKALINE WHEN pH > 7 THE pH of DISTILLED WATER IS 7

FIELD TEST MEASUREMENT OF pH LITMUS PAPER- ACIDIC – BLUE LITMUS PAPER TURNS IN TO RED ALKALINE – RED LITMUS PAPER TURNS IN TO BLUE NUTRAL – NO CHANGE IN ANY PAPER BY USING pH PAPER HAVING VALUES OF VARIOUS RANGES 4-11 (UNIVERSAL),1-14,1-10 etc.

FIELD TEST MEASUREMENT OF pH -DISC OF pH 10 ml TEST TUBE UNIVERSEL INDICATOR pH COMARATOR

FIELD TEST MEASUREMENT OF pH ELECTRICAL pH METER OR PENTYPE 1)REQUIRED STOCK SOLUTION 2)BUFFER SOLUTION OF 4,7, & 9.2 3)CALIBRATION OF INSTRUMENT BY USING BUFFER SOLUTION TO TAKE OBSERVATION

FIELD TEST DISSOLVED OXYGEN 1)GLASS WARES-D.O.BOTTLE,PIPPETES,BURRETS & CONICAL FLASK etc. 2)CHEMICALS( BUFFER SOLUTION) 1) MANGNESE SULPHATE, 2)ALKALI IODIDE AZIDE REAGENT 3) STARCH SOLUTION 4) SODIUM THIOSULPHATE 5) CONC.H2SO4

FIELD TEST ELECTRICAL CONDUCTIVITY EC AT FIELD – EC METER -CALIBRATION OF INSTRUMENT -RINSE CONDUCTIVITY CELL –0.01 M KCL MEASURE RESISTANCE, TEMPARATURE ADUST METER TO 1412 Micro mhos./cm COMPUTE THE CELL CONSTANT Kc RINSE CELL IN SAMPLE CALCULATE EC

FIELD DETERMINATION

MEASUREMENT OF D.O.

RESULT OF D.O. IS 4.74 MEASUREMENT OF D.O.

SAMPLE LABELLING SAMPLE CODE NUMBER DATE AND TIME OF SAMPLING SOURCE AND TYPE OF SAMPLE PRETREATMENT OR PRESERVATION ANY SPECIAL NOTE FOR ANALYST SAMPLERS NAME,etc.

HYDROLOGY PROJECT(SW) MAHARASHTRA WATER QUALITY LAB NASHIK Name of Location: ID No. River: Tal: Dist: Date of sampling: Sampling Time : Sample collected by: Sample received by: Sr.No. in Sample entry register: SAMPLE LABEL

SAMPLE PRESERVATION AND TRANSPORTATION

Water Quality Parameters Recommended Maximum Storage Times.

SAMPLE PRESERVATION AND TRANSPORTATION Water Quality Parameters Recommended Maximum Storage Times. Parameter Group Parameter Recommended Required GeneralTemperatureImmediate Suspended Solid7 days Conductivity28 days pHImmediate Dissolved Oxygen 8 hours Total Dissolved Solids7 days NutrientsAmmoniacal Nitrogen7 days 28 days Total Oxidized Nitrogen days Total Phosphorus---

Water Quality Parameters Recommended Maximum Storage Times. Organic Matter COD7 days28 days BOD6 hours48 hours Major IonsSodium6 months Potassium6 months Calcium6 months Magnesium6 months Carbonates and Bicarbonates 24 hours14 days Chloride28 days Sulphate28 days

Water Quality Parameters Recommended Maximum Storage Times. Other Inorganic Silica28 days Fluoride28 days Boron28 days6 months MetalsCadmium 3 6 months Mercury 3 28 days Zinc 3 6 months OrganicsPesticide (Indicator7 days Synthetic Detergents--- Organic Solvents--- Phenols days Micro bioTotal Coliforms 8 hours24 hours BiologicalChlorophyll ’a’30 days-----

SAMPLE TRANSPORTATION

WITHIN 24 HOURS TO CONCERN LABORATORY USE ICE BOX ( 4 0 C DARK) DURING TRANSPORTATION FOR MPN SAMPLES

ICE BOX ( 4 0 C DARK)

ANALYSIS OF SAMPLES IN THE LABORATORY

MPN TEST

CHEMICAL ANALYSIS

ANALYSIS FOR IONS BY AAS