Cellular Structures and Organelles Laura Fraser Cotlin, Ph.D. Fundamentals 1 – Dental/Optometry August 9, 2011.

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Presentation transcript:

Cellular Structures and Organelles Laura Fraser Cotlin, Ph.D. Fundamentals 1 – Dental/Optometry August 9, 2011

Inclusions Glycogen Lipofuscin Lipids Pigments -hemosiderin -melanin Crystals

Microtubules

Arrangement of MTs in flagellum or cilium – organized at the basal body

Structure of Actin filaments

Model for Cell Movement

Keratin Filaments in Epithelial Cells

Mechanical strength of filaments

The Cell Cycle

Introduction to Histology and Microscopic Techniques

A sense of scale between living tissue, cells and molecules

Resolving power of microscopes

BRIGHT-FIELD MICROSCOPYPHASE CONTRAST MICROSCOPY NOMARSKI DIFFERENTIAL- INTERFERENCE-CONTRAST MICROSCOPY DARK-FIELD MICROSCOPY Types of Light Microscopy

Comparison of Scanning (A), Interference Contrast (B), and Transmission Electron Microscopy (C)

A section of urine- collecting ducts in the kidney - stained with Hemotoxylin and Eosin (H &E)

Chicken fibroblasts infected with Trypanosoma cruzi -stained with H &E

Diagram of intestine with different staining

Intestinal Epithelium stained by Periodic Acid-Schiff (PAS)

Direct and Indirect Immunocytochemistry

Immunofluorescence Analysis

Reconstructing an image

Histology Virtual Lab