NA-MIC National Alliance for Medical Image Computing Measuring Alcohol and Stress Interactions with Structural and Perfusion MRI Chris.

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NA-MIC National Alliance for Medical Image Computing Measuring Alcohol and Stress Interactions with Structural and Perfusion MRI Chris Wyatt External Collaboration

National Alliance for Medical Image Computing Introduction Our goal is to use MRI to examine the effects of chronic alcohol self-administration on brain structure and function in monkeys. Team –Jim Daunais, Bob Kraft (WFUHS) –Xiaoxing Li, Vidya Rajagopalan, Chris Wyatt (VT) –Andriy Fedorov, Killian Pohl, Sandy Wells (NA-MIC)

National Alliance for Medical Image Computing Experimental Design Male vervet monkeys were chosen based on their initial cortisol response to novelty. Classified as: –high cortisol response (HC) –low cortisol response (LC) Groups then divided into EtOH or controls Classic 2 X 2 design: HC/controlLC/control HC/EtOHLC/EtOH

National Alliance for Medical Image Computing Experimental Protocol Training Water induction 0.5 g/kg induction 1.0 g/kg induction 1.5 g/kg induction 22 hour self- administration (2 drinks) (4 drinks)(6 drinks) Baseline structural and MRS Post-induction structural, MRS and perfusion Cynomolgus Monkey At The Panel (Courtesy Dr. Kathleen Grant, OHSU) Custom Head Coil Example T1w Image

National Alliance for Medical Image Computing NA-MIC Related Goal Implement and validate algorithms for: Size comparison of human, rhesus, and cynomolgus brain. brain extraction white-gray matter segmentation subcortical structure segmentation

National Alliance for Medical Image Computing Progress Report Version 1 of Vervet atlas complete Initial workflow implemented, documented in a tutorial Subcortical structures well segmented, issues with image artifacts and cortical segmentation All data is collected N = 10 subjects

National Alliance for Medical Image Computing Current Efforts Working on a Slicer only workflow to help with dissemination –BRAINSFit Module replacement for FSL-flirt Working on an improved atlas Segmenting second time point data Analysis of results Resubmission –continue structural –add functional imaging

National Alliance for Medical Image Computing Acknowledgements Measuring Alcohol and Stress Interactions with Structural and Perfusion MRI NIH NIAAA R01 AA (PI James Daunais) Early Stress & Alcoholism: Neurobiological Analysis NIH NIAAA U01AA (PI David Friedman) Team –Jim Daunais, Bob Kraft (WFUHS) –Xiaoxing Li, Vidya Rajagopalan, Chris Wyatt (VT) –Andriy Fedorov, Killian Pohl, Sandy Wells (NA-MIC)