Glycolysis Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp Instructor: Kirill Popov

1.General metabolism of glucose 2.The reactions of glycolysis 3.Metabolism of hexoses other than glucose 4.Control of metabolic flux

Major ways in which glucose is metabolized in different tissues erythrocytesbrain muscleliver

Relative Changes in [ATP] and [AMP] When ATP Is Consumed Adenine nucleotide Concentration before ATP depletion (mM) Concentration after ATP depletion (mM) Relative change ATP % ADP1.0 0 AMP %

1.Glycolysis is a near universal pathway by which a glucose molecule is oxidized to two molecules of pyruvate, with energy conserved as ATP and NADH 2.All ten glycolytic enzymes are in cytosol, and all the intermediates are phosphorylated compounds of three or six carbons 3.In the preparatory phase of glycolysis, ATP is invested to convert glucose to fructose 1,6-bisphosphate; fructose 1,6-bisphosphate is than broken to yield two molecules of triose phosphate 4.In the payoff phase, each of the two molecules of glyceraldehyde 3- phosphate undergoes oxidation; the energy of this oxidation is conserved in the formation of NADH and ATP 5.Glycolysis is tightly regulated in coordination with other energy-yielding pathways