Pre-Lab 19B: Acids, Bases and pH

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Presentation transcript:

Pre-Lab 19B: Acids, Bases and pH

Purpose Life exists inside a certain range of pH values. A pH value describes whether a solution is acidic, basic (alkaline), or neutral by describing the concentration of hydronium ions in a solution. An acid is a substance that produces hydronium (H3O+) ions when dissolved in water, and a base (or alkali) is a substance that produces hydroxide (OH-) ions when dissolved in water. Neutral solutions have equal numbers of H3O+ and OH- ions.

In this investigation, you will learn the pH of several common solutions by making a pH scale using a pH indicator and chemicals of known pH. You will also identify two ‘mystery’ chemicals (unknowns) with your created pH scale/table. You will be graded on lab performance and product accuracy/write-up

Key Question What is pH?

Background Define: pH Acid Base

Drawing Draw figure 19.27 from your text book

Section1: Make your pH predictions for the chemicals BEFORE EXPERIMENT!!!!

Section 2: Work in pairs: To create your pH scale, you will be using solutions 1 to 7 in the table below. You will be using a 12 holed well plate, per row Number each of the wells, use pencil !!!!, to correlate to the number of each of the solutions on the data chart Number 4, red cabbage, is the control What is a control?

Place 3 drops of the cabbage/control into the first 7 labeled wells Put pipettes back into the correct solutions What happens when the pipettes are wrongly placed?

Section 2B: Finish the 5 remaining substances using the same procedure as we did in the initial 7 solutions Use the first 7 solution findings to determine the last 5 solution pH’s Wash well plates extremely thoroughly, why? Stack well plates upside-down and perpendicular to each other

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