ISAC and Component-resolved Diagnosis

Slides:

Advertisements

Similar presentations

Egg Allergy Important food allergy in children Starts early in life

Advertisements

The Carbon Farming Initiative and Agricultural Emissions This presentation was prepared by the University of Melbourne for the Regional Landcare Facilitator.

Peanut Allergy 10% of children are sensitized but only 1-2% have symptoms Usually life-long Symptoms range from local reactions to anaphylaxis Very small.

ImmunoCAP ISAC Customer days

Manaaki Tangata Taiao Hoki protecting people and their environment through science Specialist Science Solutions FOOD ALLERGEN TESTING Peter Cressey ESR.

Bischenberg, 23 rd September 2007The GA 2 LEN/EAACI Allergy School Effects of heat treatment and proteolytic enzymes on allergenicity Dr. Montserrat Fernández.

Common Food Sensitivities, Allergens, and Intolerances

Lipoproteins Metabolism and Clinical Implications Jack Blazyk.

Literature support Test chart for the use of ImmunoCAP ® Allergen components Suspicion of fruit allergy Risk for severe reactions?

Intro to Biochemistry Using the empty slides provided (Make sure you give each slide a title!!) 1. Define and give a visual of the following: P. 148 Atom.

Module Two Labeled Test Methods

Recombinant Weed Allergens Nicole Wopfner Christian Doppler Laboratory for Allergy Diagnosis and Therapy Department of Molecular Biology University of.

Soy Allergy Important protein source Sensitization frequency varies largely Prevalence of soy allergy % Symptoms –cuteaneous or gastrointestinal.

Literature support Test chart for the use of ImmunoCAP® Allergen components Suspicion of peanut allergy Risk for severe reactions?

Literature support Test chart for the use of ImmunoCAP® Allergen components Suspicion of egg allergy Risk for clinical reactions?

A Macromolecule Investigation. What Are Macromolecules? Monomer Polymer.

1 Microarrays for allergy - ImmunoCAP ISAC © - Phn Romy GADISSEUR, Prof. J-P. Chapelle, Phn. Biol. E. Cavalier 02/03/2009.

Allergen regulation in the future : what will be the place for recombinant allergens ? Jacqueline DAYAN-KENIGSBERG European Academy of Allergology and.

New technologies in allergology Workshop Ph.Biol. Romy GADISSEUR 16/10/2010.

San Diego, February 2010 – AAAS Annual Meeting 1 Food Allergies: The Enemy Within Krzysztof Maruszewski European Commission Joint Research Centre.

2.2 Properties of Water TEKS 4B, 9A The student is expected to: 4B investigate and explain cellular processes, including homeostasis, energy conversions,

Allergenicity assessment of genetically modified organisms (GMOs )

Microarrays for allergy

Director of Scientific Affairs

Pre-AP Chemistry: Unit 1: Test Review

Historical overview Pr G. Pauli Hôpitaux Universitaires de Strasbourg Bischenberg 21 septembre 2007.

Class 5 Immuno-assay with magnetic nanoparticle tags Gaster et al Nature Medicine 15:1327 (2009) Basic idea Giant magneto resistance (GMR) Sensor characterization.

Chapter 3 Unit Review 1. You have a 78 g sample of a compound of which 12.4 g is hydrogen. What is the % by mass of hydrogen in the sample?

Component-resolved-diagnosis and validation of an allergen-microarray technology. Romy Gadisseur 1, Jean-Paul Chapelle 1, Etienne Cavalier 1 1 : University.

Western blotting. Antibodies in the Immune System Structure: 2 heavy chains + 2 light chains Disulfide bonds 2 antigen binding sites Isotypes: IgG, IgM,

Chemistry Mrs. Algier Do Now: Explain the difference between an intensive and an extensive property.

Lipids. Mostly carbon and hydrogen Fats, oils, and waxes Can be used to store energy  Also, part of cell membrane, and waterproof coverings, steroids,

Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood Jenny Zhou and Anne Ye Fan, Vermesh, et.

Classiifying Mixtures Heterogeneous Components not uniform Examples: soup, salad, … Homogeneous Components are uniform Examples: tea, soda,… Phase: a.

AdvanSure ® for diagnosis of specific IgE Park, Jung-Won Allergy-Asthma Clinic, Severance Hospital.

ALERBLOT: Interest of 2D Immunoblot in diagnosis of wheat allergy

Suggested IgE ab testing of suspected soy allergic patients

Sensitization profile in a group of Spanish patients allergic to LTP

Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens Kirsten Beyer,

62 Patients with positive sIgE f13 or n n= 38 (f13), n=24 (f17)

Allergy diagnosis for inhalant immunotherapy

Laboratory Diagnosis of Human Allergic Disease R.G. Hamilton, Ph.D. D.ABMLI Professor of Medicine and Pathology Johns Hopkins University School of Medicine.

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Eosinophilic gastrointestinal disease suggestive of pathogenesis-related class 10 (PR- 10) protein allergy resolved after immunotherapy Liliane De Swert,

Margaretha A. Faber, MD, PhD, Athina L. Van Gasse, MD, Ine I

بعض النقاط التي تؤخذ في الحسبان عند تقييم الاستثمارات الزراعية

Macromolecule Review.

Test Outline : Matter – Properties & Changes (Chp 3)

Quadrupole time-of-flight mass spectrometry: A method to study the actual expression of allergen isoforms identified by PCR cloning Johannes P.F.G. Helsper,

Firdaus Hamid, MD, PhD, Serge A. Versteeg, BSc, Aprilianto E

Heimo Breiteneder, PhD, Christof Ebner, MD

Changes in matter Mixtures.

Analysis of serum IgE reactivity profiles with microarrayed allergens indicates absence of de novo IgE sensitizations in adults Christian Lupinek, MD,

Recombinant allergens Pru av 1 and Pru av 4 and a newly identified lipid transfer protein in the in vitro diagnosis of cherry allergy Stephan Scheurer,

Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta

Component Resolved Diagnostics

Quiescence of the E. coli CFT073 original clinical isolate on glucose plates. Quiescence of the E. coli CFT073 original clinical isolate on glucose plates.

Epicutaneous immunotherapy for peanut allergy modifies IgG4 responses to major peanut allergens Stef J. Koppelman, PhD, Aurélie Peillon, MSc, Wenceslas.

Kerstin Bauermeister, MSc, Barbara K

Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion Stephan.

Class I chitinases, the panallergens responsible for the latex-fruit syndrome, are induced by ethylene treatment and inactivated by heating Rosa Sánchez-Monge,

Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for.

Aim: Organic Compounds # 2 - Proteins

Carrot allergy: Double-blinded, placebo-controlled food challenge and identification of allergens Barbara K. Ballmer-Weber, MDa, Brunello Wüthrich, MDa,

Peanut oral immunotherapy decreases IgE to Ara h 2 and Ara h 6 but does not enhance sensitization to cross-reactive allergens Riikka Uotila, MD, Anna.

Microarray immunoassay: Association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes Wayne G. Shreffler, MD,

Sensitivity and specificity plots of human epididymis protein 4 (HE4) (A and B, respectively) and carbohydrate antigen 125 (CA-125) (C and D, respectively)

Biochemistry Practice Test #2

Presentation transcript:

ISAC and Component-resolved Diagnosis April 23, 2013 ISAC and Component-resolved Diagnosis Douwe de Boer, Chris Nieuwhof, Judith Bons en Paul Menheere

Ebo et al., University of Antwerp CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Allergy: clinical relevance  “in vitro” testing intra-allergenic heterogeneity allergen = mixture of allergen components Ebo et al., University of Antwerp Component-resolved Diagnosis (CRD) B A E C D

ISAC (Immuno Solid-phase Allergy Chip ) CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 RAST Single-analyte assay: single analysis of specific IgE directed against single allergens, single allergen components or mixtures of allergens classical ImmunoCAP assay RAST1 40 µL ISAC (Immuno Solid-phase Allergy Chip ) ImmunoCAP ISAC (16 spots per mm2) Multiplex assay: multiple analysis of specific IgE directed against allergen components in one single run ISAC112 30 µL

Component-resolved Diagnosis (CRD) CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Storage protein Storage protein intra-allergenic heterogeneity Storage protein Storage protein Profilin Storage protein Storage protein Pathogenesis Related-10 protein non-specific Lipid Transfer Protein Component-resolved Diagnosis (CRD)

Complications Detecting specific IgEs - 1 CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Complications Detecting specific IgEs - 1 intra-allergenic heterogeneity inter-allergenic homogeneity non-specific Lipid Transfer Proteins Ara h 9 Pathogenesis Related-10 proteins Ara h 8 Profilins Ara h 5 Pan-allergens

Component-resolved Diagnosis (CRD) CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Complications Detecting specific IgEs - 2 Component-resolved Diagnosis (CRD) intra-allergenic heterogeneity inter-allergenic homogeneity

Pan-allergens – risks and severity CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Pan-allergens – risks and severity initially CCD was considered to be important to explain cross-reactivity mainly “plant” derived pan-allergens Cross-reactive Carbohydrate Determinants specific glycan epitope

Profilins Cytoskeleton proteins Highly conserved proteins CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Profilins Cytoskeleton proteins Highly conserved proteins True pan-allergen (high degree of cross-reactivity) rMal d 4 rHev b 8 rPhl p 12 rBet v 2 rMer a 1 rAra h 5 rPru p 4

Pathogenesis Related-10 Protein (PR-10) CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Pathogenesis Related-10 Protein (PR-10) Large family and conserved proteins Heat and acid labile proteins Unknown function rMal d 1 rDau c 1 rPru p 1 rAra h 8 rCor a 1.01 rCor a 1.04 rApi g 1 rGly m 4 rBet v 1 rPru ar 1 rPyr c 1 rPru av 1

non-specific Lipid Transfer Protein (LTP) CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 non-specific Lipid Transfer Protein (LTP) Relatively conserved proteins Heat and acid stable protein Shuttle protein of lipids rCor a 8 rPru p 3 rAra h 9 rMal d 3 rZea m 4 rPru av 3

Storage Protein Proteins for storage of energy mainly in seeds CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Storage Protein Proteins for storage of energy mainly in seeds Relatively less conserved proteins pseudo-Pan-allergen (low degree of cross-reactivity) “Voorraad of opslag eiwitten” rBer e 1 rAra h 1 rAra h 2 rAra h 3 rAra h 6 rCor a 9 rSes i 3 nGly m 5 nGly m 6

Pan-allergen sensitization in Europe CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 Pan-allergen sensitization in Europe

ISAC and Component-resolved Diagnosis CRD and ISAC | De Boer, Nieuwhof, Bons and Menheere | April 23, 2013 ISAC and Component-resolved Diagnosis Conclusions and remarks Component-resolved Diagnosis can give insights in sensibilisations for separate allergen components intra-allergenic heterogeneity Besides separate allergen components groups of pan- allergens can be distinguished inter-allergenic homogeneity  Components and pan-allergens are being associated with risks  ISAC is a multiplex assay (an extensive number of components)  RAST is a single analyte assay (limited number of components)