BARCODE OF LIFE DATA SYSTEMS (BOLD) Riadul Mannan Biodiversity Institute of Ontario

1 Architect, 2 Hardware Engineers 4 Programmers, 4 Data Managers BOLD SYSTEMS 450 CPUs, 100TB storage Generator power Remote backup

Barcode Records for Fungi, Protists, Plants and Animals 834.5Kspecimens, 69.6K species, 2.1K institutions : 5.1K users

Features of BOLD Quality Assessments Primer Registry Alignment Viewer/Editor Integrated Help Taxon ID Tree Distance Summary Sequence Composition Nearest Neighbour Summary Accumulation Curves Multi-Marker ID Engine Bibliography Support Web Service API Workflow *AnalyticsIntegration

Specimen Tissue Sample Extract DNA PCR Amplify Sequence Photograph Collection Data Web-Accessible Data and DNA Barcode The Analytical Chain

Specimen Taxonomy Identifier IDs Institution Collectors Desc. Lat/Lon Country Collection Lab Data Trace Primers Audit Trail Marker 1 DNA Marker 2 Markers 3 Caption Metadata Copyright Image/Video Literature BOLD Data Model

BOLD Multi-Marker Data Tag Cloud of markers Total Markers on BOLD : 45

Project Management

Data Management SpecimenSequence

Data Management

Primer Registry Total Primer – 633 Public – 325 Private – 308

Data Analysis & Visualization

Sequence Analysis - Taxon ID Tree

Alignment Browser Features:  Flexible labeling  Variability of positions  Mapping amino acids Future Features:  Highlighting diagnostic position  Mapping to secondary structures  Web based sequence editing

Identification System

Managing Lab Data - LIMS

LOCATING SAMPLES Owner Sample Type Sampleids Processids Keywords Microplate Label Sample Lookup

Controlling Liquid Handling Robots

PCR Success Report Gel image Sliced by system

Management And Monitoring

LIMS - Non Lab User

Data Publication