La scienza delle misure chimiche e qualità della vita Mario Gallorini I.N.R.I.M Unità di Radiochimica e Spettroscopia c/o Università degli Studi di Pavia Torino - 25 Gennaio 2007

INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

EUROTERVIT - JRC- Ispra

INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Ce Co Cr Cs Fe Hg < Ir Ni Rb Sb Ta Zn < INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

The application of the Neutron Activation Analysis for trace metals determination in human brain tissues and substantia nigra neuromelanin In human brain many transition metals are necessary for important biological processes, and alterations in their homeostasis can produce neurotoxic effects. Toxicity pathways frequently involve oxidative stress through an increase of free radicals production (redox- reactions). The subsequent damages or death of cells can lead to neurodegenerative diseases, such as Parkinson’s Disease (PD) and Alzheimer Disease (AD) INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Trace elements analysis in human brain tissues is an important step in understanding the PD pathogenesis. Thanks to a controlled separation procedure, NM was isolated from SN with no metal contamination. NM showed a great ability to bind large amounts of metals, especially Fe, suggesting that NM can play a protective role for brain neurons against the free toxic radicals deriving from redox active metal ions. Fe and Cu concentrations in brain tissues increase along with aging in normal subjects. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia Trace metals and human health – Parkinson disease

INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Human substantia nigra in parkinsonian (A) and in normal subject (B) AB INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Substantia nigra pars compacta of normal (A) and parkinsonian subjects (B) AB INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Trace metals in human brain tissues and their neuromelanines

INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Biochemical Pharmacology, Aug 1;66(3), pp , (2003). Pigment Cell Res., 17(6):610-7., Dec; (2004). INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Fe SN LC Cu LC Cu SN LC Proc Natl Acad Sci U S A.;101(26): Epub Jun 21, (2004) INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Radiotracers in vivo experiments Nuclear reaction : p, xn Nat Ti 48 V

INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

I N R I M Toxic metals in the environment - K and P comparison of CCQM IAWG (2006) INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

I N R I M As in shellfish I N R I M INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia I N R I M

FOOD ANALYSIS : TRACE METALS DETERMINATION IN TOMATO PASTE Analytical techniques and procedures for Cadmiun determination INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

with PM10 concentration more than 75  g/m 3 … Decreto Ministeriale n° 60 del 02/02/2002

Stage 6 (0,70 – 1,08  m) Stage 7 (0,41 – 0,70  m) Stage 8 (0 – 0,41  m) Stage 5 (1,08 – 2,15  m) Stage 4 (2,15 – 3,3  m) Pre-impactor (> 9.0  m) Stage 1 (6,0 – 9,0  m) Stage 2 (4,6 – 6,0  m) Stage 3 (3,3 – 4,6  m)

Air particulate matter collected onto filters Stage 1 (  m) Stage 5 (  m) Stage 8 (  m) Total Suspended Particle (TSP)

NIST 2783 Air Particulate on Filter Media Lab. 7: IMGC Lab.12: NIST Reference Materials for life science : Trace elements in air particulate matter PM 2.5 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

NIST 2783 Air Particulate on Filter Media Lab. 7: INRIM Lab.12: NIST Reference Materials for life science : Trace elements in air particulate matter PM 2.5 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

Gaithersburg, MD Certificate Issue Date: 2 January 2002 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

YES !!