bioplastic from waste 1confidential Ryan Smith, CTO

Micromidas converts biological waste into biodegradable plastic 2confidential

Micromidas converts biological waste into biodegradable plastic 3confidential 132

Jackson

Annually, 150 million tons of plastic is thrown “away”

PHA (Poly-hydroxyalkanoates) a family of linear aliphatic polyesters In the environment PHAs are biodegraded by many species of bacteria. PHA

Plastic requires 8% of the world’s oil

PHA granules in bacteria Poly-hydroxyalkanoates (PHAs) are naturally synthesized biological polyesters. They serve as a carbon energy storage compound for bacteria and are stored as intracellular granules.

Feedstock Landscape New is as bad as the Old

Feedstock Landscape 10confidential

Wastewater Solids Sludge is a by-product of treatment Solids Disposal is 40% of treatment cost 8,200,000 MT/year in US Treatment Cost: $ /MT Sludge capacity limited Increasingly regulated Actively seeking new treatment options 11confidential

Inflow (100%) Primary Solids Primary Effluent Biosolids (43%) Bio Gas (21%) CO 2 (33%) Secondary Effluent (3%) Activated Sludge Digestion 2 o Clarifier 1 o Clarifier Aeration Pond Relative Carbon Flow through Wastewater treatment plant (WWTP)

Inflow (100%) Primary Solids Primary Effluent Residual Solids (32%) Lignin, Pulped Cellulose, Inorganic Salts, Silicates Bio Gas (3%) Soluble Carbon CO 2 (18%) PHA (9%) Cells (3%) CO 2 (33%) Secondary Effluent (3%) Activated Sludge Micromidas Acid Phase Digestion 2 o Clarifier 1 o Clarifier Aeration Pond Relative Carbon Flow through Micromidas integrated WWTP

Technology and Tools 14confidential Techalive.mtu.edu Hiwtc.com Wastewater Style Processing Low Cost HIGH VOLUME Existing Operators Same Equipment Infrastructure in place Very Robust

15 confidential 2010.igem.org/Team:INSA-Lyon/Project/Stage2/Theory Selected bioprospecting Source Microbes Soil Aerobic Treatment Pond Water Evolve Basic Selection Genetic 100 ml growth 100 ml production Resist contamination Evaluation Process Metabolic Ralstonia Eutropha H16 Pseudomonas putida KT 2440 Bacillus cereus Productive Strains

Microbe Library DEA-003

Micromidas was formed in 2008 and is currently working with several wastewater treatment facilities across the world to help address problems of costly bio-solids removal. Micromidas developed a fermentation technology that partitions the organic nutrients from waste and then utilizes a robust set of organisms to store the energy from those nutrients as intracellular bio-plastic.

18confidential

bioplastic from waste 19confidential Ryan Smith, CTO