(Power)Point me in The right direction in Lab Mark Walvoord University of Oklahoma ABLE 2008.

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(Power)Point me in The right direction in Lab Mark Walvoord University of Oklahoma ABLE 2008

Chicken Wing Microbiology: A lesson in food safety and microbioogical technique Mariëlle Hoefnagels Mark Walvoord ABLE 2005 Proceedings, Vol. 27:

Two main objectives: 1.Measure populations of bacteria on chicken wings 2.Design an experiment to test a treatment that might get rid of the bacteria Important lessons: Basic microbiology techniques: serial dilution, plate count, aseptic technique Food safety Experimental design (investigative) Label your plates & test tubes Make “wing washes” for two chicken wings (per group) Make serial dilution (through in test tubes) for each wing Inoculate agar plates (2 duplicate plates per tube) Clean UP After, put contaminated trash (gloves, chicken, used pipets) in autoclave bags Put “clean” trash (pipet wrappers, paper towels, etc.) in room’s trashcan Procedures Aseptic Technique: Overall goal: Avoid contaminating your solutions or anything sterile, including pipets, saline, and agar. For example: Wash your hands before you begin (& afterwards); Wipe down bench with Lysol before you begin; Discard any plates with existing colonies; Open containers/petri dishes only when necessary, and then only briefly; Never touch pipet tip to any surface except the solution you are pipetting; Use each pipet only once, then set aside Timeline Week 1: set up initial counts of bacteria on chicken wings Week 2: count bacteria from week 1; design and implement independent experiments Week 3: collect data from independent experiments; write report to Wing Nut Chicken Wing Co.

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