S1. MCF7 M (high density) Day 3 Day 5 S2 AB S3 S4 NetworkspValueGenes from Active Data Chemotaxis7.991E-11IL-1 beta, CCR2, GRO-3, Lck, CCL13, Galpha(q)-specific.

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Presentation transcript:

S1

MCF7 M (high density) Day 3 Day 5 S2

AB S3

S4 NetworkspValueGenes from Active Data Chemotaxis7.991E-11IL-1 beta, CCR2, GRO-3, Lck, CCL13, Galpha(q)-specific peptide GPCRs, Galpha(i)-specific peptide GPCRs, SDF-1, IL- 16, CCL7, GRO-2, PD-ECGF (TdRPase), GCP2 Proteolysis_ECM remodeling1.820E-09MMP-13, MMP-14, TIMP3, MMP-1, TIMP4, MMP-15, MMP- 24, MMP-1, MMP-25, Stromelysin-1 Inflammation_Jak-STAT pathway 4.161E-09IL21R, CCR2, PDGF-R-beta, IL5RA, CCL13, PDGF receptor, IL- 2R gamma chain, IL-21 receptor, SDF-1, IL10RA, IGF-1, CCL14, IL-20 Proteolysis_Connective tissue degradation 4.951E-08MMP-13, MMP-14, TIMP3, MMP-19, TIMP4, MMP-15, MMP- 24, MMP-1, MMP-25, Stromelysin-1 Cell adhesion_leukocyte chemotaxis 9.863E-07CCR2, GRO-3, Lck, CCL13, Galpha(q)-specific peptide GPCRs, Galpha(i)-specific peptide GPCRs, SDF-1, CCL14, CCL7, GRO-2, GCP2 Immune response_Th17- derived cytokines 1.520E-06IL-1 beta, MMP-13, SDF-1, CCL7, RANKL(TNFSF11), GCP2, MMP-1, Stromelysin-1 Development_Blood vessel morphogenesis 2.810E-06IL-1beta, FGF8, Galpha(q)-specific peptide GPCRs, Galpha(i)- specific peptide GPCRs, MMP-19, SDF-1, ALK-2, VEGFR-2, TIE, PD-ECGF (TdRPase), VEGF-D Cell adhesion_Platelet- endothelium-leukocyte interactions 1.497E-05IL-1 beta, MMP-13, PDGF-R-beta, Endoglin, PDGF receptor, VEGFR-2, Thrombospondin 4, TGF-beta receptor type II, PDGF-R-alpha Apoptosis_Anti-Apoptosis mediated by external signals via PI3K/AKT 2.345E-05IL-1 beta, FGF8, PDGF-R-beta, PDGF receptor, GFRalpha1, IGF-1, VEGFR-2, NT-3, VEGF-D, PDGF-R-alpha

S5 DatasetNumber of patients Number in patient subset Comparison Scheutz et al14 DCIS vs IDC Ma et al (Ref 32) 6620Epithelium vs Stroma Boersma et al9583Epithelium vs Stroma

S6

S MCF-7 M (high) + E 2 MCF-7 M (high) + Veh MCF-7 M (low) + E 2 MCF-7 M (low) + Veh MCF-7 + Veh MCF-7 + E 2 Absorbance Wavenumber (cm -1 )

S MCF-7+ Veh MCF-7 + E 2 MCF-7+CM + Veh MCF-7+ CM E 2 Absorbance Wavenumber (cm -1 )

S9 MCF-7MCF-7 + HMF