Protein Synthesis Yes, it is still chapter 3!.  It is the DNA in the nucleus that stores instructions for protein synthesis and this DNA is contained.

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Protein Synthesis Yes, it is still chapter 3!

 It is the DNA in the nucleus that stores instructions for protein synthesis and this DNA is contained in chromosomes.

 Human body cells contain 23 pairs of chromosomes.  Each chromosome contains DNA strands wrapped around special proteins called histones.

Gene  The functional unit of heredity.  Consists of all the triplets (3 bases that specify an amino acid) needed to produce a specific protein.

Protein Synthesis  Divided into transcription and translation. Transcription is the production of RNA from a single strand of DNA. Translation is the assembling of a protein by ribosomes, using information carried by RNA molecule.

Transcription

 Takes place inside of the nucleus.  A codon is 3 bases on the new RNA strand.  DNARNA GCGC CGCG AUAU TATA

Step 1 Begins when the enzyme RNA polymerase binds to the promoter of a gene.

Step 2 Enzymes promote the synthesis of a mRNA strand using nucleotides complementary to those in the gene.

Step 3 Continues until DNA stop signal is reached. The enzyme and mRNA detach and the complementary DNA strand reattaches.

Translation  Occurs in the ribosomes.  Process of assembling proteins from information carried by the mRNA molecule.

Step 1  Begins at the “start” codon of the mRNA.  Ribosome binds to the mRNA molecule near the codon at the beginning of the messenger strand.  Joined by the first tRNA which carries the amino acid methionine (UAC).

Step 2  Small and large ribosomal subunits interlock around mRNA strand.  Second tRNA arrives at the next binding site of the ribosome.

Step 3  Anticodon of second tRNA binds to the next mRNA codon.

Step 4  First amino acid is detached from tRNA and joined to the next amino acid by a peptide bond.  First tRNA detaches as next arrives and attaches to mRNA.

Step 5  Amino acid chain continues to elongate until the stop codon is reached.  Components separate.  As the chain of amino acids develops, it folds into the unique shape of a functional protein molecule.

DNAmRNA –codontRNA- anticodon Amino acid AAA TAT CCT GGT AGA ATT ATG

AnticodonAmino acid AAALysine AAUAsparagine ACUThreonine AGAArginine AUGMethionine AUUIsoluecine CAAGlutamine CAUHistidine CCUProline GAAGlutamic acid GAUAspartic acid GCUAlanine GGUGlycine GUUValine UAAStop codon UAUTyrosine UCUSerine UGGTryptophane UGUCysteine UUALeucine UUUPhenylalanine

1. Summarize in your own words the process of transcription. 2. Summarize in your own words the process of translation.

1. What is the function of DNA? 2. Suppose that a DNA segment has the following nucleotide sequence: CTC ATA CGA TTC AAG TTA. What is the nucleotide sequence that would be found in a complementary mRNA strand? 3. Summarize in your own words the process of transcription.