“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system Thank you. Nucleeases will be used as a new tool that.

Slides:

Advertisements

Similar presentations

Hot New Technologies CRISPR-Cas CRISPR – clustered regularly interspersed short palindromic repeat Cas9 - CPISPR-associated Very new Edit or.

Advertisements

Genome Editing Dieter Steinmetz ZMBP Universität Tübingen Kurs SS 2015.

Targeted Cancer Therapy Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520.

The case:  2005: No production Continued sampling  2006: Detected plants expressing transgene - demonstrated pollen transfer and seed dispersal (Reichman.

1 Jin-Soo Kim Dept. of Chemistry, Seoul National Univ. Center for Genome Engineering, Institute for Basic Science Genome Editing: Challenges and Opportunities.

Fig. 1 Timeline of CRISPR-Cas and genome engineering research fields

Lecture-7 Genome editing CRISPR

Genome Editing Katie Plummer.

Maimona Saeed Ph. D Scholar Department of Botany

National Postdoc Appreciation Week

CRISPR BTE 302 FINAL PRESENTATION.  Ehsan Sakib ( )  Prateem Das ( )  Olia (136032)

Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520

Genome editing to breed better plants

CRISPR is cheap, easy to do, and powerful… is it also dangerous?

Use of CRISPR/Cas to edit the Arabidopsis Na+/H+ Antiporter NHX1

Institute of Health and Biomedical Innovation

Genome Editing AGRY 600 Fall 2016.

Alexander Kena, Heng Ye, Jiuhuan Feng, Xingyou Gu

CRISPR-Cas9 in development and genetic disease

CRISPR Cas9 Genome Editing in Germlines

Lecture – FALL 2017 Modern methods in Molecular Biology

Assessing the role of the ALS-associated gene NEK1 in zebrafish motor neuron development Amy Stark.

Expanding the Biologist’s Toolkit with CRISPR-Cas9

Reminders Unit 7 Exam- Friday, February, 24th

CRISPR + CAS = Defensive or Immune System

Dieter Steinmetz Universität Tübingen – ZMBP Kurs WS 15/16

CRISPR/Cas9 Clustered Regularly Interspaced Short Palindromic Repeats

CRISPR/Cas9 GENOMICS Lucía Álvarez González

Genome-editing Technologies for Gene and Cell Therapy

Nat. Rev. Rheumatol. doi: /nrrheum

In vivo optimization of the tagging approach using the Act5C model locus and flow cytometry-based quantification of the Act5C-GFP tagging success. In vivo.

BPTF role in PI3K-AKT pathway in breast cancer

Figure 2 Use of CRISPR/Cas9 for genome editing

Genomics for Regional Development

Molecular Therapy - Nucleic Acids

Harnessing CRISPR–Cas systems for bacterial genome editing

Astrid Breitbart, Charles E. Murry Cell Stem Cell

Second International Summit on Human Genome Editing

The CRISPR/Cas9 system.1 Clustered regularly interspaced palindromic repeats (CRISPR) refers to sequences in the bacterial genome. The CRISPR/Cas9 system.1.

Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology Joan Ramon Guitart, Jodi L. Johnson,

Feasibility of new breeding techniques for organic farming

Figure 1 Adaptive immune system of bacteria and archaea

Astrid Breitbart, Charles E. Murry Cell Stem Cell

Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR

CRISPR-Cas9 for in vivo Gene Therapy: Promise and Hurdles

The CRISPR/Cas9 system.1 Clustered regularly interspaced palindromic repeats (CRISPR) refers to sequences in the bacterial genome. The CRISPR/Cas9 system.1.

Joseph Bondy-Denomy, Alan R. Davidson Trends in Microbiology

CRISPR genome-editing: A medical revolution

Functional analysis of CMYA5 via CRISPR/Cas9 knockout

Molecular Therapy - Nucleic Acids

RNAi: Prokaryotes Get in on the Act

CRISPR: What is it? Biotech Ethics, Fall ‘18.

Current Progress in Therapeutic Gene Editing for Monogenic Diseases

Genome-editing Technologies for Gene and Cell Therapy

William T. Hendriks, Curtis R. Warren, Chad A. Cowan Cell Stem Cell

Gene editing - CRISPR/Cas9 System

Gene editing: modifying a gene that was already there

Scholarship Symposium

Gene editing by CRISPR/Cas9 for gene inactivation and targeted sequence replacement. Gene editing by CRISPR/Cas9 for gene inactivation and targeted sequence.

Knockdown of endogenous WT-hTER by a short hairpin RNA (siRNA) directed specifically against the human WT telomerase RNA template. Knockdown of endogenous.

Chemical Biology Approaches to Genome Editing: Understanding, Controlling, and Delivering Programmable Nucleases Johnny H. Hu, Kevin M. Davis, David R.

MMEJ plays a dominant role in double-strand DNA break repair in L

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off- target Effects in Genome Editing Ciaran M Lee, Thomas J Cradick,

A, top, representative Western blot for Ku70 in MCF7 cell extracts following siRNA knockdown of Ku70, compared with siRNA control. A, top, representative.

CRISPR Craze to Transform Cardiac Biology

Production of ade2Δ/ade2Δ mutants by the Candida CRISPR system.

Presentation transcript:

“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system Thank you. Nucleeases will be used as a new tool that may revolutionize how we study genes. Story of growing up on the farm. Scientific discovory can be limited be the tools available. Next few slides are a history of the DNA discoveries and tools used to manipulate DNA Jason Kerkvliet, M.S. Research Associate II Hoppe lab March 4, 2014

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system

DNA repair mechanisms: gene editing efficiency is enhanced by creating breaks in DNA

How does one use CRISPR? For NHEJ CRISPR vector $65 4 (2 sgRNA sequences) oligos $40 BbsI $70 Total = $175

How does one use CRISPR? For HDR

CRISPR gRNA design tool crispr.mit.edu

CRISPR/Cas9 can be used to edit more than one gene in parallel Wang et al., Cell, 2013

Gene knock-down (siRNA) versus gene knock out Shalem et al., Science 2014

Eliciting gene function using a CRISPR mediated genome screen Shalem et al., Science 2014

http://www.addgene.org/CRISPR/

http://www.addgene.org/CRISPR/plant/