LDC-CEVA Cevac IBird April 2015

CONTENT Presentation of LDC group French Infectious Bronchitis epidemiology Actions Monitoring implementation Results

LDC - Overview From its creation in 1970, LDC is today… 62 production sites in France and 5 platforms 5 100 commited farmers More than 16 000 employes, 90% in France Activities : 85% in poultry, 15% in convenience 3 billions € in turnover from which 15% in subsidiaries abroad (Poland and Spain) 100 Millions Euros of investments in 2013 from which 95 % in France A Group present in every poultry region in France Brittany 10 sites 1 700 employees 500 farmers Pays de Loire / Centre / Normandy 35 sites 9 600 employees 3 500 farmers Aquitaine 2 sites 250 employees 100 fermers South-East 13 sites 2600 employees 1000 farmers Paris Production sites in poultry production areas : Brittany, Loire Valley, Normandy, Burgundy, Aquitaine,… LDC Group is the french leader of poultry production. The group’s companies are mainly located in France. There is also an international division with companies in Poland and Spain. 3

Slaughterhouses France International Division LDC - The Group Organization Convenience Division Poultry Division International (group Export) Upstream France Slaughterhouses France International Division POLAND SPAIN At the beginning in 1970 only 1 slaughterhouses Expansion of the company by mainnly External growth Uptream division joined the group in 2001. Now 3 division.

Direction : Gilles HUTTEPAIN Upstream Division LDC - Group Organization Eggs Grain Collect Poultry Poultry 15 companies -Poultry production in West area (Brittany, Loire valley,..) and East area (Burgondy). -Feed production -Eggs production Direction : Gilles HUTTEPAIN

LDC – Group organization – The Products In front of the Group needs Upstream Division Number of farmers 1 440 Surface 1 970 000 m² Turnover 610 M€ Production of poultry In front of the Group needs 70 % Chicken 15 % Label rouge chicken 60 % Turkey 50 % Duck 40 % Guinea fowl Eggs/boxes/year 325 millions eggs Label Rouge, Free range, & Organic 150 millions eggs Standards 475 millions eggs From which 50% under brands Loué & Le Gaulois Tons Feed /year Poultry 780 000 T From which processed 700 000 T Collect Cereals/year 178 000 T 1440 Farmers 1970000 m2 uptream division is producing only a part of the group live poultry need. 6

Poultry Division LDC - The Group Organization LDC Sablé+ CEPA Cavol Slaughterhouses near poultry production (30) Direction : Denis LAMBERT

Poultry division France LDC - The group Organization – The Products Poultry division France Mix customers Retail / HD 67 % Prof & Indust 25 % EXPORT 8 % Turnover : 2,1 Bn € Volume : 460 000 T Mix Product Poultry /week 2 500 000 Standard chickens 150 000 Guinea fowls 1 000 000 Label chickens + Org 104 000 Rabbits 340 000 Certified chickens 300 000 Quails 390 000 Turkeys 40 000 Squabs 315 000 Ducks Further-processed products 1 500 T per week Mix product per week Costumers

Poultry and convenience : synergy and complementarity LDC - Our Strategy : Innovations and Brands for a strong organic growth Poultry and convenience : synergy and complementarity in offers Convenience Further processed Cut ups Red Label Poultry Poultry Here is Product range. Whole poultry mainly Red label, Cuts, cooked poultry meat ans cooked meals. The product are marketed in our own brands like LE GAULOIS and MAITRE COQ or MARIE.

French Infectious bronchitis epidemiology % of broiler production per department 830 million broilers 43 million layers 45 millions turkeys 120 million ducks ….Multi species production Infectious bronchitis viruses are circulating. Need for a good viral protection

French Infectious bronchitis epidemiology Vaccination with Mass vaccine is done on 100 % of the birds, at the hatcheries. Field boosting with either 793 B or QX are applied according to veterinarian, integration or farmer decision related to IB perceived pressure or type of broilers produced (big birds). D0 D10-D14 D16-D20 IB Mass Strain (Hatchery) IB Variant Strain (Field vaccination) 793B/QX GUMBORO We used to have this vaccination program before ibird.

French Infectious bronchitis epidemiology => Vaccination at the hatchery is not perceived as critical by the independant hatcheries => Field vaccination is usually applied by spray with no regular monitoring. Need for LDC -to get better control of vaccine & protection on the production. -to get better evaluation of epidemiological situation in real time 2 concerns with that program 12

Actions : a new vaccination program Big birds Certified birds D0 D16-D20 HATCHERY IB Vaccination. Monitoring Implementation IB Mass Strain + IB Variant strain (Cevac IBird). Hatchery GUMBORO Hatchery vaccination with Mass & 793 B vaccine (Cevac IBird) has been decided for big birds or certified birds slaughtered in 3 main LDC slaughter houses. monitoring implementation.

Actions 3 different slaughterhouses 230 different poultry houses Vaccination Mass & Cevac IBird spray day 1 230 different poultry houses In 2 regions from nov 14. Control of spray equipment & CHICK Program implementation 7 different hatcheries Monitoring of day 10 PVR & respiratory signs during growing period 3 different organisations 3 different slaughterhouses

Monitoring implementation Vaccination Mass & Cevac IBird spray day 1 230 different poultry houses In 2 regions from nov 14. Control of spray equipment & CHICK Program implementation 7 different hatcheries Sampling & Analysis for epidemiological monitoring Monitoring of day 10 PVR & respiratory signs during growing period 5 different organisations 3 different slaughterhouses 10 Blood samples per flock : Biocheck Elisa Cecal tonsils: frozen and PCR analysis according to Elisa & farm results 15

Hatchery spray evaluation Desvac In-Line sprayer Desvac In-Line sprayer

Results : Safety 1 out of 230 flocks with mortality before 10 days, after veterinarian visit it was due to E coli infection 17

Results : IB ELISA MEAN TITERS

Field infection > 3000, low CV Results : IBV mapping October-December 2014, 28% of the farms are challenged by a field virus (T> 3000) No vaccination< 500 1000< « normal »< 3000 Doubtfull > 3000, high CV Field infection > 3000, low CV

Field infection > 3000, low CV Results : IBV mapping December 14 – February 15 , 38% of the farms are challenged by a field virus (T> 3000) No vaccination< 500 1000< « normal »< 3000 Doubtfull > 3000, high CV Field infection > 3000, low CV

Some respiratory signs after 10 days in 9 out of 230 flocks : Results : Some respiratory signs after 10 days in 9 out of 230 flocks : 1 IB titers OK 1 positive for ORT 1 positive for MS 2 IB PCR >0 no identification possible 4 IB PCR >0 for IBird, no conclusion other cause ? 21

Conclusion Hatchery vaccination with Mass & Cevac IBird gave consistent seroconversion at slaughter It can be used as hatchery vaccination monitoring Hatchery spray application was improved with equipement setting & control Field virus circulation was detected between 28 % and 38% of the farms according to the period. Reinforced the need of a complete and well applied vaccination program in our production area. 22