Isolation of Mn(II)-oxidizing Bacteria HMC 2010 short presentation Maren Emmerich.

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Presentation transcript:

Isolation of Mn(II)-oxidizing Bacteria HMC 2010 short presentation Maren Emmerich

Mn 2 nd most abundant transition metal in Earth‘s crust Mn redox cycling environmentally important Mystery of microbial Mn 2+ oxidation: Not used for energy metabolism, why else? 3 models of Mn 2+ -oxidizing bacteria: - outer-membrane-associated multicopper-oxidases in Leptothrix discophora & 2 Pseudomonas putida strains - multicopper oxidase in marine Bacillus spores - marine  -proteobacteria Why? How? Results Ideas

Mn 2 nd most abundant transition metal in Earth‘s crust Mn redox cycling environmentally important Mystery of microbial Mn 2+ oxidation: Not used for energy metabolism, why else? 3 models of Mn 2+ -oxidizing bacteria: - outer-membrane-associated multicopper-oxidases in Leptothrix discophora & 2 Pseudomonas putida strains - multicopper oxidase in marine Bacillus spores - marine  -proteobacteria Mn 2+ -oxidizers found in phylogenetically diverse groups Why? How? Results Ideas

I) Sampling at Elkhorn Slough Why? How? Results Ideas II) Streak out 2 x 100 µl on K medium complex medium enriched with 100 µM MnCl 2 III) Aerobic incubation on labbench for 1-3 weeks…

Why? How? Results Ideas Restreak brown colonies

Why? How? Results Ideas Leucoberbelin blue test for MnO 2

Why? How? Results Ideas Some statistics 34 samples from Kirby Park, Strawberry Mat, HMS beach 10 plates with brown colonies after 1-3 weeks (AVG: 1.6) 4 leucoberbelin positives after restreak No isolate yet Successful sampling sites Kirby Park HMS beach

Why? How? Results Ideas Phylogeny of Mn 2+ -oxidizers? Sequence 16S rRNA genes of positive colonies Do isolates grow in liquid? Under which conditions? Inoculate minimal media and add different electron donors & acceptors, incubate +/- light… Energetical benefit of Mn 2+ oxidation? Compare growth yields of isolates grown +/- manganese More ideas? GGggggGg GGggggGg

Thank you!

2H + + 2e - H NAD + + 2H + + 2e - NADH + H S + 2H + + 2e - H 2 S SO H + + 8e - H 2 S pyruvate + 2H + + 2e - lactate FAD + 2H + + 2e - FADH + H cytochrome b (Fe 3+ ) + e - cytochrome b (Fe +2 ) ubiquinone + 2H + + 2e - ubiquinone H cytochrome c (Fe +3 ) + e - cytochrome c (Fe +2 ) NO H + + 2e - NO H 2 O NO H + + 6e - NH Fe +3 + e - Fe O 2 + 4H + + 4e - 2H 2 O Why? How? Results Idea Standard redox potentials at pH = 7

Reaction: Mn O 2 + H 2 O → MnO 2 + 2H + G◦ = −80.8 kJ/mole; Mn E◦ = V

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