Microbiology Overview KWWOA April 09, 2014 Department for Environmental Protection Energy & Environment Cabinet.

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Presentation transcript:

Microbiology Overview KWWOA April 09, 2014 Department for Environmental Protection Energy & Environment Cabinet

Topics

o Proficiency Tests o Chain of Custody o Methods o Membrane Filtration

Proficiency Tests

o Drinking Water: WS Study SW sample for MPN o Wastewater: DMR-QA WP Study o KY Approved PT Providers List

Proficiency Tests

Revised reports will not be accepted. Photo: peoplesdaily.com

Chain of Custody

Client name & address Sampler name Sample location Collection date & time Sample type (grab/composite) Matrix (DW/WW) Field sample ID Laboratory sample ID Container type, & size/volume Number of containers Preservative (ice, etc.) Field data (pH, TRC, etc.) Requested analysis Reason for rejection or special remarks Signature, date & time for each transaction (relinquishing & receiving) Sample temperature upon arrival at lab

BARF is NOT a COC

Methods

Microbiology Methods MethodDrinking WaterWastewater Colilert E. coli Colilert-18 E. coli Fecal – 44.5 o C Colisure Coliscan mFC NA-MUGFecal mEndo EC-MUG mColiBlue-24 E. coli Modified mTEC E. coli

17 Colilert-18 P/A Colilert-18 pre-warming

18 Colilert-18 MPN

Membrane Filtration

Dilutions: Select appropriate volumes Fecal Coliform or E. coli colonies: 20-60* Total colonies: 200 *Use range as specified in particular method. Photo:

Membrane Filtration Blanks: Beginning of series End of series RECORD results of blanks

Membrane Filtration Calculating Results: Use dilution that is in range to determine result. If multiple plates within range, average those plate counts. If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments. Photos:

Membrane Filtration Calculating Results: If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments. WW TNTC = 60,000

Contact Information Tiffany Nolan Kentucky Department for Environmental Protection Division of Water 200 Fair Oaks Lane; 4 th Floor Frankfort, KY Phone: (502)

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