Ivermectin Effects on Caenorhabditis elegans (C.elegans)

Slides:

Advertisements

Similar presentations

By: John Lee, Jon Fuller, and Vicky Hung

Advertisements

THE WORM Caenorhabditis elegans as a model organism.

Lifespan of C. elegans Separation of transgenic animals from nontransgenic animals Isolation of first-day adult worms Placement of first day adult worms.

Mechanisms of Anthelmintic Resistance

In vitro susceptibility of Acanthamoeba to antifungal drugs Bascom Palmer Eye Institute University of Miami - Miller School of Medicine A. Iovieno, MD;

Chapter 13 Genetic Control of Development Jones and Bartlett Publishers © 2005.

Onchocerciasis (River Blindness). River Blindness, a parasitic disease, is the second leading infectious cause of blindness.

I.INTRODUCTION Huntingtin associated protein 1 (HAP1) is believed to play a role in organelle trafficking and to interact with inositol-(1,4,5) triphosphate.

Soil-transmitted Helminthes Yemeng Lu. Overview Infectious Agents Infectious Agents Prevalence Prevalence Transmission Transmission Health Problems Health.

Mining the Haemonchus contortus genome for new anthelmintic targets Salma Siddiqui Forrestor Laboratory.

Comparison of the Diagnostic Value of the Standard Tube Agglutination Test and the ELISA IgG and IgM in Patients with Brucellosis Presented by Dr. Md.

Bacterial Mutation Analysis. The Bio Part What we want to do… Determine the rate of mutations in plasmids pBR325 CCTG 36 and pBR325 CCTG 58 in strains.

Antioxidants & Obesity in C. elegans Manhasset Science Research Madelyn Sayed.

Optogenetics Use of optics & Use of genetics

Treatment of Scabies: Permethrin vs. Ivermectin A04 義大醫院王柏欣, 陳奕峰, 詹子慶.

Human Behavior Adel Arif Al-Mudaifa Mrs.Timm English 12-B March 4 th Adel Arif Al-Mudaifa.

Greater Understanding of Lipids in Caenorhabditis elegans Exposed to Oxygen Deprivation Padilla Lab, Department of Biological Sciences Jemma Alarcón TAMSter.

Temporal Regulation of Cell Development: The Heterochronic Gene Pathway in Caenorhabditis elegans David Cooper, Department of Biology, York College of.

© Copyright 2009 by the American Association for Clinical Chemistry Nonfasting Lipids, Lipoproteins, and Apolipoproteins in Individuals With and Without.

Onchocerciasis (River Blindness)

Nematoda. Pseudocoelomates Common Characteristics Pseudocoel –Mesoderm muscle lined ectoderm Complete digestive tract Organs are within pseudocoel Syncytial.

Department of Kinesiology and Applied Physiology Nobel Prize in Physiology or Medicine William Campbell, Satoshi Omura "for their discoveries concerning.

Gene Preference in Maple Syrup Urine Disease Mary M. Nellis, Dean J. Danner The American Journal of Human Genetics Volume 68, Issue 1, Pages (January.

Lab 28 Goals and Objectives: Read results Exercise 31: Lethal Effect of UV Light Exercise 33: Effectiveness of Alcohol as an Antiseptic Exercise 36: Effectiveness.

Chapter 7 The Environment and Health. Table 7.0.T01: Key Links Between Environmental Health and the MDGs Modified from Millennium Development Goals.,

Genetic Variation of Renibacterium salmoninarum genes in infected salmonids Jeffrey Burnett HHMI Summer Investigator Dr. Dan Rockey Laboratory Biomedical.

Genetics Lab Dr. Jeff Young Office Hours: BI 412 MWT am or by appointment Lecture: T 11 – 11:50 pm in ES410 Lab: WF 1:30 - 4:30.

The Aschelminthes Phylum Nematoda. General Information The term “Aschelminthes” refers to a large group of about ten different phyla of soft- bodied worms.

Today’s Goals Describe the advantages of C. elegans as a model organism Discuss the life cycle of the nematode Safely and effectively culture a population.

Introduction to C. elegans Ms. Gaynor Honors Genetics

DNA Technology Biology 6(H). Learning Objectives Describe common DNA technology techniques Identify how each technique is used to study or manipulate.

Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)

Small interfering ribonucleic acids (siRNA’s) are double stranded RNA molecules used to post transcriptionally silence genes by binding to specific mRNA.

FIGURE 1-2 CARDIOVASCULAR DISEASE DEATHS AS A PERCENTAGE OF ALL DEATHS IN EACH REGION AND TOTAL REGIONAL POPULATION, (DATA FROM MATHERS CD, LOPEZ.

By Hannah Pedder, John Stafford, and Brooke Smith

C. elegans as a model organism

Prepared by : Nada H. Lubbad

Introduction and Purpose References and Acknowledgements

Ecdysozoa-Molting Animals Ecdysis- “an escape” -molting of exoskeleton or cuticle Phyla- Nematoda and Arthropoda.

FINDing NEMAtodes: C. elegans an Affordable and Interesting Model Organism Susan Groff Middle College High Santa Ana College

PREVALENCE OF SALMONELLA TYPHI AND PARATYPHI IN URINE CULTURES IN KARACHI Farhan E Abdullah, Syed Bilal Tanvir, Ali Zain Syed, Muhammed Fawwaz Haq Dr Essa’s.

Sample Title First Name, Last Name, Degree

Sample Title First Name, Last Name, Degree

Do Levels of EPA in C. elegans Alter Transcriptional Regulation of NPR-1 By: Oluchi ChIgbu.

The Short Sightedness and its Pitfalls

Genetics Lab Dr. Jeff Young

Non-coding RNA April 11, 2018.

Do Higher Levels of EPA in C. elegans Affect NPR-1’s regulation of AFT? By; Oluchi Chigbu.

Surviving Atmospheric Spacecraft Breakup

PSEUDOCOELOMATES PHYLUM NEMATODA PHYLUM ROTIFERA.

Genetics Lab Dr. Jeff Young

Genetics Lab Dr. Jeff Young

Today’s Goals Describe the advantages of C. elegans as a model organism Discuss the life cycle of the nematode Safely and effectively culture a population.

Unsegmented Worms.

Volume 78, Issue 5, Pages (June 2013)

Nature vs. Nurture: Examining the Impact of a Manipulated Environment

Mutations in Ppa-tph-1 reduce predatory feeding behavior.

C. elegans Behavior.

Caenorhabditis elegans is a model host for Salmonella typhimurium

Volume 23, Issue 21, Pages (November 2013)

Sexually Transmitted Diseases

Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx Young-jai You, Jeongho Kim, Melanie Cobb,

Volume 134, Issue 2, Pages (July 2008)

Virulence genotype and nematode-killing properties of extra-intestinal Escherichia coli producing CTX-M β-lactamases J.-P. Lavigne, A.-B. Blanc-Potard,

Chapter 12-1 DNA Part 1.

Unsegmented Worms.

Adaptive Capacity to Bacterial Diet Modulates Aging in C. elegans

Clade Ecdysozoa-Molting Animals Ecdysis- “an escape” -molting of exoskeleton or cuticle Phyla- Nematoda and Arthropoda.

Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans Alejandro Aballay, Peter Yorgey,

Presentation transcript:

Ivermectin Effects on Caenorhabditis elegans (C.elegans) Moyosore Olagoke

Background Information 1.Anti-parasitic Medication Strongyloides stercoralis Onchocerciasis volvulus Pubic lice infestation Scabies Wuchereria bancrofti http://www.pnas.org/cgi/content/full/97/6/2674/F2 2 Mechanism of action Bind to GABA Channel Hyperpolarization by ↑Cl Permeability C95H146O28

Background Information Phylum Nematoda Feed on OP50 bacteria Pseudocoelomates Thrashing motion (contraction) Female deposit 100,000 or more egg/day Most individuals are Hermaphrodites Life Cycle : >12hours Caenorhabditis elegans

Research Purpose :To determine the effect of Ivermectin on wild type (C.elegans) Plan to extend strain Complications Method of introduction Drug Test Resistance strain

Ivermectin response to OP50 bacteria Ivermectin has no effect on OP-50 bacteria Avermectin is isolated from strepto Avermectins

Drug Introduction Ivermectin→ Agar Sample calculation Stock 1- 0.1g/1000цl in 100% DMSO Stock 2- 10цl of stock 1 in 9.99ml 1%DMSO 15цl of stock 2 in 8цl Agar 10цg/цl x 15цl= 150цg 150 цg/8цl = 62.5цg/цl Ivermectin→ Agar

Wild type C.elegans

Ivermectin: Wildtype worm 62.5цg/цl Fewer numbers No apparent movement Worm appears transparent 18.75цg/цl Same conclusion Movement is perceived (~3 worms)

Comparison Control Ivermectin

Touch insensitive=Tapping

Future Analysis Growth Assay: Count the number of gravid adult Reflects Pharyngeal function Mechanosensation: Touch sensitivity Neurological Response Motility assay: Number of worm still thrashing Locomotion

References Patient Drug Information From MedMaster. Maryland: 2007. American Society of, Health-System Pharmacists Inc. Patient Drug Information From MedMaster. Maryland: 2007. Jamison, Dean T., and Joel G. Breman. Disease Control Priorities in Developing Countries. 2nd. New York: Oxford University Press, 1993. John Keane and Leon Avery Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genes. Genetics. 2003 May; 164(1): 153–162. PMCID: PMC1462566 Griffiths, Anthony J.F., and Jeffery H. Miller. HumanGenetics. 7th ed. New York: W.H. Freeman,1996. Hart, Anne C. "Behavior." Worm Book July 3 2006. 04 April 2008 <http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=wormbook. capter.7133>.