Calcium binding proteins Maria Tereshina Vsevolod Belousov

Calcium binding protein CaBP2 Results of CD search by BLAST reveal two Efh domains

Calcium binding protein CaBP2 Results of CD search by BLAST reveal two Efh domains, each consisting of ef-hand motifs

Stricture of typical ef-hand containing protein, calmodulin

+4Ca 2+

N-terminal domain of calmodulin with bound Ca 2+ Yellow- key positions in ef-hand, coordinating Ca 2+ (usually Asp & Glu)

N-terminal domain of calmodulin with bound Ca 2+ Yellow- key positions in ef-hand, coordinating Ca 2+ (usually Asp & Glu)

Calsequestrin - ER protein with negatively charged surface that binds Ca 2+ with low affinity

Crystal structure of calsequestrin. Red spacefill- Asp & Glu residues

C2 domains- Ca 2+ -dependent lipid binding folds

Structure of C2 domain from PKC Yellow- Asp residues coordinating Ca 2+

Novel Ca2+ binding domain in bacterial collagenases

Structure of Ca 2+ -binding domain of collagenase Conformation changes upon Ca 2+ load

Structure of Ca 2+ -binding domain of collagenase Conformation changes upon Ca 2+ load

CONCLUSION The diversity of calcium binding proteins is great!