David Sampson School of Electrical, Electronic, & Computer Engineering

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Engineering capabilities: Optical engineering Photonics – optical fibre technology Building (portable) instruments Clinical measurements Engineering capabilities: Optical engineering Photonics – optical fibre technology Building (portable) instruments Clinical measurements Engineering capabilities: Optical engineering Photonics – optical fibre technology Building (portable) instruments Clinical measurements Engineering capabilities: Optical engineering Photonics – optical fibre technology Building (portable) instruments Clinical measurements

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Science capabilities: Optical coherence tomography Tissue optics – light scattering In vivo imaging Human upper airway Skin cancer – melanoma Two-photon microscopy Gross morphology In vivo, real-time Few millimetre depths Courtesy of M. Stante, P. Cappugi, University of Florence

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Science capabilities: Optical coherence tomography Tissue optics – light scattering In vivo imaging Human upper airway Skin cancer – melanoma Two-photon microscopy Mie scattering

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Science capabilities: Optical coherence tomography Tissue optics – light scattering In vivo imaging Human upper airway Skin cancer – melanoma Two-photon microscopy Optical coherence tomography Confocal reflectance microscopy Two-photon autofluorescence microscopy Arch. Dermatol. 137, , 2001 J. Biomed. Opt. 8(3), 432–439, 2003 Opt. Lett. 28, , 2003

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Science capabilities: Optical coherence tomography Tissue optics – light scattering In vivo imaging Human upper airway Skin cancer – melanoma Two-photon microscopy Oesophagus External nares Nasal cavity Nasopharynx Hypopharynx Orophayrnx Sleep apnoea Transverse section as probe is scanned along airway

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Science capabilities: Optical coherence tomography Tissue optics – light scattering In vivo imaging Human upper airway Skin cancer – melanoma Two-photon microscopy Melanoma in-situ

FABLS, Macquarie University, 21 February 2005OBEL Capabilities Two-photon autofluorescence microscopy Depth Phosphate- buffered saline Optical clearing agent Contrast/resolution/penetration in thick samples – all methods limited by scattering Can you reduce scattering by a biocompatible optical clearing agent? Investigate optical clearing agents – mechanisms, dynamics, specificity What else can we do to enhance thick tissue/in vivo imaging? Realise an in vivo instrument and establish what can it do?

FABLS, Macquarie University, 21 February 2005OBEL Capabilities FABLS – what would make a difference? Access to life sciences expertise –make possible interdisciplinary research –solve the right problems Attract more graduate students Training opportunities for researchers –in labs –high-level postgrad lectures/courses