The Ecological Engineering Group Biological and Agricultural Engineering University of Arkansas Algal Growth with Nutrient Limitation Compared to Light-Limiting.

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Presentation transcript:

The Ecological Engineering Group Biological and Agricultural Engineering University of Arkansas Algal Growth with Nutrient Limitation Compared to Light-Limiting Conditions in Ozark Streams Andrea Ludwig Marty Matlock, PhD, PE, CSE Brian Haggard, PhD Bob Morgan, PE

EPA use attainability study

Research Goals Identify a nutrient, periphytic chlorophyll-a relationship in Ozark streams Investigate circumstances of light- limitation to native algae Predict response of algal growth to differentiating canopy covers in the presence of variable nutrients

Methodology Matlock periphytometers deployed at all sites Chlorophyll-a used as indicator of abundance of algae Chlorophyll-a analysis done using trichromatic methods Grab samples collected at each site and analyzed for nutrient concentrations Light meters attached to Matlock periphytometers at selected sites Site Selection Coupled sites according to locations of WWTPs

Site Selection

The Matlock Periphytometer Assembly NutrientSolution Lid w/ hole Glass fiber filter 0.45  m Nylon Membrane 1 liter bottle Nutrient Solution Nutrients passively diffuse through nylon membrane and GFF Flow

The Matlock Periphytometer In the field

Target PO 4 -P Level Annual - average PO 4 -P (ppb) Annual - average chl a (ug/L)

Methodology 2-day and 14-day deployments of light meters Sites chosen with comparison of light availability as single variable Canopy cover used as indicator

Methodology Hardware HOBO Micro Station Sensed temperature and photosynthetic active radiation (PAR) Reading every 5 sec Logged every 10 min Software BoxcarPro

Results Phosphorous concentrations only account for 7% of variability of chlorophyll-a across sites

Results Site Avg Temp C Avg PAR uE Chlr a ug/cm2 OSG SPG931UP SPG931UP SPG931DN OSG930UP OSG930DN OSG045UP OSG045DN Two-day deployments of light meters provided inaccurate representation of average light availability to periphytometer

Results 2-day deployments failed to give good representation of light conditions at sites 14-day deployments logged definite diurnal cycles and more data points

Due to time restrictions, only one round of 14-day deployments were completed Results

Summary Nutrient concentrations do affect algal growth However, when nutrients are high and light is low, algal growth becomes light limited at very low production levels Riparian zone management is critical for protecting nutrient enriched streams

Future Work / Improvements Additional study of nutrient-limited situations in Ozark streams Removing any variability of nutrients in sites Deployment of light meters and Matlock periphytometers at same site with different canopy covers Defining a relationship of chlorophyll-a and nutrient concentrations with a second variable of light Multiple light meter couples at sites with multiple nutrient and canopy characteristics

Acknowledgements Eric Cummings Brian Schaffer Matt Bruton Debrata Sahoo Sujit Ekka Kyle Kruger