Metabolism Summary.

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Presentation transcript:

Metabolism Summary

Energy Define Types High Energy Compounds Formation Other definitions Examples Use Formation Processes Byproducts Other definitions Calorie Exergonic Endergonic

Metabolism Define Parts/Divisions Requirements E pathways Proteins Fats Carbohydrates

Enzyme Define Parts Examples and function Denature Inhibitors Types Competitive Noncompetitive Reactions Reversible Irreversible

Enzyme Inhibition Noncompetitive

Carbohydrate Catabolism Types Embden-Meyerhof (EM) Pentose Phosphate Pathway (PPP) Entner-Duodoroff (ED) Compare / Contrast Location Starting substrate Important steps “End” products (intermediaries)

EM

PPP

ED

Anaerobic Pathways Pathway EM PPP ED Location Substrate Cytoplasm Glucose G6P Steps 2 parts 6C  3C Different sugars (4-7C) ribulose Phosphogluconic Glyceraldehyde Products 2 Pyruvate 2 ATP 2 NADH 2 H+ Pyruvate & Fructose 6 CO2 12 NADPH 12 H+ 1 ATP 1 NADPH 1 NADH

Glycolytic Pathways used by various Bacteria Bacterium E-M PPP E-D Acetobacter aceti - + Bacillus subtilis major minor E. coli Lactobacillus acidophilus Pseudomonas aeruginosa Vibrio cholera

Fermentation Define Process Names Start End By-products Acid Alcohol ATP

Aerobic Respiration Define Where occurs Process Pathways Requirements TCA OP Requirements Substrate Products Importance

Pyruvate pathways

TCA

Redox

ETC Redox reactions

Eukaryotic ETC

Prokaryote ETC location

Animation

Prokaryotic ETC ATP formation

Anaerobic Respiration Use of another compound than O2 as final electron acceptor in the ETC Examples Nitrate ion NO3- [Pseudomonas, E coli, Bacillus] NO2- N2O N2 Sulfate ion SO4= to H2S Methanogens Carbonate ion CO3= to CH4

Metabolic Pathway Comparison animation

Micro Laboratory Tests Acid Fermentation CO2 production Oxidase Nitrate reduction Methyl Red Voges-Proskauer TSI Decarboxylation TSI Deamination Enzyme digestion (starch, lipid, protein) VP +

Biosynthesis

Functions Polysaccharide Cell wall components LPS Lipid Cell wall Plasma membranes AA/Protein Cell wall / membrane components Enzymes Toxins Nucleotide DNA RNA ATP NAD NADP

Metabolism Summary

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