Suplemental Data Figure S1. Alignment of deduced amino acid sequence of CaGA2ox1 and other GA2ox homologs from the pepper EST database. Dark shading indicates amino acid residues that are conserved in CaGA2ox1. The asterisks indicate amino acid residues presumed to bind Fe at the active site. The closed triangles show the putative 2-oxoglutarate binding site.

CaActin Cadhn CaGA2ox1 100μM ABA H2OH2O H2OH2O Figure S2. Expression level of the CaGA2ox1 gene in pepper plants inoculated with a non-host bacterial pathogen, Xag8ra, was detected by RT-PCR The CaGA2ox1 transcripts in pepper plants treated with ABA and wound. The expression of Cadhn in ABA-treated leaves were monitored as positive controls MgCl 2 Xag8ra CaActin CaPR1 CaGA2ox Wound CaActin CaGA2ox1

pET pET:CaGA2ox1 pGEX kDa M kDa CaGA2ox1 SoGA2ox GST U I U I α-His ab pGEX:SoGA2ox Figure S3. Expression of recombinant CaGA2ox1 protein in BL21 pLysS. The recombinant fusion protein CaGA2ox1 was detected by western blot analysis with an anti-His antibody. U: uninduced, I: IPTG-induced.

CaPR1CaACCox Relative mRNA 6h 12h 6h 12h CaGA2ox1 CaKS25000C15cacn18210 MgCl 2 Xag8ra Figure S4. Expression patterns of GA-responsive genes and several defense-related genes in pepper plants using Q-RT-PCR analysis. Total RNA was extracted from leaf tissues of pepper plants at 6 h and 12 h after MgCl 2 or Xag8ra inoculation, respectively. CaPR4 6h 12h

PrimersSequences CaGA2ox1 FGTCTAATATGGTAGTGGCAACTC CaGA2ox1 RGGTAGCTAAGACTGCTAAGTACTGC cacn18210 FAAGAGCAACCATGGTGATCTTGTC cacn18210 RAATTCGGAGTTTGTCGATACGAGG CaKS25002C15 FACTACCCACCATATTGTAGTGATG CaKS25002C15 RCCCTATGCTTTACACTCTTAAACC CaPR1 F ACTTGCAATTATGATCCACC CaPR1 R ACTCCAGTTACTGCACCATT Cadhn F AGTGATCATTCTTTGCTTTATTCTTAC Cadhn R AACATTCATTCCCATGCTATC CaActin FTTGGACTCTGGTGATGGTGTG CaActin RAACATGGAGCCACCACTG Table S1. Primers used in a RT-PCR analysis.

PrimersSequences CaGA2ox1 FCGTAAGTGCAGTTAGGAAGCTGGC CaGA2ox1 RGCTCAGATGATGGGTCCCAATGAT CaPR1 FCAAGCCCAAAATTCTCCCCAAGA CaPR1 RTTGCATCCTGCAGTCTCCAATTC CaGA3ox FTAAATTCTTACCCGGCTTGTCCG CaGA3ox RTTAACAACTAACGCGCCACGAAG CaGA20ox FGCGATACATTTATGGCGCTTTCA CaGA20ox RCATGTGAGGTCGGGATACATTCG CaDhn FGACAAGTTGCCAGGTGGAGGAC CaDhn RCCTTCTTCTCCTTCCCCTCCG Cacn18210 FCCGCTGACCCTTTCGGCTAT Cacn18210 RATTTACCGCAGCCCGGATGT CaKS25002C15 FCAGGTGATGACAAATGGGAGGTTT CaKS25002C15 R CAAGCTGTCTTGATCCCCTTTCA CaACCox FGCCCCAAACCCGATTTGATT CaACCox RTGGAGTGGCGCATAGGAGGA Table S2. Primers Used for Q-RT-PCR.

GeneGene Bank Accession # (or EST #)Reference CaGA2ox1DQ465393in this paper cacn18210http://genepool.kribb.re.kr/new/ CaKS25002C15 CaGA3ox(CaKS13043D06) CaGA20ox(cacn5286) CaPR1AF053343Lee et al., 2004 CaActinGQ339766Lee et al., 2004 CaDhnAY225438Chung et al., 2003 Table S3. Pepper genes in this paper

REFERENCE Chung E, Kim SY, Yi SY, Choi D Capsicum annuum dehydrin, an osmotic-stress gene in hot pepper plants. Mol Cells 15: