M ETABOLIC ENGINEERING OF ALKALOIDS PRODUCTION IN PLANT Juan Pan Department of Plant Pathology.

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Presentation transcript:

M ETABOLIC ENGINEERING OF ALKALOIDS PRODUCTION IN PLANT Juan Pan Department of Plant Pathology

OUTLINE alkaloids biosynthesis in plant metabolic engineering summary

A LKALOIDS - N-containing small molecule - Amino acid derivative - Secondary metabolites - Defense in plant - Pharmaceutical uses

Alkaloid biosynthesis In plant - monoterpene indole alkaloid (MIA) Annu. Rev. Plant Biol :735-69

MIA biosynthesis scheme

Alkaloid biosynthesis in plant - benzylisoquinoline alkaloids (BIAs) tyrosine (S)-norcoclaurine Nature chemical biology, 2009, 5 (5): dopamine 4-hydroxyphenyl acetaldehyde (S)-reticuline

Alkaloid biosynthesis in plant - tropane alkaloids

Alkaloid biosynthesis in plant - purine alkaloids

Metabolic engineering of alkaloid biosynthesis - Overexpressing rate- limiting genes - Gene silencing to block pathway - Regulatory element

H6H PMT and H6H overexpression increased scopolamine production Proc Natl Acad Sci USA 101:

tyrosine (S)-norcoclaurine dopamine 4-hydroxyphenyl acetaldehyde (S)-reticuline COR Silencing COR blocked morphine biosynthetic pathway Nat Biotechnol 22:

Plant Biotechnology Journal (2008) 6, pp. 160–175 Exogenous regulatory factors increased alkaloids production in poppy

S UMMARY - Overexpression of rate-limiting enzyme for higher alkaloid production - Gene silencing to block certain pathway - Manipulating regulatory elements to increase alkaloid production - Combination of several methods