Lecture 10 – Second Messengers IV – Niric Oxide, cGMP, and ROS Signalling BIOL 5190/6190 Cellular & Molecular Singal Transduction Prepared by Bob Locy Last modified -13F

CSB Fig 02:01 Cell signalling pathways CSB Fig 02:01 Cell signalling pathways Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge

Transducers Second Messengers II 1.Nitric Oxide cyclic GMP Signaling cyclic GMP Signaling 2.Redox signaling 3.ROS signaling

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:45 NO and cyclic GMP signalling CSB Fig 2:45 NO and cyclic GMP signalling

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:46 NO synthase mechanism CSB Fig 2:46 NO synthase mechanism

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:47 eNOS activation CSB Fig 2:47 eNOS activation

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:48 Summary of redox signalling CSB Fig 2:48 Summary of redox signalling

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:49 Reactive oxygen species (ROS) CSB Fig 2:49 Reactive oxygen species (ROS)

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:50 Sites of ROS formation CSB Fig 2:50 Sites of ROS formation

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:51 Plasma membrane ROS formation CSB Fig 2:51 Plasma membrane ROS formation

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:52 GSH/GSSG couple CSB Fig 2:52 GSH/GSSG couple

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:53 H2O2 metabolism CSB Fig 2:53 H2O2 metabolism

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:54 Peroxiredoxin catalytic cycles CSB Fig 2:54 Peroxiredoxin catalytic cycles

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:55 Reversible & irreversible ROS oxidations CSB Fig 2:55 Reversible & irreversible ROS oxidations

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:56 ROS formation and action CSB Fig 2:56 ROS formation and action

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:57 Recovery of protein oxidation CSB Fig 2:57 Recovery of protein oxidation

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:58 Thimerosal-induced Ca2+ signalling CSB Fig 2:58 Thimerosal-induced Ca2+ signalling

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge Module 2:59 Figure ROS effects on Ca2+ signalling Module 2:59 Figure ROS effects on Ca2+ signalling

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:60 ROS microdomains CSB Fig 2:60 ROS microdomains

Cell Signalling Biology - Michael J. Berridge Cell Signalling Biology - Michael J. Berridge CSB Fig 2:61 ROS microdomains CSB Fig 2:61 ROS microdomains