University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis: Effect of Calcium Content on Bone Fracture Energy Group Tuesday -

Slides:



Advertisements
Similar presentations
Investigation 11.  Serial dilution  CV will stain skin and clothes  NaOH is a corrosive skin irritant  Wear goggles and aprons  Clean stained glassware.
Advertisements

Department of Bioengineering FRACTURE PROPERTIES OF CHICKEN BONES WITH AND WITHOUT MARROW 101A1 Edwin Akrong – Background & Hypothesis Ping-Chien (Sam)
Spectrophotometers and Concentration Assays
© Sauvé 2002 Sébastien Sauvé Department of Chemistry Université de Montréal Metal speciation using ion-selective electrodes.
CHAPTER 3 DECALCIFICATION Learning Objectives:
Calibration Methods Introduction
Spectrophotometric Analysis of Aspirin
S PECTROPHOTOMETRIC A NALYSIS OF A SPIRIN.  Introduction:  A colored complex is formed between aspirin and the iron (III) ion. The intensity of the.
Making Physical Measurements Terry A. Ring Department of Chemical Engineering University of Utah 22 August 2005.
Department of Bioengineering BRIEF PROJECT TITLE/TOPIC (font 32) Group 101-A2 Joseph Kimmel – Background & Hypothesis Jon Wang – Methods & Protocol George.
Department of Bioengineering Instron Uniaxial Tensile Testing: Mechanical Failure Properties of Suturing and Steri-Strips GROUP #5 Seungyon Kim – Background.
Aims, Objectives and Hypotheses Aims/Objectives Aims/Objectives To determine the effects of surrogate meat (rubber) thickness and loading rate on fracture.
Phosphorus Measurements ä The Technique ä Detection Limits ä Wallastonite ä The Technique ä Detection Limits ä Wallastonite 
Lecture 2b. Electromagnetic Spectrum Visible range: = nm Ultraviolet: = nm Low energyHigh energy.
Testing Effectiveness of Common Antacids
University of Pennsylvania Department of Bioengineering Aims/Objectives: Compare the mass of aspirin between three brands of tablets Use a calibration.
Determination of Iron in Water
Determination of Iron in Water
University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis(es): Quantifying Chicken Bone Decalcification by Mass Section 102 GROUP.
Sulfates Presentation By Douglas Rittmann, Ph.D., P.E. Water/Wastewater Consultant To CE 4153 Lab Course On February 7, 2006.
University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis: Bone Decalcification and Fracture Strength GW4B The objective of this.
Determination of % copper in a Penny
 Decalcification is the process of removal of calcium from decalcified tissue and making suitable for section cutting.  In presence of calcium salts.
Experiment 33: Colorimetric determination of iron
Making Physical Measurements Terry A. Ring Department of Chemical Engineering University of Utah.
Decalcification. Decalcification are the most type here, but other tissues may contain calcified areas as well. Bone specimens are the most type here,
Dilution and Spectroscopy Lab Lab Word Document Lab Word Document.
SPECTROPHOTOMETRY IN BIOTECHNOLOGY
Spectrophotometers and Concentration Assays
Exp. 18: Spectrophotometric Analysis: Concentration of a Solution Using Beer’s Law White light violet (400nm) – red (800nm) “visible spectrum” sample (light.
An Introduction to the Spectrophotometer. Meet your Spectrophotometer Meet your spectrophotometer.
University of Pennsylvania Department of Bioengineering Objectives & Hypotheses: Analysis of the Effect of Calcium Content on Chicken Bone Fracture Energy.
University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis(es): MATERIAL TESTING: ROCKWELL HARDNESS & IMPACT ENERGY Group 101B5.
Department of Bioengineering Effect of Decalcification on Fracture Strength in Chicken Bones Group 102A5 Tim Bakke Vincent Cina Jonathan Lee Wee Siong.
Student Directed Investigation – due dates. Due Dates February 13 – Methods & Materials Section due (in Google drive – shared with me) February 13 – Introduction.
University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis: Fracture Energy of De-calcified Chicken Bones Group WA2 Central Hypothesis:
University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis: SPECTROANALYSIS OF ACETYLSALICYLIC ACID CONTENT IN ASPIRIN - TB4 The.
Effect of pH on Aspirin Dissolution
Department of Bioengineering Buffering Capabilities of Guinea Pig Blood GROUP MB3 Kim Coughlan, Barry Huang, Laura Michelis, Le Truong.
Department of Bioengineering Bone Repair Techniques: Fracture Testing of Chicken Bones 102-1B Ted Lee – Background & Hypothesis Nikhil Deshmukh – Methods.
Department of Bioengineering Tensile Properties of Chicken Skin Treated with Trypsin-EDTA Group 3A Sakil Chundydyal – Background & Hypothesis Arnav Mukherjee.
University of Pennsylvania Department of Bioengineering Objectives & Hypotheses: REINFORCED BONE SURROGATE FRACTURING 101-A3 Objectives: To determine the.
Lab Activity 10 Purification of LDH from Chicken Part I IUG, Fall 2012 Dr. Tarek Zaida IUG, Fall 2012 Dr. Tarek Zaida 1.
Department of Bioengineering SUTURE PERFORMANCE 102 A Gyong Min Bak – Background & Hypothesis Heather Forquer – Methods & Protocol Kelvin Leung – Deliverables.
Experiment 33 Colorimetric Determination of Iron CHE1181.
University of Pennsylvania Department of Bioengineering Objective To even force distribution over all stitches and thus increase force needed for system.
COLORIMETRY & SPECTROPHOTOMETR
Spectrophotometers and Concentration Assays Chapter 7.
Applying Neutralization Titrations.
Experiments in Analytical Chemistry -EDTA determination of Ca and Mg in water.
Protocol: Isolation of proteins Determination of protein concentration
Colorimetric Determination of Iron
Making Physical Measurements
Crystal Violet Lab Investigation 11.
Fracture Energy of Chicken Bones:
Determination of Protein Concentration of a Solution
iCAP OES Analysis of Trace Elements in Hair
Making Physical Measurements
Vernier Spectrophotometer
Lab Activity 11 Purification of LDH from Chicken Part I
Impact of Stitch Density on Suture Performance
Bone Density and Impact Energy
Colorimetric Determination of Keq
Protocol: Isolation of proteins Determination of protein concentration
Exp. Iron in Vitamin Tablet
Quantitative tests for proteins
Quantitative tests for proteins
Lab Activity 10 Purification of LDH from Chicken Part I
Quantitative Proteins Estimation by lowry method
Spectrophotometry A method to determine concentration of a species exploiting the absorption of EMR.
Presentation transcript:

University of Pennsylvania Department of Bioengineering Aims/Objective & Hypothesis: Effect of Calcium Content on Bone Fracture Energy Group Tuesday - A1 The primary objectives of this experiment are to determine the calcium content via chemical extraction of calcium from 10 chicken bone samples and investigate the relationship between the calcium concentration in the bone samples and the associated energy required to fracture the bones, which will be determined through the pendulum fracture technique. Hypothesis: There is a positive linear correlation between the amount of calcium present in the chicken bones and bone fracture strength

University of Pennsylvania Department of Bioengineering Methods, Protocol & Equipment: 1.Fracture bone using same methods used in the Impact Pendulum Testing Lab 1.Cut off section of fractured bone near point of fracture 2.Wash out bone section with distilled water in order to discard bone marrow 2.Perform surface decalcification technique 1.Prepare chelating agent using 31.2 g NaH 2 PO 4, 28.4 g Na 2 HPO 4, 140 g EDTA and 2 L distilled water 2.Cut bone section into thin, flat (~1 g) sections for rapid decalcification 3.Suspend thin sections in 50 mL of chelating agent for 15 minutes 3.Perform colorimetric analysis of 3.5 mL samples of the decalcified solution 1.Stain decalcified solution with calcium detection reagent 2.Place stained solution in cuvette and measure absorbance using a spectrophotometer at 550 nm 3.Compare absorbance value to calibration curves in the calcium detection reagent literature EDTA

University of Pennsylvania Department of Bioengineering Proposed Results/Findings/Deliverables: Using the chemical extraction of calcium and the subsequent determination of calcium concentration via colorimetric analysis, the calcium content in bone samples will be calculated. Due to the fact that calcium is present in significant amounts in bone, there presumably exists a positive linear relationship between calcium content and the strength of the bone (energy required to fracture the bone.)

University of Pennsylvania Department of Bioengineering Potential Pitfalls: Methods Pitfall What if the Ca 2+ content in the small bone fragments is too small for the colorimetric analysis to analyze? EDTA too slow for six hour lab. Chromogenic tetracarboxylic acid may be used instead (2 min rxn time) Will we be able to back calculate meaningful Ca 2+ measurements when using such small concentrations? May have to increase specimen size and/or increase sample size (n). Statistics Pitfall What if there is no significant difference between Ca 2+ content in the different chicken specimens? Observed in the cross-sectional areas and bone masses of pilot lab Perform completely different test: Find calibration curve of strong decalcifying agent on known calcium tablet over time (% Ca 2+ lost vs. time) Do pre-hoc test on bones and then fracture Determine effect of time in EDTA on fracture strength Reliable results

University of Pennsylvania Department of Bioengineering Materials and Budget & Justification: Necessary Materials:Price ($): 1kg Sodium Orthophosphate g O-cresolpthalein mg EDTA Surgical Blades (10) Blade Holders (4) mg Calcium Tablets (140) Chicken (2-8 pack) 5.00 Total: $ Materials Already Available: Pendulum Apparatus Spectrophotometer Glassware