Erie’s Lab Zimeng Li

Content Questions Feasibility Problems and Solutions Video/Fluid AFM

Questions Sliding clamp model Improve image resolution Study orders in the pathway Differentiation of protein complex What else?

Feasibility Physical blockage AFM scanning speed

Known Problems and Solutions Water – constantly drying Injection of reactants Constant translational movement

Water – constantly drying Closed Fluid Cell Drop water

Injection of reactants Closed Fluid Cell Needle Injection

Constant translational movement

Glue Magnet Simultaneously move scan area

Video AFM Fluid AFM

Video AFM

Fluid AFM Samples still attached to surface Tips interacting with sample Cantilever drive frequency is coupled with fluid relaxation frequency

To-dos