Producing drugs using genetically modified organisms

Genetic Modification Techniques 1. Selective Breeding 2.Genetic Engineering aka. Recombinant DNA technology

Genetic Engineering Process 1.Extraction of desired gene from host organism 2.Gene inserted into vector for transportation into new organism

Gene Extraction Process 1. Extracting gene from host cell chromosomes 2. Extracting mRNA from host cell

Production of Gene From mRNA (human insulin) 1.mRNA coding for insulin production removed from pancreas 2.Complementary DNA (cDNA) strand synthesised (reverse transcriptase) 3.Second DNA strand synthesised on cDNA base (DNA polymerase)

GM Microorganisms: Treating Diabetes Using Modified E-Coli 1.Human genes for insulin transferred to mutant form of E-Coli 2.Modified E-Coli cultured in fermenter 3.E-Coli secretes insulin which is extracted and used in treating diabetes

GM animals Manipulating genes in eukaryotes is more difficult than in prokaryotes due to: 1.Plasmids cannot be used to introduce new genes 2.Eukaryotes are diploid 3.Transcription of DNA to mRNA more complex 4.Gene expression process less understood

Introducing genes into Eukaryotic Cells 1.Injection 2.Bombardment 3.Electroporation

Treating emphysema using modified sheep Emphysema is the break down of the inner walls of the alveoli Caused by lack of AAT protein Sheep modified with human AAT genes so that they produce AAT rich milk

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GM Plants: Vaccines Antigen from animal pathogen added to plant virus surface Plant virus used as vaccine, triggers creation of antibodies against antigen Less associated safety risk as chosen plant virus cannot harm animals

Environmental and Ethical Issues of Genetic Engineering