Varian 900-LC Series iHPLC

What is the iHPLC? 2 models 920-LC and 940-LC Compact, stylish, automated analytical and semi prep HPLC Pre-plumbed and factory tested for the highest confidence Easy to order, install and use Single Ethernet cable connection Focused on improved productivity through automation Updated premium performance hardware Autosampler, Fraction Collector and Scale up Module for semi-prep Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical Updated Integrated Instrument Software Interface Eliminate repetition, modernise UI Consistent Easy to use with added functionality Adds to modular range

Varian 920-LC Integrated Analytical HPLC

Varian 920-LC What Is It? Compact, stylish automated analytical HPLC Pre-plumbed and factory tested for the highest confidence Single cable communications Virtually pulse-free dual piston pump Does not require a pulse damper State-of-the-art low pressure quaternary gradient module with built-in degasser Autosampler with cooling option Synchronised injection for outstanding retention time reproducibility Compatible with 200 x 1.6 ml HPLC vials or 3 microplates

Varian 920-LC What Is It? Either dual l UV-Vis or PDA detector Optional second detector - Fluorescence or RI High precision (<0.15oC) dual column heater available Low delay solvent pre-heater for improved heat transfer Optional column switching and electric injection valve NEW improved instrument interface on GalaxieTM software External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.

Varian 920-LC Silver Bullets Premium analytical pump performance and reliability Superb Fast LC performance optimised for sub 3 um columns Improved throughput without compromising robustness High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler Full system level computer controlled AIQ (validation) Updated driver for enhanced user experience

920-LC from Conventional to Fast HPLC 25cm x 4.6mm Pursuit XRS 5um 1.0ml/min 25cm x 4.6mm Pursuit XRS 5um 2.2ml/min 15cm x 4.6mm Pursuit XRS 5um 1.0ml/min 15cm x 4.6mm Pursuit XRS 5um 3.75ml/min 10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min 5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min 16 x Speed Enhancement 5cm x 2.0mm 2.4um 1.3ml/min

Varian 920-LC Fast LC PAHs Varian 920-LC 6 minute Analysis Waters Acquity 7 Minute Analysis

Varian 920-LC Solvent Delivery System Dual piston quaternary pump produces fast gradients – even at 200 ml/min Virtually pulse-free pumping without pulse damper Outstanding gradient compositional accuracy give retention time precision at the <1% level Built-in vacuum degas maintains pump prime and enhanced injection reliability Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns Overlay of 10 chromatograms 10% steps at 1ml/min

Varian 920-LC Autosampler Needle-in-flow path geometry for low carry-over (<0.01%) Typical inj precision (< 0.3% at 10 ml) Optimized flow paths for fast injection turn-around Synchronised injection for best RT reproducibility Programmable wash setting for low carry-over Injection volumes from 1ul to 50mL Cooled option available

Varian 920-LC Washout Performance 125 ug/ml Caffeine Blank injection <0.01% carryover

Varian 920-LC Injection Synchronization

Sensitive UV-Vis Detectors Typical Agilent VWD S/N ~ 25 Typical Varian 920-LC UV/Vis S/N > 36

Information Rich Diode Array Detection 1024 pixels provides full coverage from 190 – 950 nm Full l spectra collection Enhanced purity Automatic peak identification with customised libraries Greater confidence on compound identification Choice of flowcells 4 mm path length semi-micro 9mm path length analytical Front panel access Lamps and flowcells D2 and Vis lamps self aligned Easily user maintained

Information Rich Diode Array Detection Automatic peak identification with customised libraries Use spectra as well as RT for compound ID Greater confidence on compound identification Peak search of unknown yields 998.7 match for naphthalene

Varian 920-LC Fluorescence Detector Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method) Time programmable l for increase sensitivity Automatic l validation with built-in Hg lamp Low 12 ml dead volume flowcell for improved chromatography

Varian 920-LC Refractive Index Detector Based upon 356-LC RI detector Low volume, flow efficient cell for superior peak shape Stable, long life and high intensity LED Minimises drift Provides excellent detection limits Enhanced life-time Temperature controlled optics minimises baseline drift and provides outstanding detection limits Complete automation thru Galaxie software

Universal HPLC detector Varian 380-LC and 385-LC ELSD Universal HPLC detector Compatible with gradients More sensitive than RI NEW Glass Nebuliser for Improved Reproducibility 2 to 3 Times More Sensitive 40Hz Data Rate for Fast LC Complete Galaxie compatibility Program changes thru a run

Heated Column Compartment Temperature range from ambient + 10oC up to 65oC Excellent temperature precision +0.15oC Ensures retention time reproducibility Eliminates re-runs Good temperature accuracy <1oC Low delay volume pre-heater for efficient heat transfer to mobile phase Rapid warm-up Typically from ambient to 40oc in <15 minutes Spacious - easily accommodates 2 x 250mm columns and 2 valves Option with electrically actuated column switching valve and injection valve

Excellent Long term Stability

Quick Warmup - Outstanding Stability

Reproducibility of 50 Injections Reproducibility <0.8% over 50 injections and 12 hours

Varian 920-LC Software Usability User friendly GalaxieTM software Simplified, modern, consistent Instrument interface Modular 11 pages 920-LC 4 pages!! One page real-time status and chromatogram Advanced integration and calibration New IMS capabilities Automated instrument validation Reduces downtime and operating costs Fusion AE

Varian 920-LC Integrated HPLC Special features of the Varian 920-LC Leak detector Increases confidence with unattended automated runs Single waste point Ensures capture of all wastes Easy to connect to waste vessel Built-in Solvent bottle storage Improved safety and convenience Lower hight than modular systems

Varian 920-LC Integrated HPLC Special features of the Varian 920-LC Clear front doors Stylish Provides access to autosampler without opening Safety increased as lab staff shielded from solvent spray and leaks Useful, convenient storage compartments Column rack in door Tool drawer Operation log drawer

Varian 920-LC AIQ (Validation) AIQ (Analytical Instrument Qualification) Fully PC controlled IQ and OQ (not PQ) Puts the Varian 920-LC at the forefront of regulated capabilities Consistent, reproducible methodology Includes pumps, autosampler, detectors, column heater Chargeable option Fully documented

900 LC -Series AIQ Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package. Verifies the entire LC system by systematically running the following Flow Rate Accuracy Test Wavelength Detector Accuracy Test Auto-sampler Injector Accuracy Test (also measures Detector Linearity) Auto-sampler Injector Precision Test Auto-sampler Injector Carryover Test Proportioning Accuracy Test Detector Noise Test Detector Drift Test Auto-sampler Vial Identification Accuracy Test Proportioning Linearity Compositional Ripple

Varian 920-LC Configurations pump inject detector Oven Detector 2 option Options Isocratic 00-100890-00 Dual piston pump 10 ml head Auto Dual l UV/Vis Opt FL, RI ELSD Gradient 00-100891-00 Quat, Grad, Degas ELSD opt High Performance Gradient 00-100892-00 Auto cooled Development 00-100893-00 PDA Std Research 00-100894-00

Varian 920-LC Consumables Excellent range of HPLC Columns providing industry leading resolution and robustness Pursuit Pursuit XRS Polymeric Phases - PL From 2mm to 2” ID Prepacked Other Speciality Phases NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC 2.8um Ultra is optimised for Speed 2.4um gives further enhancements in Resolution

2.8um Vs 2.4 um – High Res or Extra Speed 2.4um 3mm x 5cm for RESOLUTION 2.8um 2mm x 5cm for speed

Questions??