(z=7) (z=7) (z=7) (z=7) MSMS/MS Ba 7702 Bc AH819 Bc Bc AH259 b y y b b 9 2+ b y y b b 9 2+ b y y b b 9 2+ b y y b b 9 2+ Supplementary Figure S-1 A

B C

MSMS/MS Bc CA Supplementary Figure S (z=7) (z=7) (z=7) b y y b b b y y b b b y y b b 9 2+ Bc CI Bc B06020

Supplementary Figure S-3 B. anthracis A

Supplementary Figure S-3 B. anthracis B

C Supplementary Figure S-3

B. anthracis D Supplementary Figure S-3

B. anthracis E Supplementary Figure S-3

B. anthracis F Supplementary Figure S-3

B. anthracis G Supplementary Figure S-3

B. anthracis H Supplementary Figure S-3

B. anthracis I Supplementary Figure S-3

B. anthracis J Supplementary Figure S-3 * * *

Supplementary Figure S-4 LVGGVAVIK ILDQSADK GTPDQEVIDQVLNVFK ARSTNKLAVPGAE SRSTNKLAVPGAE LVSLAEQQLGGFQK LVSLAEQQLGGYQK AKKAQASGASIQSTNASYGTE 2 min seg 1 seg 2 seg 3 seg 4seg 5

SASP-B (N-Ter peptide) SASP-B (C-Ter peptide) 30S ribosomal protein S10 SASP-gamma 60kDa Chaperonin Lipoprotein A B IS Mix 5 Bc IS Mix 5 Bc + 1 Ba (ratio 1:0.01) RT: 7.99 AA: RT: AA: RT: AA: RT: AA: RT: 8.13 AA: RT: AA: Supplementary Figure S-5

SASP-gamma IS  Sample  : 1 Ratio Ba : Bc mix 0.01 : 10.1 : 1 1 : 1 Supplementary Figure S : 10.1 : 1 1 : 1 A B