SHC p13.3 SHC (Src homology 2 domain containing) transforming protein 2 CDC p13.3cell division cycle 34 GZMM p13.3granzyme M (lymphocyte met-ase 1) BSG p13.3basigin (Ok blood group) FGF p13.3fibroblast growth factor 22 RNF p13.3ring finger protein 126 FSTL p13follistatin-like 3 (secreted glycoprotein) PTBP p13.3polypyrimidine tract binding protein 1 AZU p13.3azurocidin 1 PRTN p13.3proteinase 3 ELANE p13.3elastase, neutrophil expressed KISS1R p13.3KISS1 receptor ARID3A p13.3AT rich interactive domain 3A (BRIGHT-like) TMEM p13.3transmembrane protein 259 HMHA p13.3histocompatibility (minor) HA-1 POLR2E p13.3 polymerase (RNA) II (DNA directed) polypeptide E, 25kDa GPX p13.3glutathione peroxidase 4 STK p13.3serine/threonine kinase 11 CIRBP p13.3cold inducible RNA binding protein EFNA p13ephrin-A2 MUM p13.3melanoma associated antigen (mutated) 1 DAZAP p13.3DAZ associated protein 1 RPS p13.3ribosomal protein S15 APC p13.3adenomatosis polyposis coli 2 PCSK p13.3proprotein convertase subtilisin/kexin type 4 REEP p13.3receptor accessory protein 6 PLK p13.3polo-like kinase 5 MEX3D p13.3mex-3 homolog D (C. elegans) MBD p13methyl-CpG binding domain protein 3 TCF p13.3transcription factor 3 ATP8B p13.3 ATPase, aminophospholipid transporter, class I, type 8B, member 3 CSNK1G p13.3casein kinase 1, gamma 2 BTBD p13.3 -p12 BTB (POZ) domain containing 2 MKNK p13.3MAP kinase interacting serine/threonine kinase 2 AP3D p13.3adaptor-related protein complex 3, delta 1 subunit DOT1L p13.3DOT1-like histone H3K79 methyltransferase AMH p13.3anti-Mullerian hormone OAZ p13.3ornithine decarboxylase antizyme 1 SPPL2B p13.3signal peptide peptidase like 2B TIMM p13.3 translocase of inner mitochondrial membrane 13 homolog (yeast) GADD45B p13.3growth arrest and DNA-damage-inducible, beta GNG p13.3 guanine nucleotide binding protein (G protein), gamma 7 DIRAS p13.3DIRAS family, GTP-binding RAS-like 1 SLC39A p13.3 solute carrier family 39 (zinc transporter), member 3 SGTA p13 small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha TLE p13.3 transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) AES p13.3amino-terminal enhancer of split GNA p13.3 guanine nucleotide binding protein (G protein), alpha 11 (Gq class) GNA p13.3 guanine nucleotide binding protein (G protein), alpha 15 (Gq class) S1PR p13.3sphingosine-1-phosphate receptor 4 NFIC p13.3 nuclear factor I/C (CCAAT-binding transcription factor) DOHH p13.3deoxyhypusine hydroxylase/monooxygenase FZR p13.3fizzy/cell division cycle 20 related 1 (Drosophila) HMG20B p13.3high mobility group 20B TBXA2R p13.3thromboxane A2 receptor CACTIN p13.3cactin, spliceosome C complex subunit PIP5K1C p13.3 phosphatidylinositol-4-phosphate 5-kinase, type I, gamma MATK p13.3megakaryocyte-associated tyrosine kinase NMRK p13.3nicotinamide riboside kinase 2 DAPK p13.3death-associated protein kinase 3 MIR p13.3microRNA 637 EEF p13.3eukaryotic translation elongation factor 2 PIAS p13.3protein inhibitor of activated STAT, 4 ZBTB7A p13.3zinc finger and BTB domain containing 7A MAP2K p13.3mitogen-activated protein kinase kinase 2 SIRT p13.3sirtuin 6 EBI p13Epstein-Barr virus induced 3 List of genes in human melanoma 19p13.3 focal deletion Liu et al. Supplementary Figure S1

Rank 19p13.3 Gene Name Gene DescriptionProbe Fold Chan ge P Value 1CFD complement factor D (adipsin)205382_s_at E GNA1 5 guanine nucleotide binding protein (G protein), alpha 15 (Gq class)205349_at E STAP 2 signal transducing adaptor family member _s_at E -09 4INSRinsulin receptor213792_s_at E SH2D 3A SH2 domain containing 3A219513_s_at E MKN K2 MAP kinase interacting serine/threonine kinase _s_at ZBTB 7A zinc finger and BTB domain containing 7A219186_at E -07 Rank 19p13. 3 Gene Name Gene DescriptionProbe Fold Chang e P Value 1C3 complement component _at E- 19 2CFD complement factor D (adipsin)205382_s_at E GADD 45B growth arrest and DNA- damage- inducible, beta207574_s_at ELANE elastase, neutrophil expressed206871_at GADD 45B growth arrest and DNA- damage- inducible, beta209304_x_at ZBTB7 A zinc finger and BTB domain containing 7A213303_x_at ANGP TL4 angiopoietin- like _s_at A B Talantov dataset Kabbarah dataset Liu et al. Supplementary Figure S2

Ran kProbe set Gene Symbolfold changeP value _atNRXN _a_atMCAM _atICAM _atNRXN _atCD8B _atCD _atCD _atCD _a_atSDC _atNCAM _atNLGN _atJAM _atPTPRF _atNRXN _s_atPVR _atJAM _atPVR _atSELP _atNRXN A B * * * * * RankNAMESIZENESFDR q-val 1KEGG_GALACTOSE_METABOLISM KEGG_CELL_ADHESION_MOLECULES_CA MS KEGG_FRUCTOSE_AND_MANNOSE_MET ABOLISM KEGG_ALLOGRAFT_REJECTION KEGG_STARCH_AND_SUCROSE_METAB OLISM KEGG_LYSOSOME KEGG_EPITHELIAL_CELL_SIGNALING_IN_ HELICOBACTER_PYLORI_INFECTION KEGG_GRAFT_VERSUS_HOST_DISEASE KEGG_OTHER_GLYCAN_DEGRADATION KEGG_BLADDER_CANCER KEGG_SYSTEMIC_LUPUS_ERYTHEMATO SUS KEGG_AUTOIMMUNE_THYROID_DISEASE KEGG_ARACHIDONIC_ACID_METABOLIS M KEGG_AMINO_SUGAR_AND_NUCLEOTID E_SUGAR_METABOLISM KEGG_DORSO_VENTRAL_AXIS_FORMATI ON KEGG_HYPERTROPHIC_CARDIOMYOPAT HY_HCM KEGG_DILATED_CARDIOMYOPATHY KEGG_VIBRIO_CHOLERAE_INFECTION KEGG_INSULIN_SIGNALING_PATHWAY Liu et al. Supplementary Figure S3

Con Zbtb7a Δ/Δ Mcam Mcam Zbtb7a Mcam Hsp90 Zbtb7a Primary MEF Transformed MEF Microarray data Rt-PCR Con Zbtb7a Δ/Δ A B P=1x10 -6 P=1x10 -5 P=0.01 Relative mRNA expression Liu et al. Supplementary Figure S4

MCAM Prognosis A B Primary melanoma Metastasis melanoma MCAM CNV Survival probability Time (Days) High MCAM expression Low MCAM expression n. s. P=0.005 Relative CNV value Liu et al. Supplementary Figure S5

(Human) (Mouse) Sequence alignment between human and mouse MCAM promoter A E D FLAG-ZBTB7A ZBTB7A  -actin B FLAG-ZBTB7A Con Con ZBTB7A P=0.002P= % input MCAM promoter primer 2# MCAM promoter primer 3# Liu et al. Supplementary Figure S6 Conserved ZBTB7A binding sites in human MCAM promoter Luciferase/Renilla pGL3 (basic) pGL3-MCAM ZBTB7A -- Luciferase/Renilla ZBTB7A -- C P= P= P=0.0001