Introduction to Arabidopsis Research

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Presentation transcript:

Introduction to Arabidopsis Research

Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood Examine examples of how Arabidopsis thaliana are used experimentally

Arabidopsis thaliana Small flowering plant Popular as a plant model organism Plant Bio and Genetics In the family of Brassicaceae Mustard family

A. thaliana Arabidopsis anatomy Arabidopsis Development www.greenomes.org Arabidopsis Development

As a model organism Most commonly used plant model Advantages: Like the fruit flies of the plant world Advantages: Small size - Max 5cm in diameter, 30cm tall Easy to cultivate - grown in small area Small genome (5 chromosomes, 157 million base pairs, 27,000 genes) Rapid development - about 6 weeks to make mature seed Can self-pollinate Can perform crosses between different strains Many progeny Easy to perform genetic engineering Can be transformed using Agrobacterium tumefaciens

Commonly Used to Study Biotechnology Applications Light sensing Flower development

Commonly used related plant Brassica rapa Wisconsin Fast Plants Can use in classroom VERY easily Several mutant strains available Book on how to work with them www.fastplants.org

Research Projects You may choose this organism for your research project You may choose C. elegans You may talk with me and choose something else

Resources TAIR PREP website Books in my office www.arabidopsis.org Free Mutant strains! Some have uncharacterized phenotypes in terms of their growth & dev. PREP website http://www.prep.biotech.vt.edu/expinfo/expinfo_anatomy.html Suggestions for Experiments Books in my office Brassica rapa - related plant -called “Wisconsin Fast Plants”

Example I am interested in investigating salt tolerance in arabidopsis mutants. My approach is to compare plants with different T-DNA insertions ability to tolerate various concentrations of salt solutions My Literature search should . . . ?

Our Arabidopsis lab Learn to cultivate Arabidopsis in both soil and on agar plates Choose 1 parameter to measure Record in lab notebook Prepare you for your independent projects

Each group of 3 Make 1 bottle of 100mls plant agar. Make it in a 200ml media bottle Label it: MS Agar Date Initials