Varian 920-LC Integrated Analytical HPLC

Varian 920-LC What Is It? Compact, stylish automated analytical HPLC Pre-plumbed and factory tested for the highest confidence Single cable communications Each system comes with solvent delivery system, autosampler and either UV Vis or PDA detector Options of a second Fl or RI detector and column heater Virtually pulse-free dual piston pump Does not require a pulse damper State-of-the-art low pressure quaternary gradient module with built-in degasser

Varian 920-LC What Is It? Autosampler with cooling option Synchronised injection for outstanding retention time reproducibility Compatible with 200 x 2 mL HPLC vials or 3 microplates Either sensitive dual l UV-Vis or PDA detector Optional second detector - fluorescence or RI High precision (<0.15 °C) dual column heater available Low delay solvent pre-heater for improved heat transfer Optional column switching and electric injection valve NEW improved instrument interface on GalaxieTM software

Varian 920-LC Silver Bullets Fast LC with superb performance optimised for sub 3 mm columns Improved throughput without compromising robustness Pulse-free solvent delivery No need for a pulse damper High sensitivity detection Full system level computer controlled AIQ (validation)

Varian 920-LC Fast LC Phenones 0.1 0.2 0.3 0.4 0.6 0.5 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 mAU RT [min] 100 90 80 70 60 110 40 30 50 20 10 Conditions Mobile Phase: Water Acetonitrile Gradient Detection: UV Detection Temperature: 60°C Flow-Rate: 1,6mL/min Column: Varian Ultra 3um 50x2,0mm acetophenone Propiophenone valerophenone benzophenone hexanophenone heptophenone octanophenone

Varian 920-LC Fast LC PAHs

Varian 920-LC Fast LC Performance Dual piston quaternary pump produces fast gradients – even at 200 mL/min Virtually pulse-free pumping without pulse damper Outstanding gradient compositional accuracy give retention time precision at the <1% level Built-in vacuum degas maintains pump prime and enhanced injection reliability Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns Overlay of 10 chromatograms 10% steps at 1 mL/min

Varian 920-LC Autosampler Needle-in-flow path geometry for low carry-over (<0.01%) Typical inj precision (< 0.3% at 10 mL) Optimized flow paths for fast injection turn-around Synchronised injection for best RT reproducibility Programmable wash setting for low carry-over Injection volumes from 1 uL to 50 mL Cooled option available

Varian 920-LC Superb Washout 125 µg/mL Caffeine Blank injection <0.01% carryover

920-LC Autosampler How Does it Work?

Varian 920-LC Injection Synchronization

Sensitive UV-Vis Detectors Typical Agilent VWD S/N ~ 25 Typical Varian 920-LC UV Vis S/N ~ 38

Varian 920-LC Fluorescence Detector Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method) Time programmable l for increase sensitivity Automatic l validation with built-in Hg lamp Low 12 mL dead volume flowcell for improved chromatography

Heated Column Compartment Temperature range from ambient + 10 °C up to 65 °C Temperature reproducibility +0.15 °C Ensures excellent analytical reproducibility Uses a low delay volume pre-heater for efficient heat transfer to mobile phase Spacious column compartment easily accommodates 2 x 250 mm columns Option with electrically actuated column switching valve and injection valve

Outstanding Stability

System Reproducibility of 50 Injections Reproducibility <0.8% over 50 injections and 12 hours

Varian 920-LC Software Usability User friendly GalaxieTM software Simplified, modern, consistent Instrument interface Modular 11 pages 920-LC 4 pages!! One page real-time status and chromatogram Advanced integration and calibration New IMS capabilities Automated instrument validation Reduces downtime and operating costs Fusion AE

Varian 920-LC AIQ (Validation) AIQ (Analytical Instrument Qualification) Fully PC controlled IQ and OQ (not PQ) Puts the Varian 920-LC at the forefront of regulated capabilities Consistent, reproducible methodology Includes pumps, autosampler, detectors, column heater Chargeable option Fully documented

Varian 920-LC Ordering Matrix pump inject detector Oven Detector 2 option Options Modular Isocratic 210-LC 10mL Manual 325 PDA, RI, ELSD Isocratic 00-100890-00 Dual piston pump 10 mL head Auto Dual l UV/Vis Opt FL, RI ELSD Gradient 00-100891-00 Quat, Grad, Degas ELSD opt High Performance Gradient 00-100892-00 Auto cooled Development 00-100893-00 PDA Std Research 00-100894-00

Varian 920-LC Target Markets Strong historical position for Varian in analytical Varian has the leading range of analytical technologies: spectroscopy, chromatography including consumables, data and services Target Users Environmental Industrial Generic Pharmaceutical * Academia and Biotech Same (but wider) target groups with a stronger product offering *40% of Regulated Pharma has significant competitive barriers due to installed software base

Varian 920-LC Silver Bullets Fast LC with superb performance optimised for sub 3 µm columns Improved throughput without compromising robustness Pulse-free solvent delivery No need for a pulse damper High sensitivity detection Full system level computer controlled AIQ (validation)

Varian 920-LC Integrated Analytical HPLC