A. Malcolm Campbell Biology and GCAT Laurie J. Heyer Mathematics and GCAT AP Institute June 29, 2011 Introduction to Synthetic Biology Todd T. Eckdahl.

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Presentation transcript:

A. Malcolm Campbell Biology and GCAT Laurie J. Heyer Mathematics and GCAT AP Institute June 29, 2011 Introduction to Synthetic Biology Todd T. Eckdahl Biology and GCAT Jeff L. Poet Mathematics and GCAT

What is Synthetic Biology? Application of engineering principles and mathematical modeling to the design and construction of biological parts, devices, and systems with applications in energy, medicine, and technology.

Synthetic Biology: Win-Win Win #1: your design functions as expected.

Win #2: your design fails but you uncover basic biology Win #1: your design functions as expected. Synthetic Biology: Win-Win Research

Abstraction Modularity Standards Designing and modeling How is Synthetic Biology Different?

Abstraction

USB ports on computers Modularity

Standardization On a Uniform System of Screw Thread William Sellers April 21, 1864 “In this country, no organized attempt has as of yet been made to establish any system, each manufacturer having adopted whatever his judgment may have dictated as best, or as most convenient for himself.”

On a Uniform System of Screw Thread Standardization

Modeling of Designs

Real World Applications of Synthetic Biology

Land Mine Detection

Synthetic Biology Land Mine Detection

Production of Medicines $1 per pill

Production of Medicines 10¢ per pill

Biofuels from Algae CO -neutral 2 1,000,000 gallons in 2008

Synthetic Biology Biochemistry Synthetic Biology Computer Science Engineering

Synthetic Biology

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UC Berkeley iGEM, 2007 Synthetic Biology

BioBricks BioBrick Part BioBrick plasmid backbone BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS (

Eco RI BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS ( Spe I Pst I Xba I BioBricks

Eco RI BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS ( Spe I Pst I Xba I identical sticky ends BioBricks

PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S put B downstream of A BioBricks

PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S cut with Spe and Pst cut with Xba and Pst BioBricks

P S Part A originantibiotic resistance X E Part B X P S gel purify EtOH precipitate BioBricks

P S Part A originantibiotic resistance X E Part B X P S ligate BioBricks

S Part A originantibiotic resistance X E Part B X S P M mixed site = scar transform BioBricks

S Part A originantibiotic resistance X E Part B X S P M PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S BioBricks

PS Part A originantibiotic resistance originantibiotic resistance E X E Assignment: Part B X P S put A upstream of B