NCBI FieldGuide NCBI Molecular Biology Resources January 2008 Using Entrez.

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Presentation transcript:

NCBI FieldGuide NCBI Molecular Biology Resources January 2008 Using Entrez

NCBI FieldGuide WWW Access Entrez & BLAST

NCBI FieldGuide Gene Homologene Entrez: Database Integration PubMed abstracts Nucleotide sequences Protein sequences 3-D Structure Word weight VAST BLAST Hard Link Neighbors Related Sequences Neighbors Related Sequences BLink Domains Neighbors Related Structures

NCBI FieldGuide The Links Menu: Access to Neighbors and Links SNP GEO Gene PubMed Protein

NCBI FieldGuide The Links Menu: Access to Neighbors and Links Neighbors: BLAST Link pre-computed BLAST Neighbors: BLAST Link pre-computed BLAST Neighbors: pre-computed CDD search Neighbors: pre-computed CDD search

NCBI FieldGuide The Links Menu: Access to Neighbors and Links Neighbors Hard Links

NCBI FieldGuide Database Searching with Entrez uUsing limits and field restriction to find human MutL homolog uLinking and neighboring with MutL uMapping SNPs onto structure

NCBI FieldGuide Global NCBI (Entrez) Search colon cancer

NCBI FieldGuide Global Entrez Search Results

NCBI FieldGuide OMIM: Human Disease Genes Conserved Domain

NCBI FieldGuide Nucleotide Sequences Nucleotide database now three parts EST expressed sequence tags GSS genome survey sequences CoreNucleotide everything else

NCBI FieldGuide Core Nucleotide Results with Gene Preview New! Gene Preview more relevant results New! Gene Preview more relevant results New! Taxonomy Filters

NCBI FieldGuide Advanced Search Options Tabs Taxonomy filter

NCBI FieldGuide More Precise Nucleotides Search colon cancer[Title] AND nonpolyposis[Title] AND human[Organism] AND biomol_mrna[Properties] AND srcdb_refseq[Properties]

NCBI FieldGuide Useful Field Restrictions [Title]: Definition line in GenBank / GenPept format shown in Summary format glyceraldehyde 3 phosphate dehydrogenase[Title] [Organism]: NCBI’s taxonomy. Organizing system for molecular databases mouse[organism]; green plants[organism]; Streptomyces coelicolor[organism] [Properties]: molecule type, location, database source biomol_mrna[properties]; biomol_genomic[properties]; gene_in_mitochondrion[properties]; srcdb pdb[properties] [Filter]: subsets of data, Entrez links all[filter]; nucleotide mapview[filter]; nucleotide omim[filter]

NCBI FieldGuide Entrez Tip: Start Searches in Gene UniGen e Other Entrez DBs BLink Homologene: Gene Neighbors

NCBI FieldGuide Gene Results nonpolyposis colon cancer AND human[Organism]

NCBI FieldGuide Precise Results MLH1[Gene Name] AND Human[Organism] NCBI Taxonomy

NCBI FieldGuide Organism Field: NCBI’s Taxonomy All molecular databases All molecular databases

NCBI FieldGuide MLH1 Gene Record

NCBI FieldGuide MLH1 Gene Record: Interactions and GO

NCBI FieldGuide MLH1 Sequences

NCBI FieldGuide MLH1 Gene Record: Sequences

NCBI FieldGuide MLH1: Sequence Links

NCBI FieldGuide Gene Table: Genomic Sequences

NCBI FieldGuide Map Viewer: All Sequences Customizable NCBI Assembly EST Hits Gene Annotations Models Transcripts Download data and sequences

NCBI FieldGuide MLH1 Homologs

NCBI FieldGuide Synteny: Mammalian Genomes Albumin Gene Family

NCBI FieldGuide Finding Homologs: HomoloGene Gene

HomoloGene Cluster Gene Links Protein Links

HomoloGene Downloader Protien mRNA Genomic Protien mRNA Genomic

NCBI FieldGuide Finding Homologs 2: BLink Gene Protein

NCBI FieldGuide BLink: BLAST Link (Best Hits) Redundant Proteins First 200 only BLAST Tomato homolog

NCBI FieldGuide Finding Polymorphisms Gene

NCBI FieldGuide GeneView: Variations Human MLH1 ATPase domain

NCBI FieldGuide MLH1 Structure Model and Mapping Polymorphisms

NCBI FieldGuide Related Structures: structure model

NCBI FieldGuide Sequence Similar Structures Conserved Domain Conserved Domain Link to Structure Link to Alignment

NCBI FieldGuide E. coli MutL Structure Cn3D viewer Conserved Domains

NCBI FieldGuide Alignment Based Model : Mapping Polymorphisms Mg 2+ binding site Ile - Val

NCBI FieldGuide Better Model: Conserved Domain Gene Protein Related Structures

NCBI FieldGuide Better Model: Conserved Domain Mg 2+ binding site Ile – Val Position 32 Ile – Val Position 32