Packard BioChip Technologies PerkinElmer Life Sciences 1 Dr. Steve Hawkins Technology Manager The GeneTAC TM G 3.

Slides:



Advertisements
Similar presentations
A Little More Advanced Biotechnology Tools
Advertisements

Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University.
Recombinant DNA Technology
MicroArray Image Analysis
MATLAB’s extensive, device-independent plotting capabilities are one of its most powerful features. They make it very easy to plot any data at any time.
Microarray Simultaneously determining the abundance of multiple(100s-10,000s) transcripts.
Image Quantitation in Microarray Analysis More tomorrow...
TIGR Spotfinder: a tool for microarray image processing
Introduction to DNA Microarray Technology Steen Knudsen, April 2005.
Introduction to DNA Microarrays Todd Lowe BME 88a March 11, 2003.
Agarose Gel Electrophoresis and Southern Transfer.
Microarray analysis Golan Yona ( original version by David Lin )
Figure 1: (A) A microarray may contain thousands of ‘spots’. Each spot contains many copies of the same DNA sequence that uniquely represents a gene from.
Robots for Printing Microarrays Jianping Zhou Robot 2003 Spring.
Microarray Technology Types Normalization Microarray Technology Microarray: –New Technology (first paper: 1995) Allows study of thousands of genes at.
Introduce to Microarray
Scanning and image analysis Scanning -Dyes -Confocal scanner -CCD scanner Image File Formats Image analysis -Locating the spots -Segmentation -Evaluating.
EPOCH 1000 File Management Data Logging and Reporting
Introduction to Lab Ex. 19: Enumeration of Bacteria
Molecular Biology Dr. Chaim Wachtel April 4, 2013.
Output devices. There is no point in having a computer that can do wonderful things unless it can tell you the results of what it has been doing. This.
Image Quantitation in Microarray Analysis More tomorrow...
CDNA Microarrays Neil Lawrence. Schedule Today: Introduction and Background 18 th AprilIntroduction and Background 25 th AprilcDNA Mircoarrays 2 nd MayNo.
© SSER Ltd..
Chapter 5 Nucleic Acid Hybridization Assays A. Preparation of nucleic acid probes: 1. Labeling DNA & RNA - Nick Translation - Random primed DNA labeling.
Affymetrix vs. glass slide based arrays
Page 1 Mouse Genome CGH Microarray 44A. Page 2 Mouse Genome CGH Microarray Kit 44A Designed for CGH, Validated with samples of known aberrations Designed.
The following slides have been adapted from to be presented at the Follow-up course on Microarray Data Analysis.
Data Type 1: Microarrays
Library screening Heterologous and homologous gene probes Differential screening Expression library screening.
Microarray - Leukemia vs. normal GeneChip System.
Plasmids Continued Once we insert the plasmid into the bacteria how do we know its in the bacteria and has the the right gene in it?
ARK-Genomics: Centre for Comparative and Functional Genomics in Farm Animals Richard Talbot Roslin Institute and R(D)SVS University of Edinburgh Microarrays.
Microarrays and Gene Expression Analysis. 2 Gene Expression Data Microarray experiments Applications Data analysis Gene Expression Databases.
Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.
MICROARRAY TECHNOLOGY
Computational Biology and Bioinformatics Lab. Songhwan Hwang Functional Genomics DNA Microarray Technology.
Chapter 8 – Bacterial and Viral Genetic Systems
Digital imaging By : Alanoud Al Saleh. History: It started in 1960 by the National Aeronautics and Space Administration (NASA). The technology of digital.
Microarray Technology. Introduction Introduction –Microarrays are extremely powerful ways to analyze gene expression. –Using a microarray, it is possible.
Microarray (Gene Expression) DNA microarrays is a technology that can be used to measure changes in expression levels or to detect SNiPs Microarrays differ.
Microarray hybridization Usually comparative – Ratio between two samples Examples – Tumor vs. normal tissue – Drug treatment vs. no treatment – Embryo.
(1) Normalization of cDNA microarray data Methods, Vol. 31, no. 4, December 2003 Gordon K. Smyth and Terry Speed.
Digital imaging By : Alanoud Al Saleh. History: It started in 1960 by the National Aeronautics and Space Administration (NASA). The technology of digital.
K.B.H.POLYTECHNIC,MALEGAON CAMP, MALEGAON. Computer Hardware & Maintenance. S.Y.C.M/I.F Guided By :- Mr.K.S.Pawar. Lecturer in Computer Department.
DNA Technology & Genomics
DNA Microarray Overview and Application. Table of Contents Section One : Introduction Section Two : Microarray Technique Section Three : Types of DNA.
Transcriptome What is it - genome wide transcript abundance How do you obtain it - Arrays + MPSS What do you do with it when you have it - ?
Class Four Microbiology Quiz  Quiz Number One – Open Response and Identification  Microscope  Aseptic techniques  Culture transfer techniques.
Introduction to Oligonucleotide Microarray Technology
Advanced 2D Design Concepts V The basic fill options include uniform or solid, fountain or gradient, and pattern. The uniform fill is produced.
Microarray: An Introduction
Inoculating a Petri Dish Procedure for Dry and Wet Swab.
Green with envy?? Jelly fish “GFP” Transformed vertebrates.
Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)
Microarray Technology and Data Analysis Roy Williams PhD Sanford | Burnham Medical Research Institute.
Slide culture Dalia Kamal Eldien Practical NO(4).
Detecting DNA with DNA probes arrays. DNA sequences can be detected by DNA probes and arrays (= collection of microscopic DNA spots attached to a solid.
A Little More Advanced Biotechnology Tools
CHAPTER 20 PART 3: A LITTLE MORE ADVANCED BIOTECHNOLOGY TOOLS
The Basics of cDNA Microarray Technology
© SSER Ltd..
Introduction to cDNA Microarray Technology
A Little More Advanced Biotechnology Tools
A Little More Advanced Biotechnology Tools
A Little More Advanced Biotechnology Tools
A Little More Advanced Biotechnology Tools
Microarrays: biotechnology's discovery platform for functional genomics  Mark Schena, Renu A Heller, Thomas P Theriault, Ken Konrad, Eric Lachenmeier,
Data Type 1: Microarrays
Walk-away specimen processor (WASP).
Presentation transcript:

Packard BioChip Technologies PerkinElmer Life Sciences 1 Dr. Steve Hawkins Technology Manager The GeneTAC TM G 3

Packard BioChip Technologies PerkinElmer Life Sciences 2 Micro-arraying Library management Change the tool to change the function Gridding / replicating Picking Takes less than 10 minutes

Packard BioChip Technologies PerkinElmer Life Sciences 3 A common user interface A standard method definition style Simplicity

Packard BioChip Technologies PerkinElmer Life Sciences 4 GeneTAC G3 Library Management System A multifunctional tool combining effective genomic library generation and management with array production GeneTAC micro-arrayer G3 –up to 4800spots (10 x 10 x 48) (equivalent to oligo spots) –Spot size ~300 µm –32 or 48 solid pins –Positional accuracy 100 µm –Positional precision 10 µm –384 well plates –multi-functional capabilities

Packard BioChip Technologies PerkinElmer Life Sciences 5 The G3 uses a dip and print technique for solid pins GeneTAC G3

Packard BioChip Technologies PerkinElmer Life Sciences 6 Fast cycle time Optimised motion for reproducible transfer of liquids

Packard BioChip Technologies PerkinElmer Life Sciences 7 32 or 48 pin tool using solid Titanium pins Printing BioChips

Packard BioChip Technologies PerkinElmer Life Sciences 8 Slide holder Marker plate holdercleaning Marker genes are separate from the library

Packard BioChip Technologies PerkinElmer Life Sciences 9 Genes spotted: 1920 Probes: (1) Cy3 labeled cDNA probe prepared from Human Adult total RNA (2) Cy5 labeled cDNA probe generated from Human Fetal total RNA Marker:Q gene from Lambda Example

Packard BioChip Technologies PerkinElmer Life Sciences 10 Effective Probe DNA Delivery

Packard BioChip Technologies PerkinElmer Life Sciences 11 Tracability

Packard BioChip Technologies PerkinElmer Life Sciences 12 GeneMAP™ Mouse Clone Array GeneMAP™ Array Chips

Packard BioChip Technologies PerkinElmer Life Sciences 13 rapid and accurate Colony picking

Packard BioChip Technologies PerkinElmer Life Sciences 14 Can use 96 well, 384 well or agar plates Colony Picking

Packard BioChip Technologies PerkinElmer Life Sciences 15 Programmable number of ‘dips’ Clones arranged in known order and position Colony Picking

Packard BioChip Technologies PerkinElmer Life Sciences 16 Compatible plate formats Input –15cm Petri –10cm Petri –22cm Bio-Assay –10cm Bio-Assay –GS Culture dish Output –96 well plate –96 deep well –384 well plate –GS Culture dish

Packard BioChip Technologies PerkinElmer Life Sciences 17 Plate selection

Packard BioChip Technologies PerkinElmer Life Sciences 18 Plate selection Using GS Culture plates Using Bioassay input plates Using Round Petri dishes

Packard BioChip Technologies PerkinElmer Life Sciences 19 Brushing to remove agar Sonication to sterilise Sterilization

Packard BioChip Technologies PerkinElmer Life Sciences 20 Ethanol for final sterilisation Heat lamp to dry Sterilization

Packard BioChip Technologies PerkinElmer Life Sciences 21 define the time for each step using arrows Sterilization Protocol

Packard BioChip Technologies PerkinElmer Life Sciences 22 Imaging On-board CCD Camera –Optimised imaging –440,000 pixels on the chip –each pixel represents about 0.01 mm 2 –8 bit grey scale resolution –excellent blue / white discrimination

Packard BioChip Technologies PerkinElmer Life Sciences 23 The IP Settings window Example of the Grabbed Image Image processing - IP Edge detect Size and shape Circularity Colour

Packard BioChip Technologies PerkinElmer Life Sciences 24 Workflow Picking performance –Typical capacity up to 20,000 clones per 8 hour day up to 15,000 clones per 8 hour day with double inoculation –colonies between 0.5 and 4.0mm –accuracy better than 99% –select on basis of colour, size and shape

Packard BioChip Technologies PerkinElmer Life Sciences 25 Gridding onto Hybond-N+ nitrocellulose filter

Packard BioChip Technologies PerkinElmer Life Sciences 26 Keeping track custom patterns allow easy cross referencing of the hybridisation results to the corresponding 384-well plate without the use of ink gridding

Packard BioChip Technologies PerkinElmer Life Sciences 27 Replication Direct copy of either 96 or 384 well plates using the gridding tool Expand 1 x 384 well to 4 x 96 Contract 4 x 96 to 1 x384 Eliminate the effects of freeze-thaw cycles Fast and accurate copying of libraries

Packard BioChip Technologies PerkinElmer Life Sciences 28 Library management Selectively re-array individual elements from a library Addresses individual wells in any library plate Rapid set-up via standard spreadsheet Full report generated Optional autoloader for total plate management Replication

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.