Effects on lipid levels and lipid metabolism under more human-like conditions. –Lipid levels, cholesterol synthesis and cholesterol absorption. Effects.

Slides:



Advertisements
Similar presentations
Long-term Complications of Type 2 Diabetes
Advertisements

Pathogenesis of Acute Coronary Syndromes Nathan Wong
Difference in lipid metabolism and atherosclerosis among human, rodents, and WHHL / WHHLMI rabbits.
Lukasova M. et al. J Clin Invest 2011;121:
The Healthy Heart Figure 14.1.
Effect of garlic on inflammation processes and atherosclerosis under human-like conditions in APOE*3-Leiden mice Sonia M.S. Espirito Santo TNO Prevention.
Lipids: Not Just Fat BIOL 103, Chapter 6-2.
Mosby items and derived items © 2006 by Mosby, Inc. Slide 1 Chapter 19 Coronary Heart Disease and Hypertension.
بسم الله الرحمن الرحيم.
Cholesterol and C.V.D.. Learning outcome Most cholesterol is synthesised by the liver from saturated fats in the diet. Cholesterol is a component of cell.
By- Katherine Kauffman Vanessa Witmer. Brief Steroid Information Steroids are the third major class of lipids. Their structure is basically four rings.
Lipids (Lipid Profile)
Role of PXR Signaling in Mediating the Cardioprotective Effects of  -3 Fatty Acids Saraswathi Viswanathan, Ph.D. Assistant Professor Department of Internal.
RISK FACTORS FOR ATHEROSCLEROSIS IN OBESE CHILDREN  Dyslipidemia High triglycerides, VLDL Low HDL High small, dense LDL  Glucose intolerance, diabetes.
Lipoproteins Metabolism
Lipoproteins Clusters of lipids associated with proteins that serve as transport vehicles for lipids in the lymph and blood.
 The third major class of lipids  The three cyclohexane rings (A, B, C) are connected to a cyclopentane ring (D).  Significantly different structure.
Chapter 33.2: Blood and Heart Disease
BCH 348: Metabolism-II Dr Nikhat Siddiqi.
1 Human Intervention Study From Report of the EU reviewers (Midterm review , Brussels) “The human studies need careful thought otherwise there.
Copyright® Raisio The effect of a very high daily plant stanol ester intake on serum lipids, carotenoids, and fat-soluble vitamins Gylling et.
Stacy Gaugler and Lindsey Heiser April 28,2011.  The third major class of lipids is the steroids.  They are compounds containing: ◦ 17 carbon atoms.
Copyright® Raisio A plant stanol yogurt drink alone or combined with a low-dose statin lowers serum triacylglycerol and non-HDL cholesterol.
1 European Union Garlic Inflammation Study (EUGIS) History Garlic would influence lipid metabolism / atherosclerosis Proposed study: - 2 x 24 apparently.
13.9 Cholesterol.
Effect of garlic on inflammation processes and atherosclerosis under human-like conditions in APOE*3-Leiden mice Sonia M.S. Espirito Santo TNO Prevention.
Comparative Progression of Atherosclerosis in ApoE-/- Mice Exposed to ETS and CAPs Lung Chi Chen, Ph. D. Department of Environmental Medicine NYU School.
Main Topics of Research in the Health Part Effects of garlic powders and known compounds on cellular functions related to arteriosclerosis or inflammation.
Sonia M.S. Espirito Santo, Bart J.M. van Vlijmen, Rien Buytenhek, Wim van Duyvenvoorde, Erik H. Offerman, Louis M. Havekes and Hans M.G. Princen Gaubius.
Third major class of lipids. It is a compound that contains three cyclohexane rings A. Cholesterol The most abundant steroid in the human body. The most.
Copyright® Raisio Effects of long-term plant sterol or stanol ester consumption on lipid and lipoprotein metabolism in subjects on statin treatment.
Lipoproteins The serum lipoproteins are complexes of lipids and specific proteins called "apoproteins". Functions of Lipoproteins Help to transport lipids.
Lipid metabolism Chapter 26 Chem 454: Biochemistry II University of Wisconsin-Eau Claire Chapter 26 Chem 454: Biochemistry II University of Wisconsin-Eau.
بسم الله الرحمن الرحيم.
Dietary phosphate restriction induces hepatic lipid accumulation through dysregulation of cholesterol metabolism in mice Nutrition Research Journal May.
بسم الله الرحمن الرحيم.
Rolf Gebhardt University of Leipzig Medical Fakulty Institute of Biochemistry EU-Project: Garlic & Health Effects of High Quality Garlic on Markers of.
Kristin Miller Caleb Conrad 13.9 What Are Steroids.
Effect of garlic on inflammation processes and atherosclerosis under human-like conditions in APOE*3-Leiden mice Sonia M.S. Espirito Santo 1,2, Wim van.
Clinical diagnostic biochemistry - 8
Hyperlipidemia Hyperlipidemia and hyperlipoproteinemia are general terms for elevated concentrations of lipids and lipoproteins in the blood. hypercholesterolemia.
Atherosclerotic lesion area within the aortic sinus of apoE - / - and apoE - / - - hCRP + mice Gideon M. Hirschfield et al. PNAS 2005;102:8309– 8314.
Plant stanol and sterol esters - mechanism and safety issues -
HANDBOOK OF DYSLIPIDEMIA AND ATHEROSCLEROSIS Part One Professor Jean-Charles Fruchart Department of Atherosclerosis (Inserm UR545) Pasteur Institute of.
 Define cholesterol.  Discuss the purpose of lipoproteins.  Distinguish between LDL and HDL.
Vitamin C, Lipoprotein(a), and Atherosclerosis
Atherosclerosis. Atheroma are not merely filled with lipid, but contain cells whose functions critically influence atherogenesis: Intrinsic Vascular Wall.
LIPIDS Study Guide Pg 171.
Lipoproteins and Atheroscloresis
Lipoproteins and Atheroscloresis
بسم الله الرحمن الرحيم.
Apolipoproteins, Lipid Transfer Proteins, and Lipoprotein Metabolism
Note World Health Organisation indicated on 5 March
Increased atherosclerotic lesion area in apoE deficient mice overexpressing bovine growth hormone  Irene J. Andersson, Anna Ljungberg, Lennart Svensson,
Intestinal Villi.
LIPIDS AND LIPOPROTEINS Dr. Gamal Gabr
Genes Within the MHC Region Have a Dramatic Influence on Radiation-Enhanced Atherosclerosis in MiceClinical Perspective by Weibin Shi, Zhimin Zhang, Mei-Hua.
Arterioscler Thromb Vasc Biol
Serum Triglyceride Test
Volume 15, Issue 1, Pages (January 2012)
Hepatic low-density lipoprotein receptor–related protein deficiency in mice increases atherosclerosis independent of plasma cholesterol by Sonia M. S.
Type 2 diabetes: Overlap of clinical conditions
Advanced Nutrition Diet and Cardiovascular Disease 1
بسم الله الرحمن الرحيم.
Volume 12, Issue 2, Pages (August 2010)
Volume 13, Issue 8, Pages (November 2015)
Hajer GR et al. Atherosclerosis 2009;202:216-24
Lipoprotein Metabolism
Fig. 2. CD treatment facilitates regression of murine atherosclerosis.
Effects of pterosin A on serum levels of lipid, blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST), and alanine aminotransferase (ALT)
Presentation transcript:

Effects on lipid levels and lipid metabolism under more human-like conditions. –Lipid levels, cholesterol synthesis and cholesterol absorption. Effects on fast atherosclerosis development and other processes that might be related with atherosclerosis. –Inflammation, blood pressure, platelet activation and atherosclerosis analysis. Aim of the study: Effects of garlic on lipid metabolism and atherosclerosis

APOE*3-Leiden transgenic mouse as a model for hyperlipidemia and atherosclerosis studies Transgenic mice carrying a mutant form of human APOE*3 gene. Dominant dysfunctional APOE*3-Leiden results in defect in chylomicron/ VLDL clearance and more human-like lipoprotein profile. Highly responsive to feeding cholesterol-rich diets. Develops atherosclerosis varying from fatty streaks to more human-like complicated lesions.

Effect of garlic on lipid metabolism and atherosclerosis in APOE*3-Leiden mice: Selection of compounds Lichtwer LI111 garlic powder: Reference dry garlic powder. Morado de Cuenca 200 (Mor200): Garlic powder rich in organic sulfur-containing compounds. Diallyl disulfide (DADS): Isolated garlic compound rich in sulfur. Allicin: Reference isolated garlic compound reported to result in 43% reduction of fatty streaks in C57Black/6 mice (D. Abramovitz et al., Coronary Artery Disease, 1999, 10: )

Effect of garlic on lipid metabolism and atherosclerosis in APOE*3-Leiden mice: Effect of garlic on lipid metabolism and atherosclerosis in APOE*3-Leiden mice: Experimental scheme -2 Matching and start of the study Weight and food intake Blood sampling/ plasma isolation Plasma lipid levels Cholesterol lipoprotein profile Chol. synthesis/ Intestinal absorption Platelet activation measurements Marker for liver function Liver lipid composition Atherosclerotic aorta lesions area Inflammation markers p p 60 p 80 p p 65 p p p 8065 p pp p Regular dietWestern dietAtherogenic diet 60 p Blood pressure Compound biostability 65 pp 80 p 5 65 p 15 Run in p p 0 Atheroscleroticaorta lesions typing60 5 General parameters Inflammation and atherosclerosis related parameters Lipid metabolism related parameters 80

Effect of garlic on plasma cholesterol levels under 3 dietary conditions *P< 0.05 Plasma cholesterol levels Regular dietWestern dietAtherogenic diet mM Control LI Mor * DADS Allicin 3.5 * 9.273

Effect of garlic on markers of cholesterol synthesis and intestinal absorption under 3 dietary conditions Regular diet *P< 0.05 Markers of cholesterol synthesis Markers of intestinal absorption

Effect of garlic on liver function under 3 dietary conditions t P=0.051 Regular dietWestern dietAtherogenic diet Control LI Mor DADS Allicin Plasma alanine amino transferase t t U/L

Effect of garlic on Serum Amyloid A and von Willebrand factor concentrations under fast atherogenic conditions SAA (µg/ ml) Control LI111Mor 200DADS Allicin vWF (%PP) Control LI111Mor 200DADSAllicin * * p=0.001 p<0.001 *P< 0.05

Control Primador FRANCE 2001: 0.64 g/kg b.w. of dry garlic powder with low sulphur content. Primador FRANCE 2001: 6.4 g/kg b.w. of dry garlic powder with high sulphur content. Kyolic (positive control): 200 mg/kg b.w. of powder rich in S- allylcysteine reported to result in 64% reduction of fatty streaks in New Zealand white rabbits. Campbell JH, Efendy JL, et al. J.Nutrition. 2001;131:1006S-1009S. Effect of garlic powders on atherosclerosis under more human-like conditions: Groups selection

Effect of garlic powders on atherosclerosis under more human-like conditions: Experimental design Matching and start of the study Weight and food intake Blood sampling/ plasma isolation Plasma lipid levels Cholesterol lipoprotein profile vWf, t-PA and soluble ICAM Marker for liver function Liver lipid composition (p.m.) Aortic root lesions composition Aortic root lesions typing and area p 55 Diet T + 0.2% cholesterol 55 p SAA and Fibrinogen Compound biostability 60 p 5 Run in Aortic arch area and lipids deposition55 5 General parameters Inflammation and atherosclerosis development related parameters Lipid metabolism related parameters In weeks p 55 All blood stimulation assay 55 p pppppp p p Mice on standard chow diet8