Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Federal Centre of Breeding Research on Cultivated Plants Introduction of the work plan, timetable of deliverables Christoph U.Germeier Introduction of the work plan, timetable of deliverables Christoph U.Germeier

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Introduction to the project Project partners and co-sponsors Work packages Milestones and Deliverables in 2007/2008 some questions

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Project Partners P0:Federal Centre of Breeding Research on Cultivated Plants (BAZ), RCC Plant Genetic Resources, Quedlinburg, Germany 15 partners from 9 countries P1: Plant Breeding and Acclimatization Institute (IHAR), Radzikov, Poland P2: Svalöf Weibull AB Cereal Breeding Department, Svalöv Sweden P3: Federal Centre of Breeding Research on Cultivated Plants, Groß Lüsewitz, Germany P4: eurofins Analytik GmBH| Wiertz-Eggert-Jörissen, Hamburg, Germany P5: INRA Cereal Genetic Resources Centre, Clermont Ferrand, France P6: Institute for Cereal Research of Bergamo, Bergamo, Italy P7: Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden P8: Experimental Institute for Cereal Research of Fiorenzuola d’Arda, Fiorenzuola d’Arda, Italy P 9: Suceava Genebank, Suceava, Romania

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Project Partners P11:Jõgeva Plant Breeding Institute, Jögeva, Estonia 14 partners from 9 countries 3 Co-sponsors Peter Koelln KGaA, Elmshorn, Germany P10:IPGR “K.Malkov”, Sadovo, Bulgaria P12:IHAR, Laboratory of Quality Evaluation of Plant Materials, Radzikow, Poland P14: Agricultural Research Institute Kromeriz, Ltd., KromerizCzech Republic P15: Agrotest Fyto, Ltd., KromerizCzech Republic Emco Ltd., Praha, Czech Republic Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP), Bonn, Germany

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand Work packages Workpackages and Time Frame 1Project Management and CoordinationP0 (DEU) Project working collectionP1 (POL) Field experiments and sampling seeds for quality analysisP9 (ROM) Field experiments with artificial Fusarium inoculationP3 (DEU) Fusarium and mycotoxin analysisP15 (CZE) Analysis for protein and fat and mineralsP5 (FRA) Analysis for ß GlucanP6 (ITA) Analysis for AntioxidantsP7 (SWE) Analysis for cold toleranceP8 (ITA) Project documentation and internet portalP0 (DEU) LeaderPMDuration

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 WP10: Data Management P1P2P3P4P5P6P7 P8 P9P10P11P12P14P15 P6 WP8: Antioxidants NP-HPLC WP7: ß-Glucan/Carbohydrates Enzyme P2P6 WP2: Working Collection P0P1 P9 P10 P5 MultipliersProvide some germplasm P8P6 WP6: Protein and Fat NIRS P5 P11 WP1 Coordination P1 P0 P9 P5 P6 P3 P7 P15 P8 samples Kalibration Protein Fat P9P11 FIA Starch Fibre P12 AVA P7 WP5: Mycotoxin - Fusarium ELISA PCR P6P15P8 LC-MS/MS P4 WP3: Quality Field Experiments P11 P2P1P9 Nordic (N) E SW(S) E P10P5 S P8 WP4: Fusarium Field Experiments P3 (N) E-EuropeSE P9P14 S P8 seed WP9: Frost Tolerance S-Europe SE P9 Field- Test Climate C. P8

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) March March 2009 M1.3 Second project meeting and mid-term review (Quedlinburg), M1.4, D1.2 Second annual report to the Commission Question:Autumn 2008 for next meeting ok?: - End of WP1 (Multiplication of working collection) - After first field experiment year - Before onset of laboratory work March March 2008 M1.1 First project meeting M1.2, D1.1 First annual report to the Commission WP1: Management and Coordination

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP2: Project working collection Questions:- Methodology of seed testing of harvested material - Germination (plate test, soil test?, protocol) - Test of Fusarium infection? (protocol) - Consequences and thresholds? March March 2009 M2.5, M2.62nd 300 accessions + 10 standards (5 replications) multiplied M2.7, D2.5 Harvest germinability and infection tested and delivered March March 2008 M2.1 Accessions selected for use in the project M2.2, M2.31st 300 accessions + 10 standards (5 replications) multiplied M2.4, D2.4 Harvest germinability and infection tested and delivered to field partners

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP3: Field experiments and sampling seeds for quality analysis Questions:- Field design; suggestion: augmented block - Standards, how many? - Descriptors (IBPGR, UPOV, other?) - Plot size, sowing density, standardized? March March 2008 M3.1First project meeting, working collection approved, D3.1 List of descriptors agreed on; March March 2009 M3.2First evaluation year field experiments sown (300 accessions + five to ten standards in five replicdations). M3.3First evaluation year field experiments harvested. M3.4, D3.2First year samples distributed to analytical labs and observation results delivered to the project documentation.

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP3: Descriptors suggested in the proposal Questions: -Methods (protocols) for observations, measurements preferred where possible - Colour of lemma, - Color of kernel, - Days to heading and to maturity, - Crop height, - Lodging, - Panicle shape, - Number of sterile spikelets, - Occurrence of diseases, - Hull content (partners with dehulling equipment, e.g. P3), - Thousand kernel weight (200-kernel samples in triplicate), - Test weight.

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP4: Field experiments with artificial Fusarium inoculation Questions:- same as WP3 and additional: - Control treatment (not inoculated)? - Inoculum, inoculation method? March March 2008 M4.1First project meeting, working collection approved, D4.1 List of descriptors agreed on; March March 2009 M4.2First evaluation year field experiments sown (300 accessions + five to ten standards in five replications). M4.3First evaluation year field experiments harvested. M4.4, D4.2First year samples distributed to analytical labs and observation results delivered to the project documentation.

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP4: Descriptors suggested in the proposal Questions: -Methods (protocols) for observations, measurements prefered where possible - Panicle emergence (heading) date - Leaf index - Crop height - Plot density (number of panicles per m row) - Panicle shape - Panicle length - Number of kernels per spikelet

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP5-8: Analytical WPs March March 2008 Mx.1First project meeting, discussion of methods and sample strategy, Dx.1 Approach strategy for sample selection March March 2009 Dx.2. Final plan with adjustment based on field results WP6: M Avenin patterns from 1st multiplication by P5

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP9: Analysis for cold and drought tolerance Question: starting not before month 24?

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Milestones and Deliverables in the WPs (2007/2008) WP10: Project documentation and internet portal March March 2008 M10.1, D10.1Accessions for the working collection selected M10.2, D10.1Project web portal launched M10.3Module for online input of germination and harvest data developed M10.4Module for online randomisation of field plans developed March March 2009 M10.5, D10.3Online interface for creating templates for field observation developed M10.6Refined object model for C+E data developed M10.7Online upload of C+E data developed

Avena genetic resources for quality in human consumption (AVEQ) Startup Meeting, Clermont Ferrand 2007 Thanks for your attention