Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg.

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Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg June 30, 2008

Two F 2 populations produced DNA extracted - Qiagen DNeasy 96 plant kit AFLP fingerprinting technique Genomic DNA digested - PstI and MseI BSA performed PstI+MseI primer pairs PCR ABI Genetic Analyzer A genetic map produced - JoinMap Materials and Methods

M RP SP Primers: P-acg+M-cga Results F 2 population from CN56979/CN

Results - Mapping PaatMccg150 PaaaMcga2852 PaaaMcga5 PaggMcaa1558 PaggMcaa20515 PaggMcaa19016 PaccMcgg23022 PacgMcga37028 Sr_ PaagMctc14062 PaagMctc13063 PaaaMcga33064 PaagMctc11066 PacgMcga12071