1234M 36 kDa 28 55 95 130 5 Supplementary Fig. 1 Supplementary Fig. 1. Expression and purification of Bacillus sp. BRC1 Bdh in/from E. coli. Lane 1, total.

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1234M 36 kDa Supplementary Fig. 1 Supplementary Fig. 1. Expression and purification of Bacillus sp. BRC1 Bdh in/from E. coli. Lane 1, total cell lysates of E. coli BL21 harboring pET28a (control); lane 2, total cell lysates of E. coli BL21 harboring pET-bhd; lane 3, soluble fraction; lane 4, insoluble fraction; lane 5, purified Bdh (arrow).

A B Supplementary Fig. 2 Supplementary Fig. 2. Effects of pH (A) and temperature (B) on Bdh activity toward the substrates D-2,3- BD (closed circles) and acetoin (open circles).

A C B D Supplementary Fig. 3 Supplementary Fig. 3. Enzyme kinetics of Bacillus sp. BRC1 Bdh. A, D-2,3-BD; B, Meso-2,3-BD; C, Acetoin; D, Diacetyl

BRC1/pWHBRC1/pWH-bdh Xylose 5% Xylose 20% EFB hydrolysate Supplementary Fig. 4 Supplementary Fig. 4. Enzyme activity of Bdh from recombinant Bacillus sp. BRC1 strains. Closed bars, D- 2,3-BD; open bars, acetoin.