DEPARTMENT OF CELL BIOLOGY Head: Dr. Jacek Bigda, Associate Professor Research Staff: Dr. Małgorzata Doszczak Dr. Patrycja Koszałka Dr. Zuzanna Dobrzańska Ph.D. Students: Marcin Okrój Arkadiusz Pierzchalski
MAIN TOPIC Mechanism of action of tumor necrosis factor (TNF) in vitro & in vivo TEACHING cell biology, histology, immunology
DEPARTMENT OF CELL BIOLOGY research interests: mechanism of IL-1-induced cytotoxicity; intracellular signaling of tumor necrosis factor; mechanism of toxicity of the anti-angiogenic fumagillin derivative, TNP-470; mechanism of anti-tumor action of poly-RGD peptides; mechanism of accelerated growth of hamster melanoma following LPS application.
U 937 – human histiocytic lymphoma TNF-R55 F N TF N TF N T TNF-R75 F N T m F N T m F N T
Cycloheximide (CHX) U937 cell variants
U 937 M U 937 G U 937 ATCC
IL-1 cytotoxicity
control 4 h 2 h 0,5 h 1 h TNF [pg/ml] control CHX IL-1 IL-1/CHX IL-1 cytotoxicity
Isotype control Untreated IL-1/CHX
DEPARTMENT OF CELL BIOLOGY financial support: Analysis of mechanism regulating secretion of TNF following application of interleukin 1 and cycloheximide; State Committee for Scientific Research, Poland ( ); Mechanism of synergistic anti-tumor action of tumor necrosis factor (TNF) and angiogenesis inhibitor TNP-470; State Committee for Scientific Research, Poland ( ); Analysis of TNF sensitivity of variants of the U937 cell line; Intramural grant, Medical University of Gdańsk ( ); Modulation of tumor growth by means of cytokines; Core fund, Intercollegiate Faculty of Biotechnology UG-MUG;
DEPARTMENT OF CELL BIOLOGY selected previous grants: Significance of tumor necrosis factor and its receptors for growth and dissemination of Bomirski melanoma; State Committee for Scientific Research, Poland ( ); Immunomodulatory and anti-tumor activity of cloned and purified rat tumor necrosis factor; State Committee for Scientific Research, Poland ( ); Influence of receptors of tumor necrosis factor for apoptosis of cancer cells; State Committee for Scientific Research, Poland ( ); Mechanism of cytotoxic action of interleukin 1; State Committee for Scientific Research, Poland ( ); TNF gene polymorphism and humoral reactivity against p53 protein in patients suffering from non-small cell lung cancer; State Committee for Scientific Research, Poland( ).
Tri-City Central Animal Laboratory, Medical University of Gdańsk
Tri-CityCentral Animal Laboratory, Medical University of Gdańsk Tri-City Central Animal Laboratory, Medical University of Gdańsk