High Incidence of Lipid Deposition in the Liver of Rats Fed a Diet Supplemented with Branched- Chain Amino Acids under Vitamin B 6 Deficiency Tae Kaimoto, Mayumi Shibuya, Kazutaka Nishikawa and Hideo Maeda Department of Living Science Shikoku University, Tokushima, Tokushima Japan Elizabeth Katzman

What are Branched Chain Amino Acids (BCAA)?

How Does this Fit with B 6

Roles of BCAA  Suppresses fatigue in CNS.  Prevention of Hepatic Encephalopathy.  Recovery of burn victims.  Required for protein synthesis.  Contribute to energy metabolism.

Problems with Supplementation  Lack of information  High protein diets with B 6 deficiency  Biochemical parameters  Fatty liver

Previous Study Accumulation of triacylglycerol in the liver B6 Deficiency BCAAs 20% Casein

Hypothesis “It is possible that a disorder of amino acid metabolism in the presence of vitamin B 6 deficiency might influence the assembly of lipids and apolipoproteins and/or the secretion of lipoproteins.”

What are Apolipoproteins? studies-apolipoproteins

Diets and Animals  20% vitamin-free casein diet  21 days  Sacrificed

Isolation of Lipoproteins and Purification of Apo E Pooled serum of male Wistar Rats VLDL Preparative Ultracentrifugation APO E Column Chromatography

Purification of Apo E  Column Chromatography Chromatography/nav.htm?cid= &locale=en_US

Spectrophotometer

Serum and Hepatic Apolipoproteins  Enzyme-Linked Immunosorbent Assay (ELISA)

Enzyme-Linked Immunosorbent Assay (ELISA) linked+immunosorbent+assay

Lipoprotein Analysis  High Performance Liquid Chromatography (HPLC)

Growth Parameters

Serum and Hepatic Lipids

Serum and Hepatic Apolipoprotein Levels

HPLC Analysis of Serum

Conclusion “Therefore, it can be inferred from our data that the secretion of synthesized lipoproteins from the liver to the bloodstream might be impaired.”

What does it all mean?  Triacylglycerol and APO E are getting stuck in the liver  Fatty Liver Disease  Supplementation can do more harm than good.