The tricarboxylic acid (TCA) cycle Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp 563-591 Instructor:

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The tricarboxylic acid (TCA) cycle Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp Instructor: Kirill Popov

1.Metabolic sources of acetyl-CoA 2.Enzymes of the Citric Acid Cycle 3.Regulation of the Citric Acid Cycle 4.The amphibolic nature of the Citric Acid Cycle

Stoichiometry of Coenzyme Reduction and ATP Formation in the Aerobic Oxidation of Glucose via Glycolysis, Pyruvate Dehydrogenase Reaction, the Citric Acid Cycle, and Oxidative Phosphorylation Reaction Number of ATP or reduced coenzymes directly formed Number of ATP ultimately formed* Glucose → glucose 6-phosphate-1 ATP Fructose 6-phosphate → fructose 1,6-bisphosphate-1 ATP 2 Glyceraldehyde 3-phosphate → 2 1,3-bisphosphoglycerate2 NADH ,3-Bisphosphoglycerate → 2 3-phosphoglycerate2 ATP2 2 Phosphoenolpyruvate → 2 pyruvate2 ATP2 2 Pyruvate → 2 acetyl-CoA2 NADH5 2 Isocitrate → 2 α-ketoglutarate2 NADH5 2 α-Ketoglutarate → 2 succinyl-CoA2 NAD5 2 Succinyl-CoA → 2 succinate2 GTP2 2 Succinate → 2 fumarate2 FADH Malate → 2 oxaloacetate2 NADH5 Total30-32 *This is calculated as 2.5 ATP per NADH and 1.5 ATP per FADH 2. A negative value indicates consumption.

Anaplerotic Reactions ReactionTissue(s)/organism(s) Liver, kidney Heart, skeletal muscle Plants, yeast, bacteria Eukaryotes and prokaryotes