Enzymes: Molecules That Speed Up Reactions. What are Enzymes Enzymes are proteins that speed up the rate of all reactions. Also know as Biological Catalysts.

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Presentation transcript:

Enzymes: Molecules That Speed Up Reactions

What are Enzymes Enzymes are proteins that speed up the rate of all reactions. Also know as Biological Catalysts. Enzymes lower the Activation Energy, which is the energy required to break down existing bonds between atoms Enzymes speed up two types of reactions: 1.Exergonic Reactions 2.Endergonic Reactions

Enzyme 3D Molecular Model Active site (a) (b)

How Enzymes Work Every cell carries out many thousands of metabolic reactions per second. Each reaction starts with Reactants (molecules undergoing the reaction) and ends up with a Product or Products. Reactants (also known as Substrates) bind to the Active Site of the enzyme and form an Enzyme-Substrate Complex. At this time reactants undergo chemical changes that involve the “making or breaking” bonds Then reactants are transformed into products

Enzymes at Work

Enzyme Reactions Enzymes speed up two types of reactions: 1.Exergonic Reactions: Bond Breaking 2.Endergonic Reactions: Bond Making

Exergonic Reactions Reactions that break bonds (bond breaking) Release energy stored within the bonds Energy is Released

Endergonic Reactions Reactions that build bonds (bond making) Require the input of energy to make bonds Energy is Required

Factors Affecting Enzymes 1.Enzyme Concentration The total amount of enzyme present at the time of the reaction 2.Temperature Enzymes work best at an optimum temperature Most enzymes in humans work best at 37ºC 3.pH Most enzymes are effective only in a narrow pH range Enzymes work best at on optimum pH

Factors Affecting Enzymes 4. Cofactors: Substances that bind to enzymes and help enzymes work best Organic Cofactors: Vitamins and other compounds made from vitamins. Organic cofactors are alos known as coenzymes Ex: Vitamins A, B, D, E, K, etc. Inorganic Cofactors: Metal atoms such as Copper, Zink, Magnesium, Iron, etc. 5. Inhibitors: Substances that interfere with enzymes, reducing or even destroying the enzyme activity Ex: Cyanide, poisons, pesticides, painkillers, etc

Figure 6-12 Page 132 Most human enzymes Pepsin Trypsin Enzymes of heal-tolerant bacteria pH Rate of reaction Temperature (˚C) (a) (b)

Rate of reaction Enzyme concentrationSubstrate concentration (a) (b)