IMPROVING THE REPRODUCTIVE PERFORMANCE BY OPTIMIZING OF NUTRITION AND INTRODUCED A REPRODUCTIVE MANAGEMENT SYSTEM IN DAIRY HERDS 1 Gábor, G., 2 Koppány.

Slides:



Advertisements
Similar presentations
USE OF PREGNANCY SPECIFIC PROTEIN B TEST FOR PREGNANCY DIAGNOSIS AND ASSESMENT OF BULL FERTILITY G. Gábor, F. Tóth, N. Solymosi.
Advertisements

Synchronization. 2 wave cycle MetestrusDiestrusProestrusEstrus Day of cycle False Estrus 1.
Estrous Synchronization A management technique that makes use of hormones to control or reschedule the estrous cycle A management technique that makes.
Pregnancy and Lactation
What does the future hold for improving reproductive efficiency in dairy cattle? Matt Lucy Animal Reproductive Biology Group Division of Animal Sciences.
Hormones of pregnancy. Pregnancy Preparation of uterus –Steroid hormones Fertilization –Coitus –Gamete transfer –Capacitation of sperms –Fusion of gamates.
Female Reproductive System
Think about… 4.1 Hormonal control of the menstrual cycle 4.2 Use of hormones Recall ‘Think about…’ Summary concept map.
Puberty and associated changes
Female Reproductive System: Functions Role of male is to produce and deliver sperm. Role of female is 1. Generate and release fertile ova 2. Maintain fertilized.
Matt McMillan, Ph.D.. Includes: Vulva Vagina Cervix Uterus Oviducts Ovaries.
By C. Kohn, Waterford, WI. An animal’s body must have consistency – a body’s physiological variables must be kept within a narrow range For example, you.
Reproductive cycles. Stages and phases of the estrous cycle.
Reproductive Physiology 1)Menstrual Cycle 2)Pregnancy 3)Lactation.
ANESTRUM IN CATTLE.
Reproductive Physiology of Beef and Cattle
Colorado Agriscience Curriculum Animal Science Unit 4 – Reproduction and Genetics Lesson 4 - Female Hormones of Reproduction and Estrous.
REPEAT BREEDING SYNDROME
Beef cow reproductive management
C) The Menstrual Cycle The female menstrual cycle takes an average of 28 days. (Variation is common). The cycle occurs in four phases if no fertilization.
The Male Reproductive System
Manipulating Ovulation and Estrous Synchronization
Emily Bartlett Katrina Bush
Reproductive cycles and how they relate to breeding systems.
Manipulating Ovulation and Estrous Synchronization John Parrish.
Estrous Synchronization (Ovulation Induction) and Ovsynch
Animal Science 434 Reproductive Cycles in the Female.
The Application of Endocrinology to Selected Animals and Humans (Chapter 11) Efficient production – dependent on effective bodily function Effective bodily.
Female Reproductive Physiology. Role of the human female The female human has the following roles in reproduction –Production of the egg –Ovulation –Implantation.
CONCEPTION, DEVELOPMENT, AND BIRTH CHAPTER 46 (AP BOOK)
Heat stress round table Feb 2012 Fabio de Rensis & M.A. Driancourt.
Swine Reproduction. Breeding herds Replacement gilts Replacement gilts Sows Sows Boars Boars.
Advanced reproductive Physiology By: A. Riasi (PhD in Animal Nutrition & Physiology) فیزیولوژی تولید مثل پیشرفته.
Accelerated Biology.  Some important vocabulary  Follicle – a cluster of cells that surrounds an immature egg and provides it with nutrients (where.
Animal Science 434 Lecture 11:The Luteal Phase of the Estrous Cycle.
AA22 Animal Science II Animal Anatomy & Physiology
EARLY PREGNANCY DETECTION IN DAIRY CATTLE BY BIOPRYN ™ ELISA TEST F. Toth, G. Gabor, Fan Huang and R.G. Sasser.
Implementing Reproductive Programs Jeffrey S. Stevenson, Ph.D. Ontario Association of Bovine Practitioners Reproductive Symposium Department of Animal.
Detection of the embryonic loss in dairy cattle by ELISA tests György Gábor 1* - Fruzsina Tóth 1 – László Ózsvári 2 – Garth Sasser 3 1 Research Institute.
INFLUENCE OF SYNCHRONIZED OVULATION AND PRE- SYNCHRONIZATION ON PREGNANCY RATES USED TIMED AI IN LACTATING DAIRY COWS G. Gabor and F. Toth.
Hormonal Control of Pregnancy and Lactation. Dr. M. Alzaharna (2014) Early Embryonic Development After fertilization, the embryo spends the first four.
Jeffrey S. Stevenson, Ph.D. Department of Animal Sciences and Industry Improving Pregnancy Rates Ontario Association of Bovine Practitioners Reproductive.
Date RECEPTAL® and ESTRUMATE® - short presentation of the products features MSD AH Animal Middle East Symposium Beirut 2012 Brought to you by Partners.
AN ONLINE TUTORIAL. CONTENTS HOW TO USE THIS TUTORIAL OBJECTIVES INTRODUCTION AND DEFINITION COMPONENTS OF THE AXIS THE HYPOTHALAMUS THE PITUITARY THE.
Estrous synchronization & ovulation induction ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และ วิทยาการสืบพันธุ์
Reproductive Hormones
Updated: 11/10/2015 Estrous Synchronization (Ovulation Induction) and Ovsynch John Parrish.
Reproductive System Section 1 Reproductive and Hormonal Functions of the Male Section 2. Reproductive and Hormonal Functions of the Female Section 3. Hormonal.
Jeffrey S. Stevenson, Ph.D. Department of Animal Sciences and Industry Getting Cows Pregnant Ontario Association of Bovine Practitioners Reproductive Symposium.
The Effect of Hormones on the Estrous Cycle
Animal Science 434 Lecture 11: The Luteal Phase of the Estrous Cycle and the Menstrual Cycle in Primates.
LOGO Reproductive System YaoYang, Physiology department of SMC.
Reproductive Physiology
Estrous Synchronization (Ovulation Induction)
University of Minnesota Beef Improvement Federation
Location: Brain – at the bottom of the third ventricle Tissue Type: Neural Hormone: - Gonadotropin Releasing Hormone (GnRH) - Oxytocin Target Organ/Structure(s):
Chapter 9. THE HUMAN MENSTRUAL CYCLE Reid L. Norman, PhD Professor, Pharmacology and Neuroscience, TTUHSC.
Lecture 11: The Luteal Phase of the Estrous and Menstrual Cycle
By C. Kohn, Waterford, WI. Tissue only responds to a hormone if it has a receptor for that particular hormone works sort of like a lock and key a hormone.
INTRAUTERINE DEVICES- A HIGHLY EFFECTIVE METHOD OF CONTRACEPTION By Megan Klein.
Male and female sex hormones
Fertility and Infertility
Major endocrine systems for regulation of reproductive processes:
Hormones of the ovary 2 - Progesterone Lecture NO: 2nd MBBS
Reproductive Hormones
Ovarian and Hormonal Changes During Ovsynch Program in Buffalo-cows
Reproductive Physiology
Manipulating Ovulation and Estrous Synchronization
Increasing AI Efficiency Using Ultrasound
Animal Nutrition and Reproductive Physiology (part 1)
Presentation transcript:

IMPROVING THE REPRODUCTIVE PERFORMANCE BY OPTIMIZING OF NUTRITION AND INTRODUCED A REPRODUCTIVE MANAGEMENT SYSTEM IN DAIRY HERDS 1 Gábor, G., 2 Koppány G., 1 Tóth, F., 2 Kulik, Z., 2 Szegszárdy I. 1 Research Institute for Animal Breeding and Nutrition, H-2053 Herceghalom, Hungary, 2 Vitafort RT, H-2370 Dabas, Hungary

Why is that actual? a continuous elevation of the average milk production herds estrus detection is the main limitation for the optimal reproductive performance Optimizing reproductive performance by reproductive management is available Pharmaceutical regulation of the estrus cycle let us synchronizing estrus and ovulation, regulating follicular waves, reducing undetected heats, improving AI. Aims: Decreasing the parturition interval and the number of AI per pregnancy

Milk Producers: thousands farmers and farm Milk production: ~2 billion kg Number of milking cows ~ Average milk production per cow/year: 7600 kg Calving interval /CI/ (2004) 434 days Number of AI per pregnancy/NAIP/: 3.48 Decrease of the NAIP by 0.1 means about € savings per year. Decrease the CI by 1 day means about € savings per year. Milk production in Hungary

Biological background – easy procedure? < 90 % What’s the problem?

Causes –High milk production –Suboptimal management systems –Inadequate nutrient intake Consequences –NEBAL at parturition and low body condition –Increase the number of AI's per pregnancy –Inactive ovaries –Increase of calving interval Causes and consequences

The change of intake and require of ME and the body weight during lactation (Roberts, 1982)

Milk production and fertility of dairy cows

Effect of inadequate nutrition on the ovaries Deficit of energy intake: CL’s with cavities (physiological??) Luteal cyst Embryonic loss Deficit of protein intake: Inactive ovaries Non cycling cows High protein dietary: Increase of serum urea concentration Decrease of serum progesteron concentration Follicle cyst Embryonic loss

The aims of our management system Improving reproductive performance by optimizing nutrition. –Prevention of the negative effect of the inadequate nutrition (NEBAL, protein imbalance, vitamin, micro- elements and mineral insufficiency) –Improve BCS and decrease negative effects of NEBAL, in order to allow a normal reaction of cows for the reproductive treatments. Decrease the calving interval and the number of AI's per pregnancy.

The main elements of this management system Optimizing nutrition –Examination of the basic nutrition components (detailed chemical analysis of the feeds) –On the basis of analyses the optimized diet is calculated; energy, protein, mineral and vitamin requirements, protein and energy balance) –The diets are calculated by the milk yield. –The diets are adequate to the different dairy farms. –Continuous controlling the mixed food Optimizing the reproduction –Early pregnancy check –Controlling the reproductive cycle Synchronization of ovulation and inducing estrus –Re-breeding the open cows as soon as it possible!!

Early pregnancy detection The most common methods for the pregnancy detection –Uterine palpation per rectum (35-60 days post insemination) –Ultrasound examination (since 25 days post insemination) –Milk or serum progesterone (2-3 times days post insemination) –Different pregnancy-specific proteins (PSPB, PAG; since 25 days post insemination)

The use of transrectal ultrasonography to assess pregnancy status during early gestation is among the most practical applications of ultrasound for dairy cattle reproduction. Ultrasonography of the uterus

Early pregnancy detection by examination of the Pregnancy-specific protein B (PSPB) Pregnancy specific protein B is measured for detection of pregnancy in ruminants. BioPRYN TM, an ELISA test for PSPB, has been developed and is distributed for the detection of PSPB in the circulation of pregnant cows days after insemination. Detection of PSPB in blood provides an indication of embryonic loss as well.

Examination of the serum or milk progesterone concentration Progesterone ng/ml Days from estrus

Ultrasonography of the ovaries Application of ultrasound for monitoring the estrus cycle (examination of different ovarian structures; 40 to 60 days post partum) in order to decide the exact treatment for cows that were open after each AI.

Methods for inducing estrus and synchronizing ovulation Methods with heat detection –Progestin application (MGA, PRID, CIDR) –Deslorelin implant (GnRH agonist) –Prostaglandin (single or repeated PGf 2  im treatment) Timed insemination –Heatsynch (GnRH - PGf 2  – ECP) –Ovsynch (GnRH - PGf 2  – GnRH) –Provsynch (PGf 2  – PGf 2  – GnRH - PGf 2  – GnRH) starts 35 days after parturition

Table 1: Efficiency of the different hormonal treatments on the PR after the first AI

Table 2: Results of the new management system in practice (a HF herd with 300 cows) Milk production (kg) Calving interval (days) Number of AI per pregnancy Pregnancy rate after the first service (%)

The optimized nutrition and reproductive management system in practice Optimizing the diet for the given herd. Timed AI (Provsynch) of all cows days postpartum. Early (30-36 days post insemination) pregnancy detection by BioPryn test. Blood sera of the open cows are assayed for serum P4 level and all cows in cycle are treated immediately by a single PGF 2a injection. Non-cycling cows are put on the Ovsynch regimen and timed AI is carried out 10 days followed the first GnRH treatment.

Table 3: The cost-benefit analysis of improved fertility results in (data originated from Table 2) **Savings (€) (reduced cost) ***Cost (€) * Profit (€) Total (€) * herd level,** savings by reducing calving interval and AI costs,*** cost of products for treatment

On the basis of our experiences and practice, these regimens would help to reduce anoestrus, parturition interval, synchronize return services and enhance embryo survival. The improvement of the reproductive performance will result higher profitability, so the complex nutrition and reproductive management system is a rational method to the better economic efficiency and competitive superiority. Conclusions

Thank you for your attention!

Regulation of reproduction Interactive procedure that coordinates and regulates all reproductive functions Main elements of the regulation are: 1.Nervous system Fundamental responsibility is translating or transducing external stimuli into neural signals 2.Endocrine system Pathways are: neural reflex and neuroendocrine reflex.

Simple neural and neuroendocrine reflex (Senger, 2003)

Reproductive hormones Originate –Hypothalamus –Pituitary –Gonads –Uterus –Placenta Cause –Release of other hormones (releasing hormones) –Stimulate gonads (gonadotropins) –Sexual promotion (steroids) –Pregnancy maintenance –Luteolysis Biochemical classification –Peptides –Glycoproteins –Steroids –Prostaglandins

The most important hormones influencing ovarian activity HormoneBiochemical classification SourceAction in femaleEffect on ovary GnRH DecapeptideHypothalamusRelease FSH and LH Follicular develop- ment; ovulation LH GlycoproteinPituitaryStimulates ovulation and P4 secretion Formation of CL FSH GlycoproteinPituitaryFollicular development, E2 synthesis Development of follicles Progesterone SteroidCL, placenta Maintenance of pregnancy Inhibits GnRH release Estradiol Steroid Follicle, placenta Sexual behavior- hCG GlycoproteinChorion ovarian P4 synthesis- eCG GlycoproteinChorion Formation of accessory CL’s - PGF 2  ProstaglandinEndometriumDestruction of CLLuteolysis Inhibin Glycoprotein Granulosal cells Inhibits FSH secretionInhibits follicle development

The estrus cycle of the cow (Senger, 2003)

PSPB is a novel protein that was first reported by scientists at the University of Idaho. PSPB is located in the giant binucleate cells of the trophoblastic ectoderm of the placenta and this indicated that it was either synthesized or sequestered by those cells. Cotyledon Caruncle

Migration of these cells result the appearance of PSPB in the maternal circulation. Bi-Nucleated Cells Placenta Uterus PSPB in Circulation

PSPB ng/mL Parturition Days post breeding PSPB is detectable in serum from 24 to 282 (parturition) days of gestation and can be applied reliably in dairy cow herds at 28 to 30 days after breading.

Biopryn ELISA test – stop color reaction

Distribution of Optical Density (OD) O D cutoff