Previously in Cell Bio Hypotheses for ‘problem’ in Graves’ Disease Positive signals (TRH or TSH) altered to increase amount or affinity for their receptor Signal receptor altered to increase their their affinity for hormone Problem with signal relay ‘inside’ thyroid Signals detected via binding interactions Binding interactions governed by protein folding Protein folding dictated by amino acid sequence

What do we know so far? Thyroid is ‘overacting’ Pituitary normally responsible for thyroid stimulation through levels of TSH Graves’ patients have normal/decreased levels of TSH in blood Binding affinity between TSH and TSH-R normal

More of what we know TSH is water soluble hormone Figure 4-1. Schematic drawing of human TSH, based on a molecular homology model built on the template of a hCG model14. The a-subunit is shown as checkered, and the b-subunit as a solid line. The two hairpin loops in each subunit are marked L1, L3; each subunit has also a long loop (L2), which extends from the opposite site of the central cystine knot. The functionally important a-subunit domains are boxed. Important domains of the b-subunit are marked directly within the line drawing (crossed line, beaded line and dashed line): For further details the reader is referred to Grossman et al.2. (Reproduced from Grossman,M, Weintraub BD, SzkudlinskiMW- Endocrin Rev (4) 18: ,1997, with permission of the Endocrine Society). From “The Thyroid manager”

Even more Thyroid plasma membrane is barrier to polar molecules TSH interacts with a receptor on the surface of thyroid cells HOW and WHY is the thyroid responding as though over-stimulated? How do signals get passed across membranes?

Characteristics of Transmembrane Proteins Hydrophobic face of protein in transmembrane region -one continuous structure or multiple regions of 2° structure Charges ‘anchor’ transmembrane region Asymmetric orientation

Peripheral Membrane proteins Characteristics Association with membrane not as strong Various means of attachment -Protein-protein -Protein-phospholipid head Fig 3-32 Molecular Cell Biology by Lodish et al.

Membranes and membrane proteins How can a polar signal gain access to the cytosol Direct access: From the ‘outside’ Pores Channels Pumps From cytosol to cytosol Gap junctions

Membrane proteins Indirect access: Receptors TSH Receptor: from “The Thyroid Manager” Ch16 Extracellular domain Cytoplasmic Domain Plasma Membrane If signaling molecule never gains access to cytosol how can the information be transmitted?

Transmembrane receptors Same general structure as other transmembrane proteins Able to bind specific ligand Ligand binding causes conformational change What change in the TSH receptor could cause overproduction of T3 and T4 How could you test your hypothesis?

Allosteric transitions What are they, why are they important, How do they relate to signal transduction R T state transitions Cooperative binding Check out CBI 3.9 Chime model to watch an example of both

Other mechanisms that regulate protein function Compartmentalization Change in rate of synthesis Common traits? Cleavage Phosphorylation/dephosphorylation Common traits?

Receptor’s role (summary) Able to transduce signal because of: Placement in membrane (span it) Ability to bind ligand Ligand -induced conformational changes So the signal ‘gets in’ without physically crossing membrane How do you go from a shape change to causing a change in gene expression?

2nd Messengers and Signaling Cascades Getting the signal to where it needs to go