Light Microscopy Bright Field Fluorescence Phase-Contrast Differential Interference Contrast (DIC)

Slides:

Advertisements

Similar presentations

The process of making proteins

Advertisements

Both are nucleic acids… Be able to compare these two nucleic acids.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Central Dogma Big Idea 3: Living systems store, retrieve, transmit, and respond to info essential to life processes.

RNA and Protein Synthesis

Chapter 10 Gene Action: From DNA to Protein

Simultaneous transcription and translation in prokaryotes Green arrow = E. coli DNA Red arrow = mRNA combined with ribosomes.

Protein Synthesis Mrs. Harlin.

Summary Structure of DNA (sugar-phosphate backbone, base pairs, double helix) RNA Structure (types, how is it different from DNA?) Transcription (RNA.

Colinearity of Gene and Protein DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription translation.

Fig Pg. 25 Inherited Disorders “Sporadic” Cancers Aging Lysosomal Storage diseases I-Cell Disease Cystic Fibrosis Cholera “Sporadic” Cancers Aging.

Structure and Study of Macromolecules

PROTEIN SYNTHESIS BY: MARIAH GUMFORY. OBJECTIVES Explain the purpose and process of transcription and translation Recognize that gene expression is a.

Protein Synthesis and Structure Section 2-4. Protein Functions: General Information Proteins account for almost 50% of the dry mass of most cells Proteins.

Chapter 13.2 (Pgs ): Ribosomes and Protein Synthesis

Quiz tiiiiime What 3 things make up a nucleotide?

Protein Synthesis (Eukaryotes)

NUCLEIC ACIDS AND PROTEIN SYNTHESIS. QUESTION 1 DNA.

RNA processing in eukaryotes

Chapter 17 Transcription and Translation Dr. Joseph Silver.

RNA & Protein Synthesis. I. DNA to Genes A. We now know how the double helix is replicated but we still don’t know how it is then transformed into genes.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Figure 17.0 Ribosome. Figure 17.1 Beadle and Tatum’s evidence for the one gene-one enzyme hypothesis.

12-3 RNA and Protein Synthesis

Genetics AP Biology. The Discovery of DNA Structure Rosalind Franklin: x-ray diffraction photographs of DNA Rosalind Franklin: x-ray diffraction photographs.

Review of Protein Synthesis. Fig TRANSCRIPTION TRANSLATION DNA mRNA Ribosome Polypeptide (a) Bacterial cell Nuclear envelope TRANSCRIPTION RNA PROCESSING.

PROTEIN SYNTHESIS The Blueprint of Life: From DNA to Protein.

Protein Synthesis. Transcription DNA  mRNA Occurs in the nucleus Translation mRNA  tRNA  AA Occurs at the ribosome.

Human Anatomy & Physiology FIFTH EDITION Elaine N. Marieb PowerPoint ® Lecture Slide Presentation by Vince Austin Copyright © 2003 Pearson Education, Inc.

Cell Protein Production. Transcription : process of mRNA formation. 1. Triggered by chem. messengers from cytoplasm which bind to DNA 2. This causes release.

Pre – AP Biology Protein Synthesis (4.2) Part 1. George Beadle & Edward Tatum.

CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company.

Functions of RNA mRNA (messenger)- instructions protein

Exam #1 is T 2/17 in class (bring cheat sheet). Protein DNA is used to produce RNA and/or proteins, but not all genes are expressed at the same time or.

Gene Expression Gene: contains the recipe for a protein 1. is a specific region of DNA on a chromosome 2. codes for a specific mRNA.

Protein Synthesis “From code into Flesh & Blood”.

DAY 2. Warm Up What type of RNA copies DNA? – mRNA What is this process called? – Transcription.

Transcription & Translation Molecular Structure of Ion Channels.

C Morritt Protein Synthesis Starfish Text pages 472 to 481.

RNA processing and Translation. Eukaryotic cells modify RNA after transcription (RNA processing) During RNA processing, both ends of the primary transcript.

1 From Bi 150 Lecture 0 October 4, 2012 An introduction to molecular biology... but you will learn the cell biology in this course.

Transcription and Translation

From Gene to Protein Chapter 17. Overview of Transcription & Translation.

PROTEINS. LIFE IS AN ORGANIZED SYSTEM OF COMPLEX CHEMICAL REACTIONS.

Genetic Code and Interrupted Gene Chapter 4. Genetic Code and Interrupted Gene Aala A. Abulfaraj.

NOTES CHPT. 10.

Lecture 13 :Protein synthesis TRANSLATION

Lecture 13 :Protein synthesis TRANSLATION

Transcription and Translation

Gene Expression: from DNA to protein

Exam #1 W 9/26 at 7-8:30pm in UTC 2.102A Review T 9/25 at 5pm in WRW 102 and in class 9/26.

An introduction to molecular biology

Cytosol Nucleus Mitochondria Secreted ER Golgi Endosome Lysosome Transmembrane.

Protein Synthesis.

RNA and Protein Synthesis

Protein Synthesis Ch 17.

RNA and Protein Synthesis

Protein Synthesis.

Protein Synthesis- the "STuff of Life".

DNA & Protein Synthesis Task Cards

Protein Synthesis in Detail

The Central Dogma of Life.

Translation and Point Mutations

Eukaryote Regulation and Gene Expression

Pg. 10. Pg. 10 Microscopy Light Microscopy Electron Microscopy.

RNA and Protein Synthesis

Lecture 13 :Protein synthesis TRANSLATION

Pre – AP Biology Protein Synthesis.

From gene to protein.

Cell protein production

Presentation transcript:

Light Microscopy Bright Field Fluorescence Phase-Contrast Differential Interference Contrast (DIC)

Electron Microscopy (EM) Scanning EM Transmission EM

Chemical Composition of Cells Pg. 58

Pg. 79

Chemical Bond Energies

Gene Expression

Monomer/Polymer

Nitrogenous Bases of DNA and RNA Pg. 76

Pg. 175

3’ 5’

0.34 nm Pg. 171 Major Groove Minor Groove

Supercoiled DNA Non-supercoiled DNA (relaxed)

Werner Syndrome 15 yrs. 48 yrs. Mutation in gene encoding RecQ helicase

Pg. 175

3’ 5’

Human Karyotype Pg. 179

RNA Molecules 5’ 3’

Pg. 74

(Pgs ) Amino Acid Side Chains

Extra carbon atom!

ß-Sheet alpha-helix

Hydrophobic Interactions in Tertiary Structure

Representations of Protein Tertiary Structure Backbone Ribbon Wire Frame Space Filling Pg. 132

Quaternary Structure Pg. 141

Chloride Ion Channel Protein

Comparison of Protein Primary Structure Cyclin-dependent Kinase Proteins

Gene Expression Pg. 230

Pg. 202 RNA Synthesis OH

Pg. 233

1 µm Pg. 233

Fig. 7-20

Exon/Intron Organization of Eukaryotic Genes Pg. 238

Sequence Structure of Introns

Pg. 239

Pg. 241 Alternative Splicing

Pg ’ Guanine Cap Structure

Cytosol Nucleus Mitochondria Secreted ER Golgi Endosome Lysosome Transmembrane

Reading Frames in RNA

tRNA Synthetase tRNA Amino Acid Synthetase (blue) Editing Domain

“Editing” by tRNA Synthetase

Large Subunit RNA Secondary Structure

Small Ribosomal Subunit Pg. 253

3’ mRNA Structure

Pg. 253 Initiation of Translation

Pg. 252

tRNA Release Factor (RF)

Co-translational Protein Folding

Protein Folding by “Chaperones”

Transcriptional Activator Transcriptional Repressor

Pg. 257 Ubiquitin-dependent Protein Degradation Proteasome Amino acids + Ub Protein Ub-Ub-Ub Protein + Ub Ubiquitin Ligase

Coding and catalysis Coding Coding intermediate

Superficial Infections Systemic Infections Fungal Disease

Onochomycosis

AN2690 Structure

Figure 1A

Figure 1B

Figure 3 AN2690