Staining of Bacteria Staining of Bacteria Brno University of Technology, Faculty of Chemistry Hana ŠURANSKÁ

Staining A biochemical technique of adding a class-specific dye Stains and dye – to highlight cellular structures DNA, proteins, lipids, carbohydrates Different stains react in different parts of a cell or tissue Microscopes

Dye Dye A colored substance Used to highlight cellular structures Applied in aqueous solutions Carmine, crystal violet, DAPI, eosin, ethidium bromide..

Eosin A red fluorescent dye Stains cytoplasm, collagen and muscle fibers Most often used as a counterstain An acidic dye It shows up in the basic parts of the cell (nucleus)

DAPI DAPI 4,6-diamidino-2-phenylindole A fluorescent stain It binds strongly to DNA and RNA Used in fluorescence microscopy Excited with ultraviolet light

Methods of staining Gram staining Gram staining Gimenez staining Gimenez staining Giemsa staining Giemsa staining Ziehl-Neelsen staining Ziehl-Neelsen staining Eosin staining

Gram staining Gram staining Hans Christian Gram (1853–1938) An empirical method of differentiating bacterial Gram-positive, Gram-negative in 1884 in 1884 It uses crystal violet, iodine, fuchsine, safranin

Giemsa staining Gustav Giemsa It can be used to study the adherence of pathogenic bacteria to human cells A mixture of methylene blue and eosin Visualize chromosomes

Ziehl-Neelsen staining Ziehl-Neelsen staining The acid-fast stain Franz Ziehl and Friedrich Neelsen A special bacteriological stain It is used to identify acid-fast Mycobacteria Micture of carbolfuchsine and methylene blue Mycobacterium tuberculosis

Thank you for your attention!