Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results.

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Presentation transcript:

Discover the UniProt Blast tool

Murcia, February, 2011Protein Sequence Databases Customize the BLAST results

Look for the local alignment

Highlight important positions within the alignment according to the sequence annotation The metal binding sites are conserved

HBB_HUMAN

Discover the UniProt Align tool

1- Select sequences of interest… (from Blast results for example ) 2 - Align…

Add the ‘query’ sequence (HBB_HUMAN)

Conservation of the metal-binding site…. Highlight important positions within the alignment according to the sequence annotation

Compare the UniProt NCBI

Protein Sequence Databases The ‘alternative’ sequence(s) not ‘directly available’ for a lot of tools, including protein identification tools, Blast, depending on the server !…. Murcia, February, 2011

Blast P04150 against Swiss-Prot / homo UniProt Isoform sequences Murcia, February, 2011Protein Sequence Databases

Blast P04150 against Swiss-Prot / homo NCBI The isoform sequences (from Swiss-Prot) are not present in the NCBI protein database ! The.x number (P ) correspond to the version number of the sequence…not to an alternatively spliced sequence ! Murcia, February, 2011